Circulating red cell-derived microparticles in human malaria

Nantakomol, D; Dondorp, AM; Krudsood, S; Udomsangpetch, R; Pattanapanyasat, K; Combes, V; Grau, GE; White, NJ; Viriyavejakul, P; Day, NPJ; Chotivanich, K

Circulating red cell-derived microparticles in human malaria

Nantakomol, D Dondorp, AM Krudsood, S Udomsangpetch, R Pattanapanyasat, K Combes, V

Grau, GE White, NJ Viriyavejakul, P Day, NPJ Chotivanich, K

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Publication Type:: Journal Article
Citation:: Journal of Infectious Diseases, 2011, 203 (5), pp. 700 - 706
Issue Date:: 2011-03-01

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Field	Value	Language
dc.contributor.author	Nantakomol, D	en_US
dc.contributor.author	Dondorp, AM	en_US
dc.contributor.author	Krudsood, S	en_US
dc.contributor.author	Udomsangpetch, R	en_US
dc.contributor.author	Pattanapanyasat, K	en_US
dc.contributor.author	Combes, V https://orcid.org/0000-0003-2178-3596	en_US
dc.contributor.author	Grau, GE	en_US
dc.contributor.author	White, NJ	en_US
dc.contributor.author	Viriyavejakul, P	en_US
dc.contributor.author	Day, NPJ	en_US
dc.contributor.author	Chotivanich, K	en_US
dc.date.issued	2011-03-01	en_US
dc.identifier.citation	Journal of Infectious Diseases, 2011, 203 (5), pp. 700 - 706	en_US
dc.identifier.issn	0022-1899	en_US
dc.identifier.uri	http://hdl.handle.net/10453/109929
dc.description.abstract	In patients with falciparum malaria, plasma concentrations of cell-derived microparticles correlate with disease severity. Using flow cytometry, we quantified red blood cell-derived microparticles (RMPs) in patients with malaria and identified the source and the factors associated with production. RMP concentrations were increased in patients with Plasmodium falciparum (n = 29; median, 457 RMPs/μL [range, 13-4,342 RMPs/μL]), Plasmodium vivax (n = 5; median, 409 RMPs/μL [range, 281-503/μL]), and Plasmodium malariae (n = 2; median, 163 RMPs/μL [range, 127-200 RMPs/μL]) compared with those in healthy subjects (n = 11; median, 8 RMPs/μL [range, 3-166 RMPs/μL]; P = .01). RMP concentrations were highest in patients with severe falciparum malaria (P=.01). Parasitized red cells produced >10 times more RMPs than did unparasitized cells, but the overall majority of RMPs still derived from uninfected red blood cells (URBCs). In cultures, RMP production increased as the parasites matured. Hemin and parasite products induced RMP production in URBCs, which was inhibited by N-acetylcysteine, suggesting heme-mediated oxidative stress as a pathway for the generation of RMPs. © The Author 2011.	en_US
dc.relation.ispartof	Journal of Infectious Diseases	en_US
dc.relation.isbasedon	10.1093/infdis/jiq104	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Plasmodium	en_US
dc.subject.mesh	Malaria	en_US
dc.subject.mesh	Antimalarials	en_US
dc.subject.mesh	Case-Control Studies	en_US
dc.subject.mesh	Thailand	en_US
dc.subject.mesh	Cell-Derived Microparticles	en_US
dc.title	Circulating red cell-derived microparticles in human malaria	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	203	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	203	en_US

Abstract:

In patients with falciparum malaria, plasma concentrations of cell-derived microparticles correlate with disease severity. Using flow cytometry, we quantified red blood cell-derived microparticles (RMPs) in patients with malaria and identified the source and the factors associated with production. RMP concentrations were increased in patients with Plasmodium falciparum (n = 29; median, 457 RMPs/μL [range, 13-4,342 RMPs/μL]), Plasmodium vivax (n = 5; median, 409 RMPs/μL [range, 281-503/μL]), and Plasmodium malariae (n = 2; median, 163 RMPs/μL [range, 127-200 RMPs/μL]) compared with those in healthy subjects (n = 11; median, 8 RMPs/μL [range, 3-166 RMPs/μL]; P = .01). RMP concentrations were highest in patients with severe falciparum malaria (P=.01). Parasitized red cells produced >10 times more RMPs than did unparasitized cells, but the overall majority of RMPs still derived from uninfected red blood cells (URBCs). In cultures, RMP production increased as the parasites matured. Hemin and parasite products induced RMP production in URBCs, which was inhibited by N-acetylcysteine, suggesting heme-mediated oxidative stress as a pathway for the generation of RMPs. © The Author 2011.

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http://hdl.handle.net/10453/109929