Desiccation induced oxidative stress and its biochemical responses in intertidal red alga Gracilaria corticata (Gracilariales, Rhodophyta)

Kumar, M; Gupta, V; Trivedi, N; Kumari, P; Bijo, AJ; Reddy, CRK; Jha, B

Desiccation induced oxidative stress and its biochemical responses in intertidal red alga Gracilaria corticata (Gracilariales, Rhodophyta)

Kumar, M

Gupta, V Trivedi, N Kumari, P Bijo, AJ Reddy, CRK Jha, B

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Publication Type:: Journal Article
Citation:: Environmental and Experimental Botany, 2011, 72 (2), pp. 194 - 201
Issue Date:: 2011-09-01

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Field	Value	Language
dc.contributor.author	Kumar, M https://orcid.org/0000-0002-5247-3059	en_US
dc.contributor.author	Gupta, V	en_US
dc.contributor.author	Trivedi, N	en_US
dc.contributor.author	Kumari, P	en_US
dc.contributor.author	Bijo, AJ	en_US
dc.contributor.author	Reddy, CRK	en_US
dc.contributor.author	Jha, B	en_US
dc.date.issued	2011-09-01	en_US
dc.identifier.citation	Environmental and Experimental Botany, 2011, 72 (2), pp. 194 - 201	en_US
dc.identifier.issn	0098-8472	en_US
dc.identifier.uri	http://hdl.handle.net/10453/109956
dc.description.abstract	Intertidal alga Gracilaria corticata growing in natural environment experiences various abiotic stresses during the low tides. The aim of this study was to determine whether desiccation exposure would lead to oxidative stress and its effect varies with exposure periods. This study gives an account of various biochemical changes in G. corticata following the exposure to desiccation for a period of 0 (control), 1, 2, 3 and 4. h under controlled conditions. During desiccation, G. corticata thalli showed dramatic loss of water by almost 47% when desiccated for 4. h. The enhanced production of reactive oxygen species (ROS) and increased lipid peroxidation observed during the exposure of 3-4. h were chiefly contributed by higher lipoxygenase (LOX) activity with the induction of two new LOX isoforms (LOX-2, ~85. kDa; LOX-3, ~65. kDa). The chlorophyll, carotenoids and phycobiliproteins (phycoerythrin and phycocyanin) were increased during initial 2. h exposure compared to control and thereafter declined in the succeeding exposure. The antioxidative enzymes such as superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), glutathione peroxidase (GPX) and the regeneration rate of reduced ascorbate (AsA) and glutathione (GSH) increased during desiccation up to 2-3. h. Further, the isoforms of antioxidant enzymes Mn-SOD (~150. kDa), APX-4 (~110. kDa), APX-5 (~45. kDa), GPX-1 (~80. kDa) and GPX-2 (~65. kDa) responded specifically to the desiccation exposure. Compared to control, a relative higher content of both free and bound insoluble putrescine and spermine together with enhanced n-6 PUFAs namely C20:4(n-6) and C20:3(n-6) fatty acids found during 2. h exposure reveals their involvement in defence reactions against the desiccation induced oxidative stress. © 2011 Elsevier B.V.	en_US
dc.relation.ispartof	Environmental and Experimental Botany	en_US
dc.relation.isbasedon	10.1016/j.envexpbot.2011.03.007	en_US
dc.subject.classification	Plant Biology & Botany	en_US
dc.title	Desiccation induced oxidative stress and its biochemical responses in intertidal red alga Gracilaria corticata (Gracilariales, Rhodophyta)	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	72	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	72	en_US

Abstract:

Intertidal alga Gracilaria corticata growing in natural environment experiences various abiotic stresses during the low tides. The aim of this study was to determine whether desiccation exposure would lead to oxidative stress and its effect varies with exposure periods. This study gives an account of various biochemical changes in G. corticata following the exposure to desiccation for a period of 0 (control), 1, 2, 3 and 4. h under controlled conditions. During desiccation, G. corticata thalli showed dramatic loss of water by almost 47% when desiccated for 4. h. The enhanced production of reactive oxygen species (ROS) and increased lipid peroxidation observed during the exposure of 3-4. h were chiefly contributed by higher lipoxygenase (LOX) activity with the induction of two new LOX isoforms (LOX-2, ~85. kDa; LOX-3, ~65. kDa). The chlorophyll, carotenoids and phycobiliproteins (phycoerythrin and phycocyanin) were increased during initial 2. h exposure compared to control and thereafter declined in the succeeding exposure. The antioxidative enzymes such as superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), glutathione peroxidase (GPX) and the regeneration rate of reduced ascorbate (AsA) and glutathione (GSH) increased during desiccation up to 2-3. h. Further, the isoforms of antioxidant enzymes Mn-SOD (~150. kDa), APX-4 (~110. kDa), APX-5 (~45. kDa), GPX-1 (~80. kDa) and GPX-2 (~65. kDa) responded specifically to the desiccation exposure. Compared to control, a relative higher content of both free and bound insoluble putrescine and spermine together with enhanced n-6 PUFAs namely C20:4(n-6) and C20:3(n-6) fatty acids found during 2. h exposure reveals their involvement in defence reactions against the desiccation induced oxidative stress. © 2011 Elsevier B.V.

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