Simultaneous determination of different endogenetic plant growth regulators in common green seaweeds using dispersive liquid-liquid microextraction method

Gupta, V; Kumar, M; Brahmbhatt, H; Reddy, CRK; Seth, A; Jha, B

Simultaneous determination of different endogenetic plant growth regulators in common green seaweeds using dispersive liquid-liquid microextraction method

Gupta, V Kumar, M

Brahmbhatt, H Reddy, CRK Seth, A Jha, B

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Publication Type:: Journal Article
Citation:: Plant Physiology and Biochemistry, 2011, 49 (11), pp. 1259 - 1263
Issue Date:: 2011-11-01

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Field	Value	Language
dc.contributor.author	Gupta, V	en_US
dc.contributor.author	Kumar, M https://orcid.org/0000-0002-5247-3059	en_US
dc.contributor.author	Brahmbhatt, H	en_US
dc.contributor.author	Reddy, CRK	en_US
dc.contributor.author	Seth, A	en_US
dc.contributor.author	Jha, B	en_US
dc.date.available	2011-08-11	en_US
dc.date.issued	2011-11-01	en_US
dc.identifier.citation	Plant Physiology and Biochemistry, 2011, 49 (11), pp. 1259 - 1263	en_US
dc.identifier.issn	0981-9428	en_US
dc.identifier.uri	http://hdl.handle.net/10453/110161
dc.description.abstract	A simple and rapid HPLC-based method was developed for simultaneous determination of major classes of plant growth regulators (PGRs) in Monostroma and different species of Ulva. The plant growth regulators determined included gibberellic acid (GA3), indole-3-acetic acid (IAA), abscisic acid (ABA), indole-3-butyric acid (IBA), salicylic acid and kinetin riboside (KR) and their respective elution time was 2.75, 3.3, 3.91, 4.95, 5.39 and 6.59min. The parameters optimized for distinct separation of PGRs were mobile phase (60:40 methanol and 0.6% acetic acid in water), column temperature (35°C) and flow rate (1ml/min). This method presented an excellent linearity (0.2-100μg/ml) with limit of detection (LOD) as 0.2μg/ml for ABA, 0.5μg/ml for KR and salicylic acid, and 1μg/ml for IAA, IBA and GA3. The precision and accuracy of the method was evaluated after inter and intra day analysis in triplicates. The effect of plant matrix was compensated after spiking and the resultant recoveries estimated were in the range of 80-120%. Each PGR thereby detected were further characterized by ESI-MS analysis. The method optimized in this study determined IBA along with IAA for the first time in the seaweed species investigated except Ulva linza where the former was not detected. In all the species studied, ABA level was detected to be the highest while kinetin riboside was the lowest. In comparison to earlier methods of PGR analysis, sample preparation and analysis time were substantially reduced while allowing determination of more classes of PGRs simultaneously. © 2011 Elsevier Masson SAS.	en_US
dc.relation.ispartof	Plant Physiology and Biochemistry	en_US
dc.relation.isbasedon	10.1016/j.plaphy.2011.08.004	en_US
dc.subject.classification	Plant Biology & Botany	en_US
dc.subject.mesh	Ulva	en_US
dc.subject.mesh	Salicylic Acid	en_US
dc.subject.mesh	Abscisic Acid	en_US
dc.subject.mesh	Gibberellins	en_US
dc.subject.mesh	Indoleacetic Acids	en_US
dc.subject.mesh	Indoles	en_US
dc.subject.mesh	Plant Growth Regulators	en_US
dc.subject.mesh	Adenosine	en_US
dc.subject.mesh	Plant Extracts	en_US
dc.subject.mesh	Chromatography, High Pressure Liquid	en_US
dc.subject.mesh	Spectrometry, Mass, Electrospray Ionization	en_US
dc.subject.mesh	Linear Models	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Reproducibility of Results	en_US
dc.subject.mesh	Time Factors	en_US
dc.subject.mesh	India	en_US
dc.subject.mesh	Kinetin	en_US
dc.subject.mesh	Chlorophyta	en_US
dc.subject.mesh	Liquid Phase Microextraction	en_US
dc.title	Simultaneous determination of different endogenetic plant growth regulators in common green seaweeds using dispersive liquid-liquid microextraction method	en_US
dc.type	Journal Article
utslib.citation.volume	11	en_US
utslib.citation.volume	49	en_US
utslib.for	0503 Soil Sciences	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0301 Analytical Chemistry	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	11	en_US
pubs.publication-status	Published	en_US
pubs.volume	49	en_US

Abstract:

A simple and rapid HPLC-based method was developed for simultaneous determination of major classes of plant growth regulators (PGRs) in Monostroma and different species of Ulva. The plant growth regulators determined included gibberellic acid (GA3), indole-3-acetic acid (IAA), abscisic acid (ABA), indole-3-butyric acid (IBA), salicylic acid and kinetin riboside (KR) and their respective elution time was 2.75, 3.3, 3.91, 4.95, 5.39 and 6.59min. The parameters optimized for distinct separation of PGRs were mobile phase (60:40 methanol and 0.6% acetic acid in water), column temperature (35°C) and flow rate (1ml/min). This method presented an excellent linearity (0.2-100μg/ml) with limit of detection (LOD) as 0.2μg/ml for ABA, 0.5μg/ml for KR and salicylic acid, and 1μg/ml for IAA, IBA and GA3. The precision and accuracy of the method was evaluated after inter and intra day analysis in triplicates. The effect of plant matrix was compensated after spiking and the resultant recoveries estimated were in the range of 80-120%. Each PGR thereby detected were further characterized by ESI-MS analysis. The method optimized in this study determined IBA along with IAA for the first time in the seaweed species investigated except Ulva linza where the former was not detected. In all the species studied, ABA level was detected to be the highest while kinetin riboside was the lowest. In comparison to earlier methods of PGR analysis, sample preparation and analysis time were substantially reduced while allowing determination of more classes of PGRs simultaneously. © 2011 Elsevier Masson SAS.

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http://hdl.handle.net/10453/110161