An alkali-halotolerant cellulase from Bacillus flexus isolated from green seaweed Ulva lactuca

Trivedi, N; Gupta, V; Kumar, M; Kumari, P; Reddy, CRK; Jha, B

An alkali-halotolerant cellulase from Bacillus flexus isolated from green seaweed Ulva lactuca

Trivedi, N Gupta, V Kumar, M

Kumari, P Reddy, CRK Jha, B

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Publication Type:: Journal Article
Citation:: Carbohydrate Polymers, 2011, 83 (2), pp. 891 - 897
Issue Date:: 2011-01-10

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Field	Value	Language
dc.contributor.author	Trivedi, N	en_US
dc.contributor.author	Gupta, V	en_US
dc.contributor.author	Kumar, M https://orcid.org/0000-0002-5247-3059	en_US
dc.contributor.author	Kumari, P	en_US
dc.contributor.author	Reddy, CRK	en_US
dc.contributor.author	Jha, B	en_US
dc.date.issued	2011-01-10	en_US
dc.identifier.citation	Carbohydrate Polymers, 2011, 83 (2), pp. 891 - 897	en_US
dc.identifier.issn	0144-8617	en_US
dc.identifier.uri	http://hdl.handle.net/10453/110163
dc.description.abstract	An extracellular alkali-halotolerant cellulase from the strain Bacillus flexus NT isolated from Ulva lactuca was purified to homogeneity with a recovery of 25.03% and purity fold of 22.31. The molecular weight of the enzyme was about 97 kDa and the Vmax and Km was 370.17 U/ml/min and 6.18 mg/ml respectively. The optimum pH and temperature for enzyme activity was 10 and 45 °C respectively. The enzymatic hydrolysis of the CMC was confirmed with GPC and GC-MS analysis. The stabilized activity of the enzyme even at high pH of 9.0-12.0 and residual activity of about 70% at salt concentration (NaCl 15%) revealed for its alkali-halotolerance nature. The metal ions Cd 2+ and Li1+ were found as inducers while Cr2+, Co2+, Zn2+ and metal chelator EDTA have significantly inhibited the enzyme activity. Enzyme activity was insensitive to ethanol and isopropanol while partially inhibited by acetone, cyclohexane and benzene. © 2010 Elsevier Ltd. All rights reserved.	en_US
dc.relation.ispartof	Carbohydrate Polymers	en_US
dc.relation.isbasedon	10.1016/j.carbpol.2010.08.069	en_US
dc.subject.classification	Polymers	en_US
dc.title	An alkali-halotolerant cellulase from Bacillus flexus isolated from green seaweed Ulva lactuca	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	83	en_US
utslib.for	0303 Macromolecular and Materials Chemistry	en_US
utslib.for	0305 Organic Chemistry	en_US
utslib.for	0908 Food Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	83	en_US

Abstract:

An extracellular alkali-halotolerant cellulase from the strain Bacillus flexus NT isolated from Ulva lactuca was purified to homogeneity with a recovery of 25.03% and purity fold of 22.31. The molecular weight of the enzyme was about 97 kDa and the Vmax and Km was 370.17 U/ml/min and 6.18 mg/ml respectively. The optimum pH and temperature for enzyme activity was 10 and 45 °C respectively. The enzymatic hydrolysis of the CMC was confirmed with GPC and GC-MS analysis. The stabilized activity of the enzyme even at high pH of 9.0-12.0 and residual activity of about 70% at salt concentration (NaCl 15%) revealed for its alkali-halotolerance nature. The metal ions Cd 2+ and Li1+ were found as inducers while Cr2+, Co2+, Zn2+ and metal chelator EDTA have significantly inhibited the enzyme activity. Enzyme activity was insensitive to ethanol and isopropanol while partially inhibited by acetone, cyclohexane and benzene. © 2010 Elsevier Ltd. All rights reserved.

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http://hdl.handle.net/10453/110163