An alkali-halotolerant cellulose from Bacillus flexus isolated from green seaweed Ulva lactuca.

Publisher:
Elsevier
Publication Type:
Journal Article
Citation:
Carbohydrate Polymers, 2011, 83 (2), pp. 891 - 897
Issue Date:
2011-01-10
Full metadata record
Files in This Item:
Filename Description Size
Bf.pdfPublished Version786.54 kB
Adobe PDF
Abstract An extracellular alkali-halotolerant cellulase from the strain Bacillus flexus NT isolated from Ulva lactuca was purified to homogeneity with a recovery of 25.03% and purity fold of 22.31. The molecular weight of the enzyme was about 97 kDa and the Vmax and Km was 370.17 U/ml/min and 6.18 mg/ml respectively. The optimum pH and temperature for enzyme activity was 10 and 45 °C respectively. The enzymatic hydrolysis of the CMC was confirmed with GPC and GC-MS analysis. The stabilized activity of the enzyme even at high pH of 9.0–12.0 and residual activity of about 70% at salt concentration (NaCl 15%) revealed for its alkali-halotolerance nature. The metal ions Cd2+ and Li1+ were found as inducers while Cr2+, Co2+, Zn2+ and metal chelator EDTA have significantly inhibited the enzyme activity. Enzyme activity was insensitive to ethanol and isopropanol while partially inhibited by acetone, cyclohexane and benzene.
Please use this identifier to cite or link to this item: