Growth and agarose characteristics of isomorphic gametophyte (male and female) and sporophyte of Gracilaria dura and their marker assisted selection

Gupta, V; Baghel, RS; Kumar, M; Kumari, P; Mantri, VA; Reddy, CRK; Jha, B

Growth and agarose characteristics of isomorphic gametophyte (male and female) and sporophyte of Gracilaria dura and their marker assisted selection

Gupta, V Baghel, RS Kumar, M

Kumari, P Mantri, VA Reddy, CRK Jha, B

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Publication Type:: Journal Article
Citation:: Aquaculture, 2011, 318 (3-4), pp. 389 - 396
Issue Date:: 2011-08-08

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Field	Value	Language
dc.contributor.author	Gupta, V	en_US
dc.contributor.author	Baghel, RS	en_US
dc.contributor.author	Kumar, M https://orcid.org/0000-0002-5247-3059	en_US
dc.contributor.author	Kumari, P	en_US
dc.contributor.author	Mantri, VA	en_US
dc.contributor.author	Reddy, CRK	en_US
dc.contributor.author	Jha, B	en_US
dc.date.issued	2011-08-08	en_US
dc.identifier.citation	Aquaculture, 2011, 318 (3-4), pp. 389 - 396	en_US
dc.identifier.issn	0044-8486	en_US
dc.identifier.uri	http://hdl.handle.net/10453/110167
dc.description.abstract	The characteristics of agarose and growth for three isomorphic life phases of G. dura with their bio-molecular marker assisted selection have been described in this study. The tetrasporophyte showed superior quality of agarose over gametophytes and recorded growth rate was highest for females. The genetic relatedness studied with ISSR markers showed quadratic line of correlation between these phases (R2=1). Their genetic diversity determinants as percentage of polymorphic loci (PPL), average heterozygosity (He) and Shannon's Weaver index (I) were 55.55%, 0.5±0.07 and 0.33 respectively. The cytological analysis for chromosome count revealed 8 chromosomes in haploid gametophytic thallus (N) and 16 for diploid tetrasporophyte (2N) together with genetic structure analysis confirmed to their sexual mating behaviour. Their marker assisted selection based on ISSR generated characteristic band of 430bp specific to male, 860bp for female and two bands of 800 and 1600bp for tetrasporophytic thallus from primer 'A'. Similarly ISSR primer 'E' also generated bands specific to male, female and tetrasporophytes while others gave bands specific to either of life phase. Interestingly, endogenic ABA content was significantly higher for haploid gametophytes (female more than male) than diploid tetrasporophyte while no significant difference was observed in IBA content. Thus the study described not only the features of three life phases of G. dura but also reliable biomarkers for differentiating such isomorphic life phases which could be beneficial for the selection of cultivar and in breeding programmes. © 2011 Elsevier B.V.	en_US
dc.relation.ispartof	Aquaculture	en_US
dc.relation.isbasedon	10.1016/j.aquaculture.2011.06.009	en_US
dc.subject.classification	Fisheries	en_US
dc.title	Growth and agarose characteristics of isomorphic gametophyte (male and female) and sporophyte of Gracilaria dura and their marker assisted selection	en_US
dc.type	Journal Article
utslib.citation.volume	3-4	en_US
utslib.citation.volume	318	en_US
utslib.for	0704 Fisheries Sciences	en_US
utslib.for	0608 Zoology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	3-4	en_US
pubs.publication-status	Published	en_US
pubs.volume	318	en_US

Abstract:

The characteristics of agarose and growth for three isomorphic life phases of G. dura with their bio-molecular marker assisted selection have been described in this study. The tetrasporophyte showed superior quality of agarose over gametophytes and recorded growth rate was highest for females. The genetic relatedness studied with ISSR markers showed quadratic line of correlation between these phases (R2=1). Their genetic diversity determinants as percentage of polymorphic loci (PPL), average heterozygosity (He) and Shannon's Weaver index (I) were 55.55%, 0.5±0.07 and 0.33 respectively. The cytological analysis for chromosome count revealed 8 chromosomes in haploid gametophytic thallus (N) and 16 for diploid tetrasporophyte (2N) together with genetic structure analysis confirmed to their sexual mating behaviour. Their marker assisted selection based on ISSR generated characteristic band of 430bp specific to male, 860bp for female and two bands of 800 and 1600bp for tetrasporophytic thallus from primer 'A'. Similarly ISSR primer 'E' also generated bands specific to male, female and tetrasporophytes while others gave bands specific to either of life phase. Interestingly, endogenic ABA content was significantly higher for haploid gametophytes (female more than male) than diploid tetrasporophyte while no significant difference was observed in IBA content. Thus the study described not only the features of three life phases of G. dura but also reliable biomarkers for differentiating such isomorphic life phases which could be beneficial for the selection of cultivar and in breeding programmes. © 2011 Elsevier B.V.

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http://hdl.handle.net/10453/110167