Solvent tolerant marine bacterium Bacillus aquimaris secreting organic solvent stable alkaline cellulase

Trivedi, N; Gupta, V; Kumar, M; Kumari, P; Reddy, CRK; Jha, B

Solvent tolerant marine bacterium Bacillus aquimaris secreting organic solvent stable alkaline cellulase

Trivedi, N Gupta, V Kumar, M

Kumari, P Reddy, CRK Jha, B

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Publication Type:: Journal Article
Citation:: Chemosphere, 2011, 83 (5), pp. 706 - 712
Issue Date:: 2011-04-01

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Field	Value	Language
dc.contributor.author	Trivedi, N	en_US
dc.contributor.author	Gupta, V	en_US
dc.contributor.author	Kumar, M https://orcid.org/0000-0002-5247-3059	en_US
dc.contributor.author	Kumari, P	en_US
dc.contributor.author	Reddy, CRK	en_US
dc.contributor.author	Jha, B	en_US
dc.date.available	2011-02-01	en_US
dc.date.issued	2011-04-01	en_US
dc.identifier.citation	Chemosphere, 2011, 83 (5), pp. 706 - 712	en_US
dc.identifier.issn	0045-6535	en_US
dc.identifier.uri	http://hdl.handle.net/10453/110334
dc.description.abstract	The organic solvent tolerant bacteria with their physiological abilities to decontaminate the organic pollutants have potentials to secrete extracellular enzymes of commercial importance. Of the 19 marine bacterial isolates examined for their solvent tolerance at 10vol.% concentration, one had the significant tolerance and showed a relative growth yield of 86% for acetone, 71% for methanol, 52% for benzene, 35% for heptane, 24% for toluene and 19% for ethylacetate. The phylogenetic analysis of this strain using 16S rDNA sequence revealed 99% homology with Bacillus aquimaris. The cellulase enzyme secreted by this strain under normal conditions showed an optimum activity at pH 11 and 45°C. The enzyme did show functional stability even at higher pH (12) and temperature (75°C) with residual activity of 85% and 95% respectively. The enzyme activity in the presence of different additives were in the following order: Co+2>Fe+2>NaOCl2>CuSO4>KCl>NaCl. The enzyme stability in the presence of solvents at 20vol.% concentration was highest in benzene with 122% followed by methanol (85%), acetone (75%), toluene (73%) and heptane (42%). The pre-incubation of enzyme in ionic liquids such as 1-ethyl-3-methylimidazolium methanesulfonate and 1-ethyl-3-methylimidazolium bromide increased its activity to 150% and 155% respectively. The change in fatty acid profile with different solvents further elucidated the physiological adaptations of the strain to tolerate such extreme conditions. © 2011 Elsevier Ltd.	en_US
dc.relation.ispartof	Chemosphere	en_US
dc.relation.isbasedon	10.1016/j.chemosphere.2011.02.006	en_US
dc.subject.classification	Environmental Sciences	en_US
dc.subject.classification	Meteorology & Atmospheric Sciences	en_US
dc.subject.mesh	Bacillus	en_US
dc.subject.mesh	Cellulase	en_US
dc.subject.mesh	Fatty Acids	en_US
dc.subject.mesh	Solvents	en_US
dc.subject.mesh	Water Pollutants, Chemical	en_US
dc.subject.mesh	Seawater	en_US
dc.subject.mesh	Adaptation, Physiological	en_US
dc.subject.mesh	Hydrogen-Ion Concentration	en_US
dc.subject.mesh	Ionic Liquids	en_US
dc.title	Solvent tolerant marine bacterium Bacillus aquimaris secreting organic solvent stable alkaline cellulase	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	83	en_US
utslib.for	039901 Environmental Chemistry (incl. Atmospheric Chemistry)	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	83	en_US

Abstract:

The organic solvent tolerant bacteria with their physiological abilities to decontaminate the organic pollutants have potentials to secrete extracellular enzymes of commercial importance. Of the 19 marine bacterial isolates examined for their solvent tolerance at 10vol.% concentration, one had the significant tolerance and showed a relative growth yield of 86% for acetone, 71% for methanol, 52% for benzene, 35% for heptane, 24% for toluene and 19% for ethylacetate. The phylogenetic analysis of this strain using 16S rDNA sequence revealed 99% homology with Bacillus aquimaris. The cellulase enzyme secreted by this strain under normal conditions showed an optimum activity at pH 11 and 45°C. The enzyme did show functional stability even at higher pH (12) and temperature (75°C) with residual activity of 85% and 95% respectively. The enzyme activity in the presence of different additives were in the following order: Co+2>Fe+2>NaOCl2>CuSO4>KCl>NaCl. The enzyme stability in the presence of solvents at 20vol.% concentration was highest in benzene with 122% followed by methanol (85%), acetone (75%), toluene (73%) and heptane (42%). The pre-incubation of enzyme in ionic liquids such as 1-ethyl-3-methylimidazolium methanesulfonate and 1-ethyl-3-methylimidazolium bromide increased its activity to 150% and 155% respectively. The change in fatty acid profile with different solvents further elucidated the physiological adaptations of the strain to tolerate such extreme conditions. © 2011 Elsevier Ltd.

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