Intramacrophage survival of uropathogenic Escherichia coli: Differences between diverse clinical isolates and between mouse and human macrophages

Bokil, NJ; Totsika, M; Carey, AJ; Stacey, KJ; Hancock, V; Saunders, BM; Ravasi, T; Ulett, GC; Schembri, MA; Sweet, MJ

Intramacrophage survival of uropathogenic Escherichia coli: Differences between diverse clinical isolates and between mouse and human macrophages

Bokil, NJ

Totsika, M Carey, AJ Stacey, KJ Hancock, V Saunders, BM

Ravasi, T Ulett, GC Schembri, MA Sweet, MJ

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Publication Type:: Journal Article
Citation:: Immunobiology, 2011, 216 (11), pp. 1164 - 1171
Issue Date:: 2011-11-01

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Field	Value	Language
dc.contributor.author	Bokil, NJ https://orcid.org/0000-0003-3202-9249	en_US
dc.contributor.author	Totsika, M	en_US
dc.contributor.author	Carey, AJ	en_US
dc.contributor.author	Stacey, KJ	en_US
dc.contributor.author	Hancock, V	en_US
dc.contributor.author	Saunders, BM https://orcid.org/0000-0003-0540-4445	en_US
dc.contributor.author	Ravasi, T	en_US
dc.contributor.author	Ulett, GC	en_US
dc.contributor.author	Schembri, MA	en_US
dc.contributor.author	Sweet, MJ	en_US
dc.date.available	2011-05-16	en_US
dc.date.issued	2011-11-01	en_US
dc.identifier.citation	Immunobiology, 2011, 216 (11), pp. 1164 - 1171	en_US
dc.identifier.issn	0171-2985	en_US
dc.identifier.uri	http://hdl.handle.net/10453/111319
dc.description.abstract	Uropathogenic E. coli (UPEC) are the primary cause of urinary tract infections. Recent studies have demonstrated that UPEC can invade and replicate within epithelial cells, suggesting that this bacterial pathogen may occupy an intracellular niche within the host. Given that many intracellular pathogens target macrophages, we assessed the interactions between UPEC and macrophages. Colonization of the mouse bladder by UPEC strain CFT073 resulted in increased expression of myeloid-restricted genes, consistent with the recruitment of inflammatory macrophages to the site of infection. In in vitro assays, CFT073 was able to survive within primary mouse bone marrow-derived macrophages (BMM) up to 24h post-infection. Three additional well-characterized clinical UPEC isolates associated with distinct UTI symptomatologies displayed variable long-term survival within BMM. UPEC strains UTI89 and VR50, originally isolated from patients with cystitis and asymptomatic bacteriuria respectively, showed elevated bacterial loads in BMM at 24h post-infection as compared to CFT073 and the asymptomatic bacteriuria strain 83972. These differences did not correlate with differential effects on macrophage survival or initial uptake of bacteria. E. coli UTI89 localized to a Lamp1 + vesicular compartment within BMM. In contrast to survival within mouse BMM, intracellular bacterial loads of VR50 were low in both human monocyte-derived macrophages (HMDM) and in human T24 bladder epithelial cells. Collectively, these data suggest that some UPEC isolates may subvert macrophage anti-microbial pathways, and that host species differences may impact on intracellular UPEC survival. © 2011 Elsevier GmbH.	en_US
dc.relation.ispartof	Immunobiology	en_US
dc.relation.isbasedon	10.1016/j.imbio.2011.05.011	en_US
dc.subject.classification	Immunology	en_US
dc.subject.mesh	Urothelium	en_US
dc.subject.mesh	Macrophages	en_US
dc.subject.mesh	Epithelial Cells	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice, Inbred C57BL	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Escherichia coli Infections	en_US
dc.subject.mesh	Urinary Tract Infections	en_US
dc.subject.mesh	Cystitis	en_US
dc.subject.mesh	Virulence Factors	en_US
dc.subject.mesh	Virulence	en_US
dc.subject.mesh	Species Specificity	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Urinary Bladder	en_US
dc.subject.mesh	Immunity, Innate	en_US
dc.subject.mesh	Uropathogenic Escherichia coli	en_US
dc.subject.mesh	Host Specificity	en_US
dc.title	Intramacrophage survival of uropathogenic Escherichia coli: Differences between diverse clinical isolates and between mouse and human macrophages	en_US
dc.type	Journal Article
utslib.citation.volume	11	en_US
utslib.citation.volume	216	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	11	en_US
pubs.publication-status	Published	en_US
pubs.volume	216	en_US

Abstract:

Uropathogenic E. coli (UPEC) are the primary cause of urinary tract infections. Recent studies have demonstrated that UPEC can invade and replicate within epithelial cells, suggesting that this bacterial pathogen may occupy an intracellular niche within the host. Given that many intracellular pathogens target macrophages, we assessed the interactions between UPEC and macrophages. Colonization of the mouse bladder by UPEC strain CFT073 resulted in increased expression of myeloid-restricted genes, consistent with the recruitment of inflammatory macrophages to the site of infection. In in vitro assays, CFT073 was able to survive within primary mouse bone marrow-derived macrophages (BMM) up to 24h post-infection. Three additional well-characterized clinical UPEC isolates associated with distinct UTI symptomatologies displayed variable long-term survival within BMM. UPEC strains UTI89 and VR50, originally isolated from patients with cystitis and asymptomatic bacteriuria respectively, showed elevated bacterial loads in BMM at 24h post-infection as compared to CFT073 and the asymptomatic bacteriuria strain 83972. These differences did not correlate with differential effects on macrophage survival or initial uptake of bacteria. E. coli UTI89 localized to a Lamp1 + vesicular compartment within BMM. In contrast to survival within mouse BMM, intracellular bacterial loads of VR50 were low in both human monocyte-derived macrophages (HMDM) and in human T24 bladder epithelial cells. Collectively, these data suggest that some UPEC isolates may subvert macrophage anti-microbial pathways, and that host species differences may impact on intracellular UPEC survival. © 2011 Elsevier GmbH.

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http://hdl.handle.net/10453/111319