Apoptosis is Induced in Chlamydia trachomatis-infected HEp-2 Cells by the Addition of a Combination Innate Immune Activation Compounds and the Inhibitor Wedelolactone

Huston, WM; Gloeckl, S; de Boer, L; Beagley, KW; Timms, P

Apoptosis is Induced in Chlamydia trachomatis-infected HEp-2 Cells by the Addition of a Combination Innate Immune Activation Compounds and the Inhibitor Wedelolactone

Huston, WM

Gloeckl, S de Boer, L Beagley, KW Timms, P

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Publication Type:: Journal Article
Citation:: American Journal of Reproductive Immunology, 2011, 65 (5), pp. 460 - 465
Issue Date:: 2011-05-01

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Field	Value	Language
dc.contributor.author	Huston, WM https://orcid.org/0000-0002-0879-1287	en_US
dc.contributor.author	Gloeckl, S	en_US
dc.contributor.author	de Boer, L	en_US
dc.contributor.author	Beagley, KW	en_US
dc.contributor.author	Timms, P	en_US
dc.date.issued	2011-05-01	en_US
dc.identifier.citation	American Journal of Reproductive Immunology, 2011, 65 (5), pp. 460 - 465	en_US
dc.identifier.issn	1046-7408	en_US
dc.identifier.uri	http://hdl.handle.net/10453/111505
dc.description.abstract	Problem Innate immune activation of human cells, for some intracellular pathogens, is advantageous for vacuole morphology and pathogenic viability. It is unknown whether innate immune activation is advantageous to Chlamydia trachomatis viability. Method of study Innate immune activation of HEp-2 cells during Chlamydia infection was conducted using lipopolysaccharide (LPS), polyI:C, and wedelolactone (innate immune inhibitor) to investigate the impact of these conditions on viability of Chlamydia. Results The addition of LPS and polyI:C to stimulate activation of the two distinct innate immune pathways (nuclear factor kappa beta and interferon regulatory factor) had no impact on the viability of Chlamydia. However, when compounds targeting either pathway were added in combination with the specific innate immune inhibitor (wedelolactone) a major impact on Chlamydia viability was observed. This impact was found to be due to the induction of apoptosis of the HEp-2 cells under these conditions. Conclusion This is the first time that induction of apoptosis has been reported in C. trachomatis-infected cells when treated with a combination of innate immune activators and wedelolactone. © 2010 John Wiley and Sons A/S.	en_US
dc.relation.ispartof	American Journal of Reproductive Immunology	en_US
dc.relation.isbasedon	10.1111/j.1600-0897.2010.00936.x	en_US
dc.subject.classification	Obstetrics & Reproductive Medicine	en_US
dc.subject.mesh	Cell Line	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Chlamydia trachomatis	en_US
dc.subject.mesh	Chlamydia Infections	en_US
dc.subject.mesh	Coumarins	en_US
dc.subject.mesh	Lipopolysaccharides	en_US
dc.subject.mesh	NF-kappa B	en_US
dc.subject.mesh	Apoptosis	en_US
dc.subject.mesh	Gene Expression Regulation	en_US
dc.subject.mesh	Microbial Viability	en_US
dc.subject.mesh	Interferon Regulatory Factors	en_US
dc.subject.mesh	I-kappa B Kinase	en_US
dc.subject.mesh	Immunity, Innate	en_US
dc.title	Apoptosis is Induced in Chlamydia trachomatis-infected HEp-2 Cells by the Addition of a Combination Innate Immune Activation Compounds and the Inhibitor Wedelolactone	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	65	en_US
utslib.for	1103 Clinical Sciences	en_US
utslib.for	1107 Immunology	en_US
utslib.for	1114 Paediatrics and Reproductive Medicine	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	65	en_US

Abstract:

Problem Innate immune activation of human cells, for some intracellular pathogens, is advantageous for vacuole morphology and pathogenic viability. It is unknown whether innate immune activation is advantageous to Chlamydia trachomatis viability. Method of study Innate immune activation of HEp-2 cells during Chlamydia infection was conducted using lipopolysaccharide (LPS), polyI:C, and wedelolactone (innate immune inhibitor) to investigate the impact of these conditions on viability of Chlamydia. Results The addition of LPS and polyI:C to stimulate activation of the two distinct innate immune pathways (nuclear factor kappa beta and interferon regulatory factor) had no impact on the viability of Chlamydia. However, when compounds targeting either pathway were added in combination with the specific innate immune inhibitor (wedelolactone) a major impact on Chlamydia viability was observed. This impact was found to be due to the induction of apoptosis of the HEp-2 cells under these conditions. Conclusion This is the first time that induction of apoptosis has been reported in C. trachomatis-infected cells when treated with a combination of innate immune activators and wedelolactone. © 2010 John Wiley and Sons A/S.

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http://hdl.handle.net/10453/111505