Clostridium chauvoei, an evolutionary dead-end pathogen

Rychener, L; Albon, SI; Djordjevic, SP; Chowdhury, PR; Ziech, RE; de Vargas, AC; Frey, J; Falquet, L

Clostridium chauvoei, an evolutionary dead-end pathogen

Rychener, L Albon, SI Djordjevic, SP

Chowdhury, PR

Ziech, RE de Vargas, AC Frey, J Falquet, L

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Publication Type:: Journal Article
Citation:: Frontiers in Microbiology, 2017, 8 (JUN)
Issue Date:: 2017-06-09

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Field	Value	Language
dc.contributor.author	Rychener, L	en_US
dc.contributor.author	Albon, SI	en_US
dc.contributor.author	Djordjevic, SP https://orcid.org/0000-0001-9301-5372	en_US
dc.contributor.author	Chowdhury, PR https://orcid.org/0000-0003-4352-603X	en_US
dc.contributor.author	Ziech, RE	en_US
dc.contributor.author	de Vargas, AC	en_US
dc.contributor.author	Frey, J	en_US
dc.contributor.author	Falquet, L	en_US
dc.date.available	2017-05-26	en_US
dc.date.issued	2017-06-09	en_US
dc.identifier.citation	Frontiers in Microbiology, 2017, 8 (JUN)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/111703
dc.description.abstract	© 2017 Rychener, InAlbon, Djordjevic, Chowdhury, Ziech, de Vargas, Frey and Falquet. Full genome sequences of 20 strains of Clostridium chauvoei, the etiological agent of blackleg of cattle and sheep, isolated from four different continents over a period of 64 years (1951-2015) were determined and analyzed. The study reveals that the genome of the species C. chauvoei is highly homogeneous compared to the closely related species C. perfringens, a widespread pathogen that affects human and many animal species. Analysis of the CRISPR locus is sufficient to differentiate most C. chauvoei strains and is the most heterogenous region in the genome, containing in total 187 different spacer elements that are distributed as 30 - 77 copies in the various strains. Some genetic differences are found in the 3 allelic variants of fliC1, fliC2 and fliC3 genes that encode structural flagellin proteins, and certain strains do only contain one or two alleles. However, the major virulence genes including the highly toxic C. chauvoei toxin A, the sialidase and the two hyaluronidases are fully conserved as are the metabolic and structural genes of C. chauvoei. These data indicate that C. chauvoei is a strict ruminant-associated pathogen that has reached a dead end in its evolution.	en_US
dc.relation.ispartof	Frontiers in Microbiology	en_US
dc.relation.isbasedon	10.3389/fmicb.2017.01054	en_US
dc.title	Clostridium chauvoei, an evolutionary dead-end pathogen	en_US
dc.type	Journal Article
utslib.citation.volume	JUN	en_US
utslib.citation.volume	8	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0502 Environmental Science and Management	en_US
utslib.for	0503 Soil Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	JUN	en_US
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

© 2017 Rychener, InAlbon, Djordjevic, Chowdhury, Ziech, de Vargas, Frey and Falquet. Full genome sequences of 20 strains of Clostridium chauvoei, the etiological agent of blackleg of cattle and sheep, isolated from four different continents over a period of 64 years (1951-2015) were determined and analyzed. The study reveals that the genome of the species C. chauvoei is highly homogeneous compared to the closely related species C. perfringens, a widespread pathogen that affects human and many animal species. Analysis of the CRISPR locus is sufficient to differentiate most C. chauvoei strains and is the most heterogenous region in the genome, containing in total 187 different spacer elements that are distributed as 30 - 77 copies in the various strains. Some genetic differences are found in the 3 allelic variants of fliC1, fliC2 and fliC3 genes that encode structural flagellin proteins, and certain strains do only contain one or two alleles. However, the major virulence genes including the highly toxic C. chauvoei toxin A, the sialidase and the two hyaluronidases are fully conserved as are the metabolic and structural genes of C. chauvoei. These data indicate that C. chauvoei is a strict ruminant-associated pathogen that has reached a dead end in its evolution.

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http://hdl.handle.net/10453/111703