A metaproteomic assessment of winter and summer bacterioplankton from Antarctic Peninsula coastal surface waters

Williams, TJ; Long, E; Evans, F; Demaere, MZ; Lauro, FM; Raftery, MJ; Ducklow, H; Grzymski, JJ; Murray, AE; Cavicchioli, R

A metaproteomic assessment of winter and summer bacterioplankton from Antarctic Peninsula coastal surface waters

Williams, TJ Long, E Evans, F Demaere, MZ

Lauro, FM Raftery, MJ Ducklow, H Grzymski, JJ Murray, AE Cavicchioli, R

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Publication Type:: Journal Article
Citation:: ISME Journal, 2012, 6 (10), pp. 1883 - 1900
Issue Date:: 2012-10-01

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Field	Value	Language
dc.contributor.author	Williams, TJ	en_US
dc.contributor.author	Long, E	en_US
dc.contributor.author	Evans, F	en_US
dc.contributor.author	Demaere, MZ https://orcid.org/0000-0002-7601-5108	en_US
dc.contributor.author	Lauro, FM	en_US
dc.contributor.author	Raftery, MJ	en_US
dc.contributor.author	Ducklow, H	en_US
dc.contributor.author	Grzymski, JJ	en_US
dc.contributor.author	Murray, AE	en_US
dc.contributor.author	Cavicchioli, R	en_US
dc.date.issued	2012-10-01	en_US
dc.identifier.citation	ISME Journal, 2012, 6 (10), pp. 1883 - 1900	en_US
dc.identifier.issn	1751-7362	en_US
dc.identifier.uri	http://hdl.handle.net/10453/113629
dc.description.abstract	A metaproteomic survey of surface coastal waters near Palmer Station on the Antarctic Peninsula, West Antarctica, was performed, revealing marked differences in the functional capacity of summer and winter communities of bacterioplankton. Proteins from Flavobacteria were more abundant in the summer metaproteome, whereas winter was characterized by proteins from ammonia-oxidizing Marine Group I Crenarchaeota. Proteins prevalent in both seasons were from SAR11 and Rhodobacterales clades of Alphaproteobacteria, as well as many lineages of Gammaproteobacteria. The metaproteome data were used to elucidate the main metabolic and energy generation pathways and transport processes occurring at the microbial level in each season. In summer, autotrophic carbon assimilation appears to be driven by oxygenic photoautotrophy, consistent with high light availability and intensity. In contrast, during the dark polar winter, the metaproteome supported the occurrence of chemolithoautotrophy via the 3-hydroxypropionate/4-hydroxybutyrate cycle and the reverse tricarboxylic acid cycle of ammonia-oxidizing archaea and nitrite-oxidizing bacteria, respectively. Proteins involved in nitrification were also detected in the metaproteome. Taurine appears to be an important source of carbon and nitrogen for heterotrophs (especially SAR11), with transporters and enzymes for taurine uptake and degradation abundant in the metaproteome. Divergent heterotrophic strategies for Alphaproteobacteria and Flavobacteria were indicated by the metaproteome data, with Alphaproteobacteria capturing (by high-affinity transport) and processing labile solutes, and Flavobacteria expressing outer membrane receptors for particle adhesion to facilitate the exploitation of non-labile substrates. TonB-dependent receptors from Gammaproteobacteria and Flavobacteria (particularly in summer) were abundant, indicating that scavenging of substrates was likely an important strategy for these clades of Southern Ocean bacteria. This study provides the first insight into differences in functional processes occurring between summer and winter microbial communities in coastal Antarctic waters, and particularly highlights the important role that dark carbon fixation has in winter. © 2012 International Society for Microbial Ecology. All rights reserved.	en_US
dc.relation.ispartof	ISME Journal	en_US
dc.relation.isbasedon	10.1038/ismej.2012.28	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Plankton	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	Crenarchaeota	en_US
dc.subject.mesh	Ammonia	en_US
dc.subject.mesh	Water	en_US
dc.subject.mesh	Proteome	en_US
dc.subject.mesh	Seasons	en_US
dc.subject.mesh	Seawater	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Antarctic Regions	en_US
dc.subject.mesh	Oceans and Seas	en_US
dc.subject.mesh	Heterotrophic Processes	en_US
dc.subject.mesh	Nitrification	en_US
dc.title	A metaproteomic assessment of winter and summer bacterioplankton from Antarctic Peninsula coastal surface waters	en_US
dc.type	Journal Article
utslib.citation.volume	10	en_US
utslib.citation.volume	6	en_US
utslib.for	0602 Ecology	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	10 Technology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	10	en_US
pubs.publication-status	Published	en_US
pubs.volume	6	en_US

Abstract:

A metaproteomic survey of surface coastal waters near Palmer Station on the Antarctic Peninsula, West Antarctica, was performed, revealing marked differences in the functional capacity of summer and winter communities of bacterioplankton. Proteins from Flavobacteria were more abundant in the summer metaproteome, whereas winter was characterized by proteins from ammonia-oxidizing Marine Group I Crenarchaeota. Proteins prevalent in both seasons were from SAR11 and Rhodobacterales clades of Alphaproteobacteria, as well as many lineages of Gammaproteobacteria. The metaproteome data were used to elucidate the main metabolic and energy generation pathways and transport processes occurring at the microbial level in each season. In summer, autotrophic carbon assimilation appears to be driven by oxygenic photoautotrophy, consistent with high light availability and intensity. In contrast, during the dark polar winter, the metaproteome supported the occurrence of chemolithoautotrophy via the 3-hydroxypropionate/4-hydroxybutyrate cycle and the reverse tricarboxylic acid cycle of ammonia-oxidizing archaea and nitrite-oxidizing bacteria, respectively. Proteins involved in nitrification were also detected in the metaproteome. Taurine appears to be an important source of carbon and nitrogen for heterotrophs (especially SAR11), with transporters and enzymes for taurine uptake and degradation abundant in the metaproteome. Divergent heterotrophic strategies for Alphaproteobacteria and Flavobacteria were indicated by the metaproteome data, with Alphaproteobacteria capturing (by high-affinity transport) and processing labile solutes, and Flavobacteria expressing outer membrane receptors for particle adhesion to facilitate the exploitation of non-labile substrates. TonB-dependent receptors from Gammaproteobacteria and Flavobacteria (particularly in summer) were abundant, indicating that scavenging of substrates was likely an important strategy for these clades of Southern Ocean bacteria. This study provides the first insight into differences in functional processes occurring between summer and winter microbial communities in coastal Antarctic waters, and particularly highlights the important role that dark carbon fixation has in winter. © 2012 International Society for Microbial Ecology. All rights reserved.

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http://hdl.handle.net/10453/113629