Detection of kdr pyrethroid resistance in the cotton aphid, Aphis gossypii (Hemiptera: Aphididae), using a PCR-RFLP assay

Pesticide Science Society of Japan
Publication Type:
Journal Article
Journal of Pesticide Science, 2012, 37 (2), pp. 169 - 172
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Two point mutations, Leu1014 to Phe and Met918 to r, located in the domain II region of the para-type voltage gated sodium channel, confer knockdown resistance (kdr) to pyrethroid insecticides in several insect species. PCR ampli cation of this region in a pyrethroid-resistant and pyrethroid-susceptible strain of Aphis gossypii and use of a restriction fragment length polymorphism assay on PCR products (PCR-RFLP) provide the rst direct evidence of the Leu1014 to Phe kdr mutation in a eld isolate of A. gossypii.
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