Laser oblique scanning optical microscopy (LOSOM) for phase relief imaging
- Publication Type:
- Journal Article
- Optics Express, 2012, 20 (13), pp. 14100 - 14108
- Issue Date:
The visualization of optical phase can provide abundant information when imaging transparent specimen. We present a novel phase sensitive imaging design capable of obtaining phase contours of transparent biological cells through laser oblique scanning optical microscope (LOSOM). LOSOM is based on the introduction of a fluorescent medium behind the specimen to generate a differential phase-sensitive image, thus, the complicated phase retardation alignment procedure associated with differential interference contrast (DIC) microscopy can be eliminated. Moreover, multi-modality fluorescence and phase relief imaging can be attained in a single system with fluorescently labeled specimens. © 2012 Optical Society of America.
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