Developing red-emissive ruthenium(II) complex-based luminescent probes for cellular imaging

Publication Type:
Journal Article
Bioconjugate Chemistry, 2012, 23 (4), pp. 725 - 733
Issue Date:
Filename Description Size
bc200506w.pdfPublished Version2.09 MB
Adobe PDF
Full metadata record
(Figure Presented) Ruthenium(II) complexes have rich photophysical attributes, which enable novel design of responsive luminescence probes to selectively quantify biochemical analytes. In this work, we developed a systematic series of Ru(II)- bipyrindine complex derivatives, [Ru(bpy) 3-n(DNP-bpy) n](PF 6) 2 (n = 1, 2, 3; bpy, 2,2′-bipyridine; DNP-bpy, 4-(4-(2,4- dinitrophenoxy)phenyl)-2, 2′-bipyridine), as luminescent probes for highly selective and sensitive detection of thiophenol in aqueous solutions. The specific reaction between the probes and thiophenol triggers the cleavage of the electron acceptor group, 2,4-dinitrophenyl, eliminating the photoinduced electron transfer (PET) process, so that the luminescence of on-state complexes, [Ru(bpy) 3-n(HP-bpy) n] 2+ (n = 1, 2, 3; HP-bpy, 4-(4-hydroxyphenyl)-2,2′- bipyridine), is turned on. We found that the complex [Ru(bpy)(DNP-bpy) 2] 2+ remarkably enhanced the on-to-off contrast ratio compared to the other two (37.8 compared to 21 and 18.7). This reveals a new strategy to obtain the best Ru(II) complex luminescence probe via the most asymmetric structure. Moreover, we demonstrated the practical utility of the complex as a cell-membrane permeable probe for quantitative luminescence imaging of the dynamic intracellular process of thiophenol in living cells. The results suggest that the new probe could be a very useful tool for luminescence imaging analysis of the toxic thiophenol in intact cells. © 2012 American Chemical Society.
Please use this identifier to cite or link to this item: