Role of Modular Polyketide Synthases in the Production of Polyether Ladder Compounds in Ciguatoxin-Producing Gambierdiscus polynesiensis and G. excentricus (Dinophyceae)

Kohli, GS; Campbell, K; John, U; Smith, KF; Fraga, S; Rhodes, LL; Murray, SA

Role of Modular Polyketide Synthases in the Production of Polyether Ladder Compounds in Ciguatoxin-Producing Gambierdiscus polynesiensis and G. excentricus (Dinophyceae)

Kohli, GS

Campbell, K John, U Smith, KF Fraga, S Rhodes, LL Murray, SA

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Publication Type:: Journal Article
Citation:: Journal of Eukaryotic Microbiology, 2017, 64 (5), pp. 691 - 706
Issue Date:: 2017-09-01

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Field	Value	Language
dc.contributor.author	Kohli, GS https://orcid.org/0000-0002-4578-2355	en_US
dc.contributor.author	Campbell, K	en_US
dc.contributor.author	John, U	en_US
dc.contributor.author	Smith, KF	en_US
dc.contributor.author	Fraga, S	en_US
dc.contributor.author	Rhodes, LL	en_US
dc.contributor.author	Murray, SA https://orcid.org/0000-0001-7096-1307	en_US
dc.date.available	2017-02-03	en_US
dc.date.issued	2017-09-01	en_US
dc.identifier.citation	Journal of Eukaryotic Microbiology, 2017, 64 (5), pp. 691 - 706	en_US
dc.identifier.issn	1066-5234	en_US
dc.identifier.uri	http://hdl.handle.net/10453/115016
dc.description.abstract	© 2017 The Author(s) Journal of Eukaryotic Microbiology © 2017 International Society of Protistologists Gambierdiscus, a benthic dinoflagellate, produces ciguatoxins that cause the human illness Ciguatera. Ciguatoxins are polyether ladder compounds that have a polyketide origin, indicating that polyketide synthases (PKS) are involved in their production. We sequenced transcriptomes of Gambierdiscus excentricus and Gambierdiscus polynesiensis and found 264 contigs encoding single domain ketoacyl synthases (KS; G. excentricus: 106, G. polynesiensis: 143) and ketoreductases (KR; G. excentricus: 7, G. polynesiensis: 8) with sequence similarity to type I PKSs, as reported in other dinoflagellates. In addition, 24 contigs (G. excentricus: 3, G. polynesiensis: 21) encoding multiple PKS domains (forming typical type I PKSs modules) were found. The proposed structure produced by one of these megasynthases resembles a partial carbon backbone of a polyether ladder compound. Seventeen contigs encoding single domain KS, KR, s-malonyltransacylase, dehydratase and enoyl reductase with sequence similarity to type II fatty acid synthases (FAS) in plants were found. Type I PKS and type II FAS genes were distinguished based on the arrangement of domains on the contigs and their sequence similarity and phylogenetic clustering with known PKS/FAS genes in other organisms. This differentiation of PKS and FAS pathways in Gambierdiscus is important, as it will facilitate approaches to investigating toxin biosynthesis pathways in dinoflagellates.	en_US
dc.relation	http://purl.org/au-research/grants/arc/FT120100704
dc.relation.ispartof	Journal of Eukaryotic Microbiology	en_US
dc.relation.isbasedon	10.1111/jeu.12405	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Dinoflagellida	en_US
dc.subject.mesh	Ciguatoxins	en_US
dc.subject.mesh	Polyketide Synthases	en_US
dc.subject.mesh	Fatty Acids	en_US
dc.subject.mesh	Protozoan Proteins	en_US
dc.subject.mesh	Gene Expression Profiling	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Gene Expression Regulation, Enzymologic	en_US
dc.subject.mesh	Amino Acid Sequence	en_US
dc.subject.mesh	Sequence Homology, Amino Acid	en_US
dc.subject.mesh	Models, Molecular	en_US
dc.subject.mesh	Biosynthetic Pathways	en_US
dc.subject.mesh	Fatty Acid Synthases	en_US
dc.title	Role of Modular Polyketide Synthases in the Production of Polyether Ladder Compounds in Ciguatoxin-Producing Gambierdiscus polynesiensis and G. excentricus (Dinophyceae)	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	64	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0608 Zoology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	64	en_US

Abstract:

© 2017 The Author(s) Journal of Eukaryotic Microbiology © 2017 International Society of Protistologists Gambierdiscus, a benthic dinoflagellate, produces ciguatoxins that cause the human illness Ciguatera. Ciguatoxins are polyether ladder compounds that have a polyketide origin, indicating that polyketide synthases (PKS) are involved in their production. We sequenced transcriptomes of Gambierdiscus excentricus and Gambierdiscus polynesiensis and found 264 contigs encoding single domain ketoacyl synthases (KS; G. excentricus: 106, G. polynesiensis: 143) and ketoreductases (KR; G. excentricus: 7, G. polynesiensis: 8) with sequence similarity to type I PKSs, as reported in other dinoflagellates. In addition, 24 contigs (G. excentricus: 3, G. polynesiensis: 21) encoding multiple PKS domains (forming typical type I PKSs modules) were found. The proposed structure produced by one of these megasynthases resembles a partial carbon backbone of a polyether ladder compound. Seventeen contigs encoding single domain KS, KR, s-malonyltransacylase, dehydratase and enoyl reductase with sequence similarity to type II fatty acid synthases (FAS) in plants were found. Type I PKS and type II FAS genes were distinguished based on the arrangement of domains on the contigs and their sequence similarity and phylogenetic clustering with known PKS/FAS genes in other organisms. This differentiation of PKS and FAS pathways in Gambierdiscus is important, as it will facilitate approaches to investigating toxin biosynthesis pathways in dinoflagellates.

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http://hdl.handle.net/10453/115016