Infrared spectroscopic characterization of monocytic microvesicles (microparticles) released upon lipopolysaccharide stimulation

Lee, J; Wen, B; Carter, EA; Combes, V; Grau, GER; Lay, PA

Infrared spectroscopic characterization of monocytic microvesicles (microparticles) released upon lipopolysaccharide stimulation

Lee, J Wen, B Carter, EA Combes, V

Grau, GER Lay, PA

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Publication Type:: Journal Article
Citation:: FASEB Journal, 2017, 31 (7), pp. 2817 - 2827
Issue Date:: 2017-07-01

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	FASEB J-2017-Lee-fj.201601272R.pdf	Published Version	1.84 MB	Adobe PDF	View/Open
	MP FTIR FASEB J revised.docx	Accepted Manuscript Version	1.71 MB	Microsoft Word XML	View/Open

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Field	Value	Language
dc.contributor.author	Lee, J	en_US
dc.contributor.author	Wen, B	en_US
dc.contributor.author	Carter, EA	en_US
dc.contributor.author	Combes, V https://orcid.org/0000-0003-2178-3596	en_US
dc.contributor.author	Grau, GER	en_US
dc.contributor.author	Lay, PA	en_US
dc.date.available	2017-02-26	en_US
dc.date.issued	2017-07-01	en_US
dc.identifier.citation	FASEB Journal, 2017, 31 (7), pp. 2817 - 2827	en_US
dc.identifier.issn	0892-6638	en_US
dc.identifier.uri	http://hdl.handle.net/10453/115044
dc.description.abstract	© FASEB. Microvesicles (MVs) are involved in cell-cell interactions, including disease pathogenesis. Nondestructive Fourier-transform infrared (FTIR) spectra from MVs were assessed as a technique to provide new biochemical insights into a LPS-induced monocyte model of septic shock. FTIR spectroscopy provided a quick method to investigate relative differences in biomolecular content of different MV populations that was complementary to traditional semiquantitative omics approaches, with which it is difficult to provide information on relative changes between classes (proteins, lipids, nucleic acids, carbohydrates) or protein conformations. Timedependent changes were detected in biomolecular contents of MVs and in the monocytes from which they were released. Differences in phosphatidylcholine and phosphatidylserine contents were observed in MVs released under stimulation, and higher relative concentrations of RNA and a-helical structured proteins were present in stimulatedMVs compared withMVs fromresting cells. FTIR spectra of stimulated monocytes displayed changes that were consistent with those observed in the correspondingMVs they released. LPS-stimulated monocytes had reduced concentrations of nucleic acids, a-helical structured proteins, and phosphatidylcholine compared with restingmonocytes but had an increase in total lipids. FTIRspectra ofMVbiomolecular content will be important in shedding new light on the mechanisms of MVs and the different roles they play in physiology and disease pathogenesis.	en_US
dc.relation.ispartof	FASEB Journal	en_US
dc.relation.isbasedon	10.1096/fj.201601272R	en_US
dc.subject.classification	Biochemistry & Molecular Biology	en_US
dc.subject.mesh	Monocytes	en_US
dc.subject.mesh	Cell Line	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Lipopolysaccharides	en_US
dc.subject.mesh	Flow Cytometry	en_US
dc.subject.mesh	Spectroscopy, Fourier Transform Infrared	en_US
dc.subject.mesh	Cell-Derived Microparticles	en_US
dc.title	Infrared spectroscopic characterization of monocytic microvesicles (microparticles) released upon lipopolysaccharide stimulation	en_US
dc.type	Journal Article
utslib.citation.volume	7	en_US
utslib.citation.volume	31	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	0606 Physiology	en_US
utslib.for	1116 Medical Physiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	7	en_US
pubs.publication-status	Published	en_US
pubs.volume	31	en_US

Abstract:

© FASEB. Microvesicles (MVs) are involved in cell-cell interactions, including disease pathogenesis. Nondestructive Fourier-transform infrared (FTIR) spectra from MVs were assessed as a technique to provide new biochemical insights into a LPS-induced monocyte model of septic shock. FTIR spectroscopy provided a quick method to investigate relative differences in biomolecular content of different MV populations that was complementary to traditional semiquantitative omics approaches, with which it is difficult to provide information on relative changes between classes (proteins, lipids, nucleic acids, carbohydrates) or protein conformations. Timedependent changes were detected in biomolecular contents of MVs and in the monocytes from which they were released. Differences in phosphatidylcholine and phosphatidylserine contents were observed in MVs released under stimulation, and higher relative concentrations of RNA and a-helical structured proteins were present in stimulatedMVs compared withMVs fromresting cells. FTIR spectra of stimulated monocytes displayed changes that were consistent with those observed in the correspondingMVs they released. LPS-stimulated monocytes had reduced concentrations of nucleic acids, a-helical structured proteins, and phosphatidylcholine compared with restingmonocytes but had an increase in total lipids. FTIRspectra ofMVbiomolecular content will be important in shedding new light on the mechanisms of MVs and the different roles they play in physiology and disease pathogenesis.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/115044