Large-scale growth of the Plasmodium falciparum malaria parasite in a wave bioreactor.

Publication Type:
Journal Article
Citation:
International journal for parasitology, 2012, 42 (3), pp. 215 - 220
Issue Date:
2012-01
Full metadata record
Files in This Item:
Filename Description Size
1-s2.0-S0020751912000185-main.pdfPublished Version1.41 MB
Adobe PDF
We describe methods for the large-scale in vitro culturing of synchronous and asynchronous blood-stage Plasmodium falciparum parasites in sterile disposable plastic bioreactors controlled by wave-induced motion (wave bioreactor). These cultures perform better than static flask cultures in terms of preserving parasite cell cycle synchronicity and reducing the number of multiple-infected erythrocytes. The straight-forward methods described here will facilitate the large scale production of malaria parasites for antigen and organelle isolation and characterisation, for the high throughput screening of compound libraries with whole cells or extracts, and the development of live- or whole-cell malaria vaccines under good manufacturing practice compliant standards.
Please use this identifier to cite or link to this item: