Large-scale growth of the Plasmodium falciparum malaria parasite in a wave bioreactor

Dalton, JP; Demanga, CG; Reiling, SJ; Wunderlich, J; Eng, JWL; Rohrbach, P

Large-scale growth of the Plasmodium falciparum malaria parasite in a wave bioreactor

Dalton, JP Demanga, CG Reiling, SJ Wunderlich, J Eng, JWL Rohrbach, P

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Publication Type:: Journal Article
Citation:: International Journal for Parasitology, 2012, 42 (3), pp. 215 - 220
Issue Date:: 2012-03-01

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Field	Value	Language
dc.contributor.author	Dalton, JP	en_US
dc.contributor.author	Demanga, CG	en_US
dc.contributor.author	Reiling, SJ	en_US
dc.contributor.author	Wunderlich, J	en_US
dc.contributor.author	Eng, JWL	en_US
dc.contributor.author	Rohrbach, P	en_US
dc.date.available	2012-01-23	en_US
dc.date.issued	2012-03-01	en_US
dc.identifier.citation	International Journal for Parasitology, 2012, 42 (3), pp. 215 - 220	en_US
dc.identifier.issn	0020-7519	en_US
dc.identifier.uri	http://hdl.handle.net/10453/115110
dc.description.abstract	We describe methods for the large-scale in vitro culturing of synchronous and asynchronous blood-stage Plasmodium falciparum parasites in sterile disposable plastic bioreactors controlled by wave-induced motion (wave bioreactor). These cultures perform better than static flask cultures in terms of preserving parasite cell cycle synchronicity and reducing the number of multiple-infected erythrocytes. The straight-forward methods described here will facilitate the large scale production of malaria parasites for antigen and organelle isolation and characterisation, for the high throughput screening of compound libraries with whole cells or extracts, and the development of live- or whole-cell malaria vaccines under good manufacturing practice compliant standards. © 2012.	en_US
dc.relation.ispartof	International Journal for Parasitology	en_US
dc.relation.isbasedon	10.1016/j.ijpara.2012.01.001	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Plasmodium falciparum	en_US
dc.subject.mesh	Malaria, Falciparum	en_US
dc.subject.mesh	Cell Culture Techniques	en_US
dc.subject.mesh	Bioreactors	en_US
dc.title	Large-scale growth of the Plasmodium falciparum malaria parasite in a wave bioreactor	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	42	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0608 Zoology	en_US
utslib.for	0707 Veterinary Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	42	en_US

Abstract:

We describe methods for the large-scale in vitro culturing of synchronous and asynchronous blood-stage Plasmodium falciparum parasites in sterile disposable plastic bioreactors controlled by wave-induced motion (wave bioreactor). These cultures perform better than static flask cultures in terms of preserving parasite cell cycle synchronicity and reducing the number of multiple-infected erythrocytes. The straight-forward methods described here will facilitate the large scale production of malaria parasites for antigen and organelle isolation and characterisation, for the high throughput screening of compound libraries with whole cells or extracts, and the development of live- or whole-cell malaria vaccines under good manufacturing practice compliant standards. © 2012.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/115110