Bacterial membrane vesicles transport their DNA cargo into host cells

Bitto, NJ; Chapman, R; Pidot, S; Costin, A; Lo, C; Choi, J; D'Cruze, T; Reynolds, EC; Dashper, SG; Turnbull, L; Whitchurch, CB; Stinear, TP; Stacey, KJ; Ferrero, RL

Bacterial membrane vesicles transport their DNA cargo into host cells

Bitto, NJ Chapman, R Pidot, S Costin, A Lo, C Choi, J D'Cruze, T Reynolds, EC Dashper, SG Turnbull, L

Whitchurch, CB

Stinear, TP Stacey, KJ Ferrero, RL

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Publication Type:: Journal Article
Citation:: Scientific Reports, 2017, 7 (1)
Issue Date:: 2017-12-01

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Field	Value	Language
dc.contributor.author	Bitto, NJ	en_US
dc.contributor.author	Chapman, R	en_US
dc.contributor.author	Pidot, S	en_US
dc.contributor.author	Costin, A	en_US
dc.contributor.author	Lo, C	en_US
dc.contributor.author	Choi, J	en_US
dc.contributor.author	D'Cruze, T	en_US
dc.contributor.author	Reynolds, EC	en_US
dc.contributor.author	Dashper, SG	en_US
dc.contributor.author	Turnbull, L https://orcid.org/0000-0002-9255-9033	en_US
dc.contributor.author	Whitchurch, CB https://orcid.org/0000-0003-2296-3791	en_US
dc.contributor.author	Stinear, TP	en_US
dc.contributor.author	Stacey, KJ	en_US
dc.contributor.author	Ferrero, RL	en_US
dc.date.available	2017-06-27	en_US
dc.date.issued	2017-12-01	en_US
dc.identifier.citation	Scientific Reports, 2017, 7 (1)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/115196
dc.description.abstract	© 2017 The Author(s). Bacterial outer membrane vesicles (OMVs) are extracellular sacs containing biologically active products, such as proteins, cell wall components and toxins. OMVs are reported to contain DNA, however, little is known about the nature of this DNA, nor whether it can be transported into host cells. Our work demonstrates that chromosomal DNA is packaged into OMVs shed by bacteria during exponential phase. Most of this DNA was present on the external surfaces of OMVs, with smaller amounts located internally. The DNA within the internal compartments of Pseudomonas aeruginosa OMVs were consistently enriched in specific regions of the bacterial chromosome, encoding proteins involved in virulence, stress response, antibiotic resistance and metabolism. Furthermore, we demonstrated that OMVs carry DNA into eukaryotic cells, and this DNA was detectable by PCR in the nuclear fraction of cells. These findings suggest a role for OMV-associated DNA in bacterial-host cell interactions and have implications for OMV-based vaccines.	en_US
dc.relation.ispartof	Scientific Reports	en_US
dc.relation.isbasedon	10.1038/s41598-017-07288-4	en_US
dc.subject.mesh	Cell Line	en_US
dc.subject.mesh	Epithelial Cells	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Gram-Negative Bacteria	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	Endocytosis	en_US
dc.subject.mesh	Extracellular Vesicles	en_US
dc.title	Bacterial membrane vesicles transport their DNA cargo into host cells	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	7	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	7	en_US

Abstract:

© 2017 The Author(s). Bacterial outer membrane vesicles (OMVs) are extracellular sacs containing biologically active products, such as proteins, cell wall components and toxins. OMVs are reported to contain DNA, however, little is known about the nature of this DNA, nor whether it can be transported into host cells. Our work demonstrates that chromosomal DNA is packaged into OMVs shed by bacteria during exponential phase. Most of this DNA was present on the external surfaces of OMVs, with smaller amounts located internally. The DNA within the internal compartments of Pseudomonas aeruginosa OMVs were consistently enriched in specific regions of the bacterial chromosome, encoding proteins involved in virulence, stress response, antibiotic resistance and metabolism. Furthermore, we demonstrated that OMVs carry DNA into eukaryotic cells, and this DNA was detectable by PCR in the nuclear fraction of cells. These findings suggest a role for OMV-associated DNA in bacterial-host cell interactions and have implications for OMV-based vaccines.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/115196