Flow cytometric analysis of microparticles.

Publication Type:
Journal Article
Citation:
Methods in molecular biology (Clifton, N.J.), 2011, 699 pp. 337 - 354
Issue Date:
2011-01
Full metadata record
Files in This Item:
Filename Description Size
FCM analysis of MP van der Heyde et al Meth Mol Biol 2011.pdfPublished Version1.85 MB
Adobe PDF
Cell-derived microparticles (MPs) are increasingly recognized as important cell-to-cell signaling mechanisms and may exhibit important functions in homeostasis but also in pathogenesis. Indeed, MPs are associated with a number of diseases inhibiting their production that protects against pathogenesis. MPs are distinct from exosomes and apoptotic bodies, often exhibiting the membrane proteins of the activated or apoptotic cell from which they are derived. Electron microscopic analyses have shown that MPs are produced by all cell types tested to date, and ELISA-based assays have established that increased numbers of MPs are produced following cell activation. These approaches do not, however, determine the exact number of MPs and distribution of functional proteins on their surface. Flow cytometry represents an obvious approach to analyze MPs, and we present here a method to assess the number and phenotype of MPs by using a conventional flow cytometer. We also present the caveats with this method and describe a new imaging flow cytometry approach that overcomes these limitations.
Please use this identifier to cite or link to this item: