Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi

Williams, TJ; Liao, Y; Ye, J; Kuchel, RP; Poljak, A; Raftery, MJ; Cavicchioli, R

Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi

Williams, TJ Liao, Y

Ye, J

Kuchel, RP Poljak, A Raftery, MJ Cavicchioli, R

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Publication Type:: Journal Article
Citation:: Environmental Microbiology, 2017, 19 (6), pp. 2210 - 2227
Issue Date:: 2017-06-01

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Field	Value	Language
dc.contributor.author	Williams, TJ	en_US
dc.contributor.author	Liao, Y https://orcid.org/0000-0002-8492-2648	en_US
dc.contributor.author	Ye, J https://orcid.org/0000-0002-6476-6406	en_US
dc.contributor.author	Kuchel, RP	en_US
dc.contributor.author	Poljak, A	en_US
dc.contributor.author	Raftery, MJ	en_US
dc.contributor.author	Cavicchioli, R	en_US
dc.date.available	2017-02-08	en_US
dc.date.issued	2017-06-01	en_US
dc.identifier.citation	Environmental Microbiology, 2017, 19 (6), pp. 2210 - 2227	en_US
dc.identifier.issn	1462-2912	en_US
dc.identifier.uri	http://hdl.handle.net/10453/115456
dc.description.abstract	© 2017 Society for Applied Microbiology and John Wiley & Sons Ltd Halohasta litchfieldiae represents ∼ 44% and Halorubrum lacusprofundi ∼ 10% of the hypersaline, perennially cold (≥ −20°C) Deep Lake community in Antarctica. We used proteomics and microscopy to define physiological responses of these haloarchaea to growth at high (30°C) and low (10 and 4°C) temperatures. The proteomic data indicate that both species responded to low temperature by modifying their cell envelope including protein N-glycosylation, maintaining osmotic balance and translation initiation, and modifying RNA turnover and tRNA modification. Distinctions between the two species included DNA protection and repair strategies (e.g. roles of UspA and Rad50), and metabolism of glycerol and pyruvate. For Hrr. lacusprofundi, low temperature led to the formation of polyhydroxyalkanoate-like granules, with granule formation occurring by an unknown mechanism. Hrr. lacusprofundi also formed biofilms and synthesized high levels of Hsp20 chaperones. Hht. litchfieldiae was characterized by an active CRISPR system, and elevated levels of the core gene expression machinery, which contrasted markedly to the decreased levels of Hrr. lacusprofundi. These findings greatly expand the understanding of cellular mechanisms of cold adaptation in psychrophilic archaea, and provide insight into how Hht. litchfieldiae gains dominance in Deep Lake.	en_US
dc.relation.ispartof	Environmental Microbiology	en_US
dc.relation.isbasedon	10.1111/1462-2920.13705	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Cell Membrane	en_US
dc.subject.mesh	Biofilms	en_US
dc.subject.mesh	Membrane Proteins	en_US
dc.subject.mesh	RNA	en_US
dc.subject.mesh	Proteomics	en_US
dc.subject.mesh	Adaptation, Physiological	en_US
dc.subject.mesh	DNA Repair	en_US
dc.subject.mesh	Glycosylation	en_US
dc.subject.mesh	Antarctic Regions	en_US
dc.subject.mesh	HSP20 Heat-Shock Proteins	en_US
dc.subject.mesh	Polyhydroxyalkanoates	en_US
dc.subject.mesh	Cold Temperature	en_US
dc.subject.mesh	Halorubrum	en_US
dc.subject.mesh	Lakes	en_US
dc.title	Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	19	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0603 Evolutionary Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	19	en_US

Abstract:

© 2017 Society for Applied Microbiology and John Wiley & Sons Ltd Halohasta litchfieldiae represents ∼ 44% and Halorubrum lacusprofundi ∼ 10% of the hypersaline, perennially cold (≥ −20°C) Deep Lake community in Antarctica. We used proteomics and microscopy to define physiological responses of these haloarchaea to growth at high (30°C) and low (10 and 4°C) temperatures. The proteomic data indicate that both species responded to low temperature by modifying their cell envelope including protein N-glycosylation, maintaining osmotic balance and translation initiation, and modifying RNA turnover and tRNA modification. Distinctions between the two species included DNA protection and repair strategies (e.g. roles of UspA and Rad50), and metabolism of glycerol and pyruvate. For Hrr. lacusprofundi, low temperature led to the formation of polyhydroxyalkanoate-like granules, with granule formation occurring by an unknown mechanism. Hrr. lacusprofundi also formed biofilms and synthesized high levels of Hsp20 chaperones. Hht. litchfieldiae was characterized by an active CRISPR system, and elevated levels of the core gene expression machinery, which contrasted markedly to the decreased levels of Hrr. lacusprofundi. These findings greatly expand the understanding of cellular mechanisms of cold adaptation in psychrophilic archaea, and provide insight into how Hht. litchfieldiae gains dominance in Deep Lake.

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http://hdl.handle.net/10453/115456