The presence and role of bacterial quorum sensing in activated sludge

Chong, G; Kimyon, O; Rice, SA; Kjelleberg, S; Manefield, M

The presence and role of bacterial quorum sensing in activated sludge

Chong, G Kimyon, O Rice, SA

Kjelleberg, S Manefield, M

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Publication Type:: Journal Article
Citation:: Microbial Biotechnology, 2012, 5 (5), pp. 621 - 633
Issue Date:: 2012-09-01

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Field	Value	Language
dc.contributor.author	Chong, G	en_US
dc.contributor.author	Kimyon, O	en_US
dc.contributor.author	Rice, SA https://orcid.org/0000-0002-9486-2343	en_US
dc.contributor.author	Kjelleberg, S	en_US
dc.contributor.author	Manefield, M	en_US
dc.date.available	2012-04-05	en_US
dc.date.issued	2012-09-01	en_US
dc.identifier.citation	Microbial Biotechnology, 2012, 5 (5), pp. 621 - 633	en_US
dc.identifier.issn	1751-7907	en_US
dc.identifier.uri	http://hdl.handle.net/10453/115464
dc.description.abstract	Activated sludge used for wastewater treatment globally is composed of a high-density microbial community of great biotechnological significance. In this study the presence and purpose of quorum sensing via N-acylated-l-homoserine lactones (AHLs) in activated sludge was explored. The presence of N-heptanoyl-l-homoserine lactone in organic extracts of sludge was demonstrated along with activation of a LuxR-based AHL monitor strain deployed in sludge, indicating AHL-mediated gene expression is active in sludge flocculates but not in the bulk aqueous phase. Bacterial isolates from activated sludge were screened for AHL production and expression of phenotypes commonly but not exclusively regulated by AHL-mediated gene transcription. N-acylated-l-homoserine lactone and exoenzyme production were frequently observed among the isolates. N-acylated-l-homoserine lactone addition to sludge upregulated chitinase activity and an AHL- and chitinase-producing isolate closely related to Aeromonas hydrophila was shown to respond to AHL addition with upregulation of chitinase activity. N-acylated-l-homoserine lactones produced by this strain were identified and genes ahyI/R and chiA, encoding AHL production and response and chitinase activity respectively, were sequenced. These experiments provide insight into the relationship between AHL-mediated gene expression and exoenzyme activity in activated sludge and may ultimately create opportunities to improve sludge performance. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.	en_US
dc.relation.ispartof	Microbial Biotechnology	en_US
dc.relation.isbasedon	10.1111/j.1751-7915.2012.00348.x	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Sewage	en_US
dc.subject.mesh	Genes, Bacterial	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Quorum Sensing	en_US
dc.subject.mesh	Acyl-Butyrolactones	en_US
dc.subject.mesh	Bacterial Physiological Phenomena	en_US
dc.subject.mesh	Metagenome	en_US
dc.title	The presence and role of bacterial quorum sensing in activated sludge	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	5	en_US

Abstract:

Activated sludge used for wastewater treatment globally is composed of a high-density microbial community of great biotechnological significance. In this study the presence and purpose of quorum sensing via N-acylated-l-homoserine lactones (AHLs) in activated sludge was explored. The presence of N-heptanoyl-l-homoserine lactone in organic extracts of sludge was demonstrated along with activation of a LuxR-based AHL monitor strain deployed in sludge, indicating AHL-mediated gene expression is active in sludge flocculates but not in the bulk aqueous phase. Bacterial isolates from activated sludge were screened for AHL production and expression of phenotypes commonly but not exclusively regulated by AHL-mediated gene transcription. N-acylated-l-homoserine lactone and exoenzyme production were frequently observed among the isolates. N-acylated-l-homoserine lactone addition to sludge upregulated chitinase activity and an AHL- and chitinase-producing isolate closely related to Aeromonas hydrophila was shown to respond to AHL addition with upregulation of chitinase activity. N-acylated-l-homoserine lactones produced by this strain were identified and genes ahyI/R and chiA, encoding AHL production and response and chitinase activity respectively, were sequenced. These experiments provide insight into the relationship between AHL-mediated gene expression and exoenzyme activity in activated sludge and may ultimately create opportunities to improve sludge performance. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/115464