Strigolactone analog GR24 triggers changes in PIN2 polarity, vesicle trafficking and actin filament architecture

Publisher:
Wiley
Publication Type:
Journal Article
Citation:
New Phytologist, 2014, 202 (4), pp. 1184 - 1196 (13)
Issue Date:
2014-06-01
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SummaryStrigolactones (SLs) are plant hormones that regulate shoot and root development in aMAX2-dependent manner. The mechanism underlying SLs’ effects on roots is unclear.We used root hair elongation to measure root response to SLs. We examined the effects ofGR24 (a synthetic, biologically active SL analog) on localization of the auxin efflux transporterPIN2, endosomal trafficking, and F-actin architecture and dynamics in the plasma membrane(PM) of epidermal cells of the primary root elongation zone in wildtype (WT) Arabidopsis andthe SL-insensitive mutant max2. We also recorded the response to GR24 of trafficking (tir3),actin (der1) and PIN2 (eir1) mutants.GR24 increased polar localization of PIN2 in the PM of epidermal cells and accumulation ofPIN2-containing brefeldin A (BFA) bodies, increased ARA7-labeled endosomal trafficking,reduced F-actin bundling and enhanced actin dynamics, all in a MAX2-dependent manner.Most of the der1 and tir3 mutant lines also displayed reduced sensitivity to GR24 with respectto root hair elongation.We suggest that SLs increase PIN2 polar localization, PIN2 endocytosis, endosomal traffick-ing, actin debundling and actin dynamics in a MAX2-dependent fashion. This enhancementmight underlie the WT root’s response to SLs, and suggests noncell autonomous activity ofSLs in roots
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