In Nicotiana species, an artificial microRNA corresponding to the virulence modulating region of Potato spindle tuber viroid directs RNA silencing of a soluble inorganic pyrophosphatase gene and the development of abnormal phenotypes

Eamens, AL; Smith, NA; Dennis, ES; Wassenegger, M; Wang, MB

In Nicotiana species, an artificial microRNA corresponding to the virulence modulating region of Potato spindle tuber viroid directs RNA silencing of a soluble inorganic pyrophosphatase gene and the development of abnormal phenotypes

Eamens, AL Smith, NA Dennis, ES

Wassenegger, M Wang, MB

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Publication Type:: Journal Article
Citation:: Virology, 2014, 450-451 pp. 266 - 277
Issue Date:: 2014-02-01

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Field	Value	Language
dc.contributor.author	Eamens, AL	en_US
dc.contributor.author	Smith, NA	en_US
dc.contributor.author	Dennis, ES https://orcid.org/0000-0002-7850-591X	en_US
dc.contributor.author	Wassenegger, M	en_US
dc.contributor.author	Wang, MB	en_US
dc.date.available	2013-12-15	en_US
dc.date.issued	2014-02-01	en_US
dc.identifier.citation	Virology, 2014, 450-451 pp. 266 - 277	en_US
dc.identifier.issn	0042-6822	en_US
dc.identifier.uri	http://hdl.handle.net/10453/115923
dc.description.abstract	Potato spindle tuber viroid (PSTVd) is a small non-protein-coding RNA pathogen that can induce disease symptoms in a variety of plant species. How PSTVd induces disease symptoms is a long standing question. It has been suggested that PSTVd-derived small RNAs (sRNAs) could direct RNA silencing of a targeted host gene(s) resulting in symptom development. To test this, we expressed PSTVd sequences as artificial microRNAs (amiRNAs) in Nicotiana tabacum and Nicotiana benthamiana. One amiRNA, amiR46 that corresponds to sequences within the PSTVd virulence modulating region (VMR), induced abnormal phenotypes in both Nicotiana species that closely resemble those displayed by PSTVd infected plants. In N. tabacum amiR46 plants, phenotype severity correlated with amiR46 accumulation and expression down-regulation of the bioinformatically-identified target gene, a Nicotiana soluble inorganic pyrophosphatase (siPPase). Taken together, our phenotypic and molecular analyses suggest that disease symptom development in Nicotiana species following PSTVd infection results from sRNA-directed RNA silencing of the host gene, siPPase. © 2013 Elsevier Inc.	en_US
dc.relation.ispartof	Virology	en_US
dc.relation.isbasedon	10.1016/j.virol.2013.12.019	en_US
dc.subject.classification	Virology	en_US
dc.subject.mesh	Viroids	en_US
dc.subject.mesh	Tobacco	en_US
dc.subject.mesh	Inorganic Pyrophosphatase	en_US
dc.subject.mesh	Plant Proteins	en_US
dc.subject.mesh	MicroRNAs	en_US
dc.subject.mesh	RNA, Viral	en_US
dc.subject.mesh	Virulence	en_US
dc.subject.mesh	Plant Diseases	en_US
dc.subject.mesh	Gene Silencing	en_US
dc.subject.mesh	Base Sequence	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.title	In Nicotiana species, an artificial microRNA corresponding to the virulence modulating region of Potato spindle tuber viroid directs RNA silencing of a soluble inorganic pyrophosphatase gene and the development of abnormal phenotypes	en_US
dc.type	Journal Article
utslib.citation.volume	450-451	en_US
utslib.for	0604 Genetics	en_US
utslib.for	060705 Plant Physiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	450-451	en_US

Abstract:

Potato spindle tuber viroid (PSTVd) is a small non-protein-coding RNA pathogen that can induce disease symptoms in a variety of plant species. How PSTVd induces disease symptoms is a long standing question. It has been suggested that PSTVd-derived small RNAs (sRNAs) could direct RNA silencing of a targeted host gene(s) resulting in symptom development. To test this, we expressed PSTVd sequences as artificial microRNAs (amiRNAs) in Nicotiana tabacum and Nicotiana benthamiana. One amiRNA, amiR46 that corresponds to sequences within the PSTVd virulence modulating region (VMR), induced abnormal phenotypes in both Nicotiana species that closely resemble those displayed by PSTVd infected plants. In N. tabacum amiR46 plants, phenotype severity correlated with amiR46 accumulation and expression down-regulation of the bioinformatically-identified target gene, a Nicotiana soluble inorganic pyrophosphatase (siPPase). Taken together, our phenotypic and molecular analyses suggest that disease symptom development in Nicotiana species following PSTVd infection results from sRNA-directed RNA silencing of the host gene, siPPase. © 2013 Elsevier Inc.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/115923