Coordination of chromosome segregation and cell division in Staphylococcus aureus

Bottomley, AL; Liew, ATF; Kusuma, KD; Peterson, E; Seidel, L; Foster, SJ; Harry, EJ

Coordination of chromosome segregation and cell division in Staphylococcus aureus

Bottomley, AL

Liew, ATF Kusuma, KD Peterson, E Seidel, L Foster, SJ Harry, EJ

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Publication Type:: Journal Article
Citation:: Frontiers in Microbiology, 2017, 8 (AUG)
Issue Date:: 2017-08-23

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Field	Value	Language
dc.contributor.author	Bottomley, AL https://orcid.org/0000-0003-0255-0869	en_US
dc.contributor.author	Liew, ATF	en_US
dc.contributor.author	Kusuma, KD	en_US
dc.contributor.author	Peterson, E	en_US
dc.contributor.author	Seidel, L	en_US
dc.contributor.author	Foster, SJ	en_US
dc.contributor.author	Harry, EJ https://orcid.org/0000-0003-0858-9473	en_US
dc.date.available	2017-08-23	en_US
dc.date.issued	2017-08-23	en_US
dc.identifier.citation	Frontiers in Microbiology, 2017, 8 (AUG)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/116151
dc.description.abstract	© 2017 Bottomley, Liew, Kusuma, Peterson, Seidel, Foster and Harry. Productive bacterial cell division and survival of progeny requires tight coordination between chromosome segregation and cell division to ensure equal partitioning of DNA. Unlike rod-shaped bacteria that undergo division in one plane, the coccoid human pathogen Staphylococcus aureus divides in three successive orthogonal planes, which requires a different spatial control compared to rod-shaped cells. To gain a better understanding of how this coordination between chromosome segregation and cell division is regulated in S. aureus, we investigated proteins that associate with FtsZ and the divisome. We found that DnaK, a well-known chaperone, interacts with FtsZ, EzrA and DivIVA, and is required for DivIVA stability. Unlike in several rod shaped organisms, DivIVA in S. aureus associates with several components of the divisome, as well as the chromosome segregation protein, SMC. This data, combined with phenotypic analysis of mutants, suggests a novel role for S. aureus DivIVA in ensuring cell division and chromosome segregation are coordinated.	en_US
dc.relation	http://purl.org/au-research/grants/arc/RN0460002
dc.relation	http://purl.org/au-research/grants/nhmrc/APP1021479
dc.relation.ispartof	Frontiers in Microbiology	en_US
dc.relation.isbasedon	10.3389/fmicb.2017.01575	en_US
dc.title	Coordination of chromosome segregation and cell division in Staphylococcus aureus	en_US
dc.type	Journal Article
utslib.citation.volume	AUG	en_US
utslib.citation.volume	8	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0502 Environmental Science and Management	en_US
utslib.for	0503 Soil Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	AUG	en_US
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

© 2017 Bottomley, Liew, Kusuma, Peterson, Seidel, Foster and Harry. Productive bacterial cell division and survival of progeny requires tight coordination between chromosome segregation and cell division to ensure equal partitioning of DNA. Unlike rod-shaped bacteria that undergo division in one plane, the coccoid human pathogen Staphylococcus aureus divides in three successive orthogonal planes, which requires a different spatial control compared to rod-shaped cells. To gain a better understanding of how this coordination between chromosome segregation and cell division is regulated in S. aureus, we investigated proteins that associate with FtsZ and the divisome. We found that DnaK, a well-known chaperone, interacts with FtsZ, EzrA and DivIVA, and is required for DivIVA stability. Unlike in several rod shaped organisms, DivIVA in S. aureus associates with several components of the divisome, as well as the chromosome segregation protein, SMC. This data, combined with phenotypic analysis of mutants, suggests a novel role for S. aureus DivIVA in ensuring cell division and chromosome segregation are coordinated.

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http://hdl.handle.net/10453/116151