Immobilization techniques of bacteria for live super-resolution imaging using structured illumination microscopy

Bottomley, AL; Turnbull, L; Whitchurch, CB; Harry, EJ

Immobilization techniques of bacteria for live super-resolution imaging using structured illumination microscopy

Bottomley, AL

Turnbull, L

Whitchurch, CB

Harry, EJ

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Publication Type:: Chapter
Citation:: Methods in Molecular Biology, 2017, 1535 pp. 197 - 209
Issue Date:: 2017-01-01

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Field	Value	Language
dc.contributor.author	Bottomley, AL https://orcid.org/0000-0003-0255-0869	en_US
dc.contributor.author	Turnbull, L https://orcid.org/0000-0002-9255-9033	en_US
dc.contributor.author	Whitchurch, CB https://orcid.org/0000-0003-2296-3791	en_US
dc.contributor.author	Harry, EJ https://orcid.org/0000-0003-0858-9473	en_US
dc.date.issued	2017-01-01	en_US
dc.identifier.citation	Methods in Molecular Biology, 2017, 1535 pp. 197 - 209	en_US
dc.identifier.uri	http://hdl.handle.net/10453/116152
dc.description.abstract	© Springer Science+Business Media New York 2017. Advancements in optical microscopy technology have allowed huge progression in the ability to understand protein structure and dynamics in live bacterial cells using fluorescence microscopy. Paramount to high quality microscopy is good sample preparation to avoid bacterial cell movement that can result in motion blur during image acquisition. Here, we describe two techniques of sample preparation that reduce unwanted cell movement and are suitable for application to a number of bacterial species and imaging methods.	en_US
dc.relation.ispartof	Methods in Molecular Biology	en_US
dc.relation.isbasedon	10.1007/978-1-4939-6673-8_12	en_US
dc.subject.classification	Developmental Biology	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	Imaging, Three-Dimensional	en_US
dc.subject.mesh	Microscopy, Fluorescence	en_US
dc.subject.mesh	Image Processing, Computer-Assisted	en_US
dc.title	Immobilization techniques of bacteria for live super-resolution imaging using structured illumination microscopy	en_US
dc.type	Chapter
utslib.citation.volume	1535	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	0399 Other Chemical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	1535	en_US

Abstract:

© Springer Science+Business Media New York 2017. Advancements in optical microscopy technology have allowed huge progression in the ability to understand protein structure and dynamics in live bacterial cells using fluorescence microscopy. Paramount to high quality microscopy is good sample preparation to avoid bacterial cell movement that can result in motion blur during image acquisition. Here, we describe two techniques of sample preparation that reduce unwanted cell movement and are suitable for application to a number of bacterial species and imaging methods.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/116152