A microfluidics-based in situ chemotaxis assay to study the behaviour of aquatic microbial communities

Lambert, BS; Raina, JB; Fernandez, VI; Rinke, C; Siboni, N; Rubino, F; Hugenholtz, P; Tyson, GW; Seymour, JR; Stocker, R

A microfluidics-based in situ chemotaxis assay to study the behaviour of aquatic microbial communities

Lambert, BS

Raina, JB

Fernandez, VI Rinke, C Siboni, N

Rubino, F Hugenholtz, P Tyson, GW Seymour, JR

Stocker, R

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Publication Type:: Journal Article
Citation:: Nature Microbiology, 2017, 2 (10), pp. 1344 - 1349
Issue Date:: 2017-10-01

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Field	Value	Language
dc.contributor.author	Lambert, BS https://orcid.org/0000-0002-4831-3255	en_US
dc.contributor.author	Raina, JB https://orcid.org/0000-0002-7508-0004	en_US
dc.contributor.author	Fernandez, VI	en_US
dc.contributor.author	Rinke, C	en_US
dc.contributor.author	Siboni, N https://orcid.org/0000-0001-6082-0949	en_US
dc.contributor.author	Rubino, F	en_US
dc.contributor.author	Hugenholtz, P	en_US
dc.contributor.author	Tyson, GW	en_US
dc.contributor.author	Seymour, JR https://orcid.org/0000-0002-3745-6541	en_US
dc.contributor.author	Stocker, R	en_US
dc.date.available	2020-05-25T19:18:32Z
dc.date.issued	2017-10-01	en_US
dc.identifier.citation	Nature Microbiology, 2017, 2 (10), pp. 1344 - 1349	en_US
dc.identifier.uri	http://hdl.handle.net/10453/116326
dc.description.abstract	© 2017 The Author(s). Microbial interactions influence the productivity and biogeochemistry of the ocean, yet they occur in miniscule volumes that cannot be sampled by traditional oceanographic techniques. To investigate the behaviours of marine microorganisms at spatially relevant scales, we engineered an in situ chemotaxis assay (ISCA) based on microfluidic technology. Here, we describe the fabrication, testing and first field results of the ISCA, demonstrating its value in accessing the microbial behaviours that shape marine ecosystems.	en_US
dc.relation	http://purl.org/au-research/grants/arc/DP110103091
dc.relation	http://purl.org/au-research/grants/arc/DE160100636
dc.relation.ispartof	Nature Microbiology	en_US
dc.relation.isbasedon	10.1038/s41564-017-0010-9	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	RNA, Ribosomal, 16S	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Microfluidics	en_US
dc.subject.mesh	Water Microbiology	en_US
dc.subject.mesh	Ecosystem	en_US
dc.subject.mesh	Seawater	en_US
dc.subject.mesh	Chemotaxis	en_US
dc.subject.mesh	Models, Theoretical	en_US
dc.subject.mesh	Models, Biological	en_US
dc.subject.mesh	Oceans and Seas	en_US
dc.subject.mesh	Bacterial Physiological Phenomena	en_US
dc.subject.mesh	Microbial Interactions	en_US
dc.subject.mesh	Aquatic Organisms	en_US
dc.title	A microfluidics-based in situ chemotaxis assay to study the behaviour of aquatic microbial communities	en_US
dc.type	Journal Article
utslib.citation.volume	10	en_US
utslib.citation.volume	2	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	1108 Medical Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	open_access
pubs.issue	10	en_US
pubs.publication-status	Published	en_US
pubs.volume	2	en_US

Abstract:

© 2017 The Author(s). Microbial interactions influence the productivity and biogeochemistry of the ocean, yet they occur in miniscule volumes that cannot be sampled by traditional oceanographic techniques. To investigate the behaviours of marine microorganisms at spatially relevant scales, we engineered an in situ chemotaxis assay (ISCA) based on microfluidic technology. Here, we describe the fabrication, testing and first field results of the ISCA, demonstrating its value in accessing the microbial behaviours that shape marine ecosystems.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/116326