Genetic and physical mapping of flowering time loci in canola (Brassica napus L.)

Raman, H; Raman, R; Eckermann, P; Coombes, N; Manoli, S; Zou, X; Edwards, D; Meng, J; Prangnell, R; Stiller, J; Batley, J; Luckett, D; Wratten, N; Dennis, E

Genetic and physical mapping of flowering time loci in canola (Brassica napus L.)

Raman, H Raman, R Eckermann, P Coombes, N Manoli, S Zou, X Edwards, D Meng, J Prangnell, R Stiller, J Batley, J Luckett, D Wratten, N Dennis, E

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Publication Type:: Journal Article
Citation:: Theoretical and Applied Genetics, 2013, 126 (1), pp. 119 - 132
Issue Date:: 2013-01-01

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Field	Value	Language
dc.contributor.author	Raman, H	en_US
dc.contributor.author	Raman, R	en_US
dc.contributor.author	Eckermann, P	en_US
dc.contributor.author	Coombes, N	en_US
dc.contributor.author	Manoli, S	en_US
dc.contributor.author	Zou, X	en_US
dc.contributor.author	Edwards, D	en_US
dc.contributor.author	Meng, J	en_US
dc.contributor.author	Prangnell, R	en_US
dc.contributor.author	Stiller, J	en_US
dc.contributor.author	Batley, J	en_US
dc.contributor.author	Luckett, D	en_US
dc.contributor.author	Wratten, N	en_US
dc.contributor.author	Dennis, E https://orcid.org/0000-0002-7850-591X	en_US
dc.date.available	2012-08-10	en_US
dc.date.issued	2013-01-01	en_US
dc.identifier.citation	Theoretical and Applied Genetics, 2013, 126 (1), pp. 119 - 132	en_US
dc.identifier.issn	0040-5752	en_US
dc.identifier.uri	http://hdl.handle.net/10453/116452
dc.description.abstract	We identified quantitative trait loci (QTL) underlying variation for flowering time in a doubled haploid (DH) population of vernalisation-responsive canola (Brassica napus L.) cultivars Skipton and Ag-Spectrum and aligned them with physical map positions of predicted flowering genes from the Brassica rapa genome. Significant genetic variation in flowering time and response to vernalisation were observed among the DH lines from Skipton/Ag-Spectrum. A molecular linkage map was generated comprising 674 simple sequence repeat, sequence-related amplified polymorphism, sequence characterised amplified region, Diversity Array Technology, and candidate gene based markers loci. QTL analysis indicated that flowering time is a complex trait and is controlled by at least 20 loci, localised on ten different chromosomes. These loci each accounted for between 2. 4 and 28. 6 % of the total genotypic variation for first flowering and response to vernalisation. However, identification of consistent QTL was found to be dependant upon growing environments. We compared the locations of QTL with the physical positions of predicted flowering time genes located on the sequenced genome of B. rapa. Some QTL associated with flowering time on A02, A03, A07, and C06 may represent homologues of known flowering time genes in Arabidopsis; VERNALISATION INSENSITIVE 3, APETALA1, CAULIFLOWER, FLOWERING LOCUS C, FLOWERING LOCUS T, CURLY LEAF, SHORT VEGETATIVE PHASE, GA3 OXIDASE, and LEAFY. Identification of the chromosomal location and effect of the genes influencing flowering time may hasten the development of canola varieties having an optimal time for flowering in target environments such as for low rainfall areas, via marker-assisted selection. © 2012 Springer-Verlag.	en_US
dc.relation.ispartof	Theoretical and Applied Genetics	en_US
dc.relation.isbasedon	10.1007/s00122-012-1966-8	en_US
dc.subject.classification	Plant Biology & Botany	en_US
dc.subject.mesh	Brassica napus	en_US
dc.subject.mesh	Flowers	en_US
dc.subject.mesh	DNA, Plant	en_US
dc.subject.mesh	Genetic Markers	en_US
dc.subject.mesh	Models, Statistical	en_US
dc.subject.mesh	Chromosome Mapping	en_US
dc.subject.mesh	Physical Chromosome Mapping	en_US
dc.subject.mesh	Crosses, Genetic	en_US
dc.subject.mesh	Alleles	en_US
dc.subject.mesh	Genes, Plant	en_US
dc.subject.mesh	Genome, Plant	en_US
dc.subject.mesh	Quantitative Trait Loci	en_US
dc.subject.mesh	Models, Genetic	en_US
dc.subject.mesh	Genetic Variation	en_US
dc.subject.mesh	Genetic Linkage	en_US
dc.title	Genetic and physical mapping of flowering time loci in canola (Brassica napus L.)	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	126	en_US
utslib.for	0604 Genetics	en_US
utslib.for	060705 Plant Physiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	10 Technology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	126	en_US

Abstract:

We identified quantitative trait loci (QTL) underlying variation for flowering time in a doubled haploid (DH) population of vernalisation-responsive canola (Brassica napus L.) cultivars Skipton and Ag-Spectrum and aligned them with physical map positions of predicted flowering genes from the Brassica rapa genome. Significant genetic variation in flowering time and response to vernalisation were observed among the DH lines from Skipton/Ag-Spectrum. A molecular linkage map was generated comprising 674 simple sequence repeat, sequence-related amplified polymorphism, sequence characterised amplified region, Diversity Array Technology, and candidate gene based markers loci. QTL analysis indicated that flowering time is a complex trait and is controlled by at least 20 loci, localised on ten different chromosomes. These loci each accounted for between 2. 4 and 28. 6 % of the total genotypic variation for first flowering and response to vernalisation. However, identification of consistent QTL was found to be dependant upon growing environments. We compared the locations of QTL with the physical positions of predicted flowering time genes located on the sequenced genome of B. rapa. Some QTL associated with flowering time on A02, A03, A07, and C06 may represent homologues of known flowering time genes in Arabidopsis; VERNALISATION INSENSITIVE 3, APETALA1, CAULIFLOWER, FLOWERING LOCUS C, FLOWERING LOCUS T, CURLY LEAF, SHORT VEGETATIVE PHASE, GA3 OXIDASE, and LEAFY. Identification of the chromosomal location and effect of the genes influencing flowering time may hasten the development of canola varieties having an optimal time for flowering in target environments such as for low rainfall areas, via marker-assisted selection. © 2012 Springer-Verlag.

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http://hdl.handle.net/10453/116452