Chemosensory genes identified in the antennal transcriptome of the blowfly Calliphora stygia

Leitch, O; Papanicolaou, A; Lennard, C; Kirkbride, KP; Anderson, A

Chemosensory genes identified in the antennal transcriptome of the blowfly Calliphora stygia

Leitch, O

Papanicolaou, A Lennard, C

Kirkbride, KP

Anderson, A

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Publication Type:: Journal Article
Citation:: BMC Genomics, 2015, 16 (1)
Issue Date:: 2015-03-31

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Field	Value	Language
dc.contributor.author	Leitch, O https://orcid.org/0000-0003-4968-4653	en_US
dc.contributor.author	Papanicolaou, A	en_US
dc.contributor.author	Lennard, C https://orcid.org/0000-0002-9164-7103	en_US
dc.contributor.author	Kirkbride, KP https://orcid.org/0000-0001-7666-4039	en_US
dc.contributor.author	Anderson, A	en_US
dc.date.available	2015-03-16	en_US
dc.date.issued	2015-03-31	en_US
dc.identifier.citation	BMC Genomics, 2015, 16 (1)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/116456
dc.description.abstract	© 2015 Leitch et al. Background: Blowflies have relevance in areas of forensic science, agriculture, and medicine, primarily due to the ability of their larvae to develop on flesh. While it is widely accepted that blowflies rely heavily on olfaction for identifying and locating hosts, there is limited research regarding the underlying molecular mechanisms. Using next generation sequencing (Illumina), this research examined the antennal transcriptome of Calliphora stygia (Fabricius) (Diptera: Calliphoridae) to identify members of the major chemosensory gene families necessary for olfaction. Results: Representative proteins from all chemosensory gene families essential in insect olfaction were identified in the antennae of the blowfly C. stygia, including 50 odorant receptors, 22 ionotropic receptors, 21 gustatory receptors, 28 odorant binding proteins, 4 chemosensory proteins, and 3 sensory neuron membrane proteins. A total of 97 candidate cytochrome P450s and 39 esterases, some of which may act as odorant degrading enzymes, were also identified. Importantly, co-receptors necessary for the proper function of ligand-binding receptors were identified. Putative orthologues for the conserved antennal ionotropic receptors and candidate gustatory receptors for carbon dioxide detection were also amongst the identified proteins. Conclusions: This research provides a comprehensive novel resource that will be fundamental for future studies regarding blowfly olfaction. Such information presents potential benefits to the forensic, pest control, and medical areas, and could assist in the understanding of insecticide resistance and targeted control through cross-species comparisons.	en_US
dc.relation.ispartof	BMC Genomics	en_US
dc.relation.isbasedon	10.1186/s12864-015-1466-8	en_US
dc.subject.classification	Bioinformatics	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Diptera	en_US
dc.subject.mesh	Drosophila	en_US
dc.subject.mesh	Insect Proteins	en_US
dc.subject.mesh	Membrane Proteins	en_US
dc.subject.mesh	Receptors, Cell Surface	en_US
dc.subject.mesh	Receptors, Odorant	en_US
dc.subject.mesh	Sequence Alignment	en_US
dc.subject.mesh	Smell	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Amino Acid Sequence	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Genome, Insect	en_US
dc.subject.mesh	Arthropod Antennae	en_US
dc.subject.mesh	Transcriptome	en_US
dc.title	Chemosensory genes identified in the antennal transcriptome of the blowfly Calliphora stygia	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	16	en_US
utslib.for	060808 Invertebrate Biology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	08 Information and Computing Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	16	en_US

Abstract:

© 2015 Leitch et al. Background: Blowflies have relevance in areas of forensic science, agriculture, and medicine, primarily due to the ability of their larvae to develop on flesh. While it is widely accepted that blowflies rely heavily on olfaction for identifying and locating hosts, there is limited research regarding the underlying molecular mechanisms. Using next generation sequencing (Illumina), this research examined the antennal transcriptome of Calliphora stygia (Fabricius) (Diptera: Calliphoridae) to identify members of the major chemosensory gene families necessary for olfaction. Results: Representative proteins from all chemosensory gene families essential in insect olfaction were identified in the antennae of the blowfly C. stygia, including 50 odorant receptors, 22 ionotropic receptors, 21 gustatory receptors, 28 odorant binding proteins, 4 chemosensory proteins, and 3 sensory neuron membrane proteins. A total of 97 candidate cytochrome P450s and 39 esterases, some of which may act as odorant degrading enzymes, were also identified. Importantly, co-receptors necessary for the proper function of ligand-binding receptors were identified. Putative orthologues for the conserved antennal ionotropic receptors and candidate gustatory receptors for carbon dioxide detection were also amongst the identified proteins. Conclusions: This research provides a comprehensive novel resource that will be fundamental for future studies regarding blowfly olfaction. Such information presents potential benefits to the forensic, pest control, and medical areas, and could assist in the understanding of insecticide resistance and targeted control through cross-species comparisons.

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http://hdl.handle.net/10453/116456