Models to study airway smooth muscle contraction in vivo, ex vivo and in vitro: Implications in understanding asthma

Wright, D; Sharma, P; Ryu, MH; Rissé, PA; Ngo, M; Maarsingh, H; Koziol-White, C; Jha, A; Halayko, AJ; West, AR

Models to study airway smooth muscle contraction in vivo, ex vivo and in vitro: Implications in understanding asthma

Wright, D Sharma, P

Ryu, MH Rissé, PA Ngo, M Maarsingh, H Koziol-White, C Jha, A Halayko, AJ West, AR

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Publication Type:: Journal Article
Citation:: Pulmonary Pharmacology and Therapeutics, 2013, 26 (1), pp. 24 - 36
Issue Date:: 2013-02-01

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Field	Value	Language
dc.contributor.author	Wright, D	en_US
dc.contributor.author	Sharma, P https://orcid.org/0000-0002-2904-2306	en_US
dc.contributor.author	Ryu, MH	en_US
dc.contributor.author	Rissé, PA	en_US
dc.contributor.author	Ngo, M	en_US
dc.contributor.author	Maarsingh, H	en_US
dc.contributor.author	Koziol-White, C	en_US
dc.contributor.author	Jha, A	en_US
dc.contributor.author	Halayko, AJ	en_US
dc.contributor.author	West, AR	en_US
dc.date.available	2012-08-17	en_US
dc.date.issued	2013-02-01	en_US
dc.identifier.citation	Pulmonary Pharmacology and Therapeutics, 2013, 26 (1), pp. 24 - 36	en_US
dc.identifier.issn	1094-5539	en_US
dc.identifier.uri	http://hdl.handle.net/10453/116593
dc.description.abstract	Asthma is a chronic obstructive airway disease characterised by airway hyperresponsiveness (AHR) and airway wall remodelling. The effector of airway narrowing is the contraction of airway smooth muscle (ASM), yet the question of whether an inherent or acquired dysfunction in ASM contractile function plays a significant role in the disease pathophysiology remains contentious.The difficulty in determining the role of ASM lies in limitations with the models used to assess contraction. In vivo models provide a fully integrated physiological response but ASM contraction cannot be directly measured. Ex vivo and in vitro models can provide more direct assessment of ASM contraction but the loss of factors that may modulate ASM responsiveness and AHR, including interaction between multiple cell types and disruption of the mechanical environment, precludes a complete understanding of the disease process.In this review we detail key advantages of common in vivo, ex vivo and in vitro models of ASM contraction, as well as emerging tissue engineered models of ASM and whole airways. We also highlight important findings from each model with respect to the pathophysiology of asthma. © 2012 Elsevier Ltd.	en_US
dc.relation.ispartof	Pulmonary Pharmacology and Therapeutics	en_US
dc.relation.isbasedon	10.1016/j.pupt.2012.08.006	en_US
dc.subject.classification	Respiratory System	en_US
dc.subject.mesh	Muscle, Smooth	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Asthma	en_US
dc.subject.mesh	Bronchial Hyperreactivity	en_US
dc.subject.mesh	Disease Models, Animal	en_US
dc.subject.mesh	Tissue Engineering	en_US
dc.subject.mesh	Muscle Contraction	en_US
dc.subject.mesh	Models, Biological	en_US
dc.title	Models to study airway smooth muscle contraction in vivo, ex vivo and in vitro: Implications in understanding asthma	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	26	en_US
utslib.for	1103 Clinical Sciences	en_US
utslib.for	1115 Pharmacology and Pharmaceutical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	26	en_US

Abstract:

Asthma is a chronic obstructive airway disease characterised by airway hyperresponsiveness (AHR) and airway wall remodelling. The effector of airway narrowing is the contraction of airway smooth muscle (ASM), yet the question of whether an inherent or acquired dysfunction in ASM contractile function plays a significant role in the disease pathophysiology remains contentious.The difficulty in determining the role of ASM lies in limitations with the models used to assess contraction. In vivo models provide a fully integrated physiological response but ASM contraction cannot be directly measured. Ex vivo and in vitro models can provide more direct assessment of ASM contraction but the loss of factors that may modulate ASM responsiveness and AHR, including interaction between multiple cell types and disruption of the mechanical environment, precludes a complete understanding of the disease process.In this review we detail key advantages of common in vivo, ex vivo and in vitro models of ASM contraction, as well as emerging tissue engineered models of ASM and whole airways. We also highlight important findings from each model with respect to the pathophysiology of asthma. © 2012 Elsevier Ltd.

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http://hdl.handle.net/10453/116593