Influence of anode potentials on selection of Geobacter strains in microbial electrolysis cells

Commault, AS; Lear, G; Packer, MA; Weld, RJ

Influence of anode potentials on selection of Geobacter strains in microbial electrolysis cells

Commault, AS

Lear, G

Packer, MA Weld, RJ

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Publication Type:: Journal Article
Citation:: Bioresource Technology, 2013, 139 pp. 226 - 234
Issue Date:: 2013-01-01

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Field	Value	Language
dc.contributor.author	Commault, AS https://orcid.org/0000-0002-6730-0089	en_US
dc.contributor.author	Lear, G https://orcid.org/0000-0001-5083-4773	en_US
dc.contributor.author	Packer, MA	en_US
dc.contributor.author	Weld, RJ	en_US
dc.date.available	2013-04-12	en_US
dc.date.issued	2013-01-01	en_US
dc.identifier.citation	Bioresource Technology, 2013, 139 pp. 226 - 234	en_US
dc.identifier.issn	0960-8524	en_US
dc.identifier.uri	http://hdl.handle.net/10453/116660
dc.description.abstract	Through their ability to directly transfer electrons to electrodes, Geobacter sp. are key organisms for microbial fuel cell technology. This study presents a simple method to reproducibly select Geobacter-dominated anode biofilms from a mixed inoculum of bacteria using graphite electrodes initially poised at -0.25, -0.36 and -0.42V vs. Ag/AgCl. The biofilms all produced maximum power density of approximately 270mWm-2 (projected anode surface area). Analysis of 16S rRNA genes and intergenic spacer (ITS) sequences found that the biofilm communities were all dominated by bacteria closely related to Geobacter psychrophilus. Anodes initially poised at -0.25V reproducibly selected biofilms that were dominated by a strain of G. psychrophilus that was genetically distinct from the strain that dominated the -0.36 and -0.42V biofilms. This work demonstrates for the first time that closely related strains of Geobacter can have very different competitive advantages at different anode potentials. © 2013 Elsevier Ltd.	en_US
dc.relation.ispartof	Bioresource Technology	en_US
dc.relation.isbasedon	10.1016/j.biortech.2013.04.047	en_US
dc.subject.classification	Biotechnology	en_US
dc.subject.mesh	Biofilms	en_US
dc.subject.mesh	Geobacter	en_US
dc.subject.mesh	Electrophoresis, Agar Gel	en_US
dc.subject.mesh	Electrodes	en_US
dc.subject.mesh	Bioelectric Energy Sources	en_US
dc.subject.mesh	Electrolysis	en_US
dc.subject.mesh	Time Factors	en_US
dc.subject.mesh	Electrochemical Techniques	en_US
dc.title	Influence of anode potentials on selection of Geobacter strains in microbial electrolysis cells	en_US
dc.type	Journal Article
utslib.citation.volume	139	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	139	en_US

Abstract:

Through their ability to directly transfer electrons to electrodes, Geobacter sp. are key organisms for microbial fuel cell technology. This study presents a simple method to reproducibly select Geobacter-dominated anode biofilms from a mixed inoculum of bacteria using graphite electrodes initially poised at -0.25, -0.36 and -0.42V vs. Ag/AgCl. The biofilms all produced maximum power density of approximately 270mWm-2 (projected anode surface area). Analysis of 16S rRNA genes and intergenic spacer (ITS) sequences found that the biofilm communities were all dominated by bacteria closely related to Geobacter psychrophilus. Anodes initially poised at -0.25V reproducibly selected biofilms that were dominated by a strain of G. psychrophilus that was genetically distinct from the strain that dominated the -0.36 and -0.42V biofilms. This work demonstrates for the first time that closely related strains of Geobacter can have very different competitive advantages at different anode potentials. © 2013 Elsevier Ltd.

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http://hdl.handle.net/10453/116660