The Deinococcus radiodurans DR1245 Protein, a DdrB Partner Homologous to YbjN Proteins and Reminiscent of Type III Secretion System Chaperones

Norais, C; Servant, P; Bouthier-de-la-Tour, C; Coureux, PD; Ithurbide, S; Vannier, F; Guerin, PP; Dulberger, CL; Satyshur, KA; Keck, JL; Armengaud, J; Cox, MM; Sommer, S

The Deinococcus radiodurans DR1245 Protein, a DdrB Partner Homologous to YbjN Proteins and Reminiscent of Type III Secretion System Chaperones

Norais, C Servant, P Bouthier-de-la-Tour, C Coureux, PD Ithurbide, S

Vannier, F Guerin, PP Dulberger, CL Satyshur, KA Keck, JL Armengaud, J Cox, MM Sommer, S

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Publication Type:: Journal Article
Citation:: PLoS ONE, 2013, 8 (2)
Issue Date:: 2013-02-18

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Field	Value	Language
dc.contributor.author	Norais, C	en_US
dc.contributor.author	Servant, P	en_US
dc.contributor.author	Bouthier-de-la-Tour, C	en_US
dc.contributor.author	Coureux, PD	en_US
dc.contributor.author	Ithurbide, S https://orcid.org/0000-0002-1533-8472	en_US
dc.contributor.author	Vannier, F	en_US
dc.contributor.author	Guerin, PP	en_US
dc.contributor.author	Dulberger, CL	en_US
dc.contributor.author	Satyshur, KA	en_US
dc.contributor.author	Keck, JL	en_US
dc.contributor.author	Armengaud, J	en_US
dc.contributor.author	Cox, MM	en_US
dc.contributor.author	Sommer, S	en_US
dc.date.available	2013-01-04	en_US
dc.date.issued	2013-02-18	en_US
dc.identifier.citation	PLoS ONE, 2013, 8 (2)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/117112
dc.description.abstract	The bacterium Deinococcus radiodurans exhibits an extreme resistance to ionizing radiation. A small subset of Deinococcus genus-specific genes were shown to be up-regulated upon exposure to ionizing radiation and to play a role in genome reconstitution. These genes include an SSB-like protein called DdrB. Here, we identified a novel protein encoded by the dr1245 gene as an interacting partner of DdrB. A strain devoid of the DR1245 protein is impaired in growth, exhibiting a generation time approximately threefold that of the wild type strain while radioresistance is not affected. We determined the three-dimensional structure of DR1245, revealing a relationship with type III secretion system chaperones and YbjN family proteins. Thus, DR1245 may display some chaperone activity towards DdrB and possibly other substrates. © 2013 Norais et al.	en_US
dc.relation.ispartof	PLoS ONE	en_US
dc.relation.isbasedon	10.1371/journal.pone.0056558	en_US
dc.subject.classification	General Science & Technology	en_US
dc.subject.mesh	Deinococcus	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Molecular Chaperones	en_US
dc.subject.mesh	Sequence Alignment	en_US
dc.subject.mesh	Amino Acid Sequence	en_US
dc.subject.mesh	Base Sequence	en_US
dc.subject.mesh	Protein Conformation	en_US
dc.subject.mesh	Protein Binding	en_US
dc.subject.mesh	Mutation	en_US
dc.subject.mesh	Radiation Tolerance	en_US
dc.subject.mesh	Models, Molecular	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Bacterial Secretion Systems	en_US
dc.title	The Deinococcus radiodurans DR1245 Protein, a DdrB Partner Homologous to YbjN Proteins and Reminiscent of Type III Secretion System Chaperones	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	8	en_US
utslib.for	060501 Bacteriology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

The bacterium Deinococcus radiodurans exhibits an extreme resistance to ionizing radiation. A small subset of Deinococcus genus-specific genes were shown to be up-regulated upon exposure to ionizing radiation and to play a role in genome reconstitution. These genes include an SSB-like protein called DdrB. Here, we identified a novel protein encoded by the dr1245 gene as an interacting partner of DdrB. A strain devoid of the DR1245 protein is impaired in growth, exhibiting a generation time approximately threefold that of the wild type strain while radioresistance is not affected. We determined the three-dimensional structure of DR1245, revealing a relationship with type III secretion system chaperones and YbjN family proteins. Thus, DR1245 may display some chaperone activity towards DdrB and possibly other substrates. © 2013 Norais et al.

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http://hdl.handle.net/10453/117112