Discovery of cyanophage genomes which contain mitochondrial DNA polymerase

Chan, YW; Mohr, R; Millard, AD; Holmes, AB; Larkum, AW; Whitworth, AL; Mann, NH; Scanlan, DJ; Hess, WR; Clokie, MRJ

Discovery of cyanophage genomes which contain mitochondrial DNA polymerase

Chan, YW Mohr, R Millard, AD Holmes, AB Larkum, AW

Whitworth, AL Mann, NH Scanlan, DJ Hess, WR Clokie, MRJ

Permalink

Publication Type:: Journal Article
Citation:: Molecular Biology and Evolution, 2011, 28 (8), pp. 2269 - 2274
Issue Date:: 2011-08-01

Closed Access

	Filename	Description	Size
	watermark(1).pdf	Published Version	245.62 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Chan, YW	en_US
dc.contributor.author	Mohr, R	en_US
dc.contributor.author	Millard, AD	en_US
dc.contributor.author	Holmes, AB	en_US
dc.contributor.author	Larkum, AW https://orcid.org/0000-0002-0420-134X	en_US
dc.contributor.author	Whitworth, AL	en_US
dc.contributor.author	Mann, NH	en_US
dc.contributor.author	Scanlan, DJ	en_US
dc.contributor.author	Hess, WR	en_US
dc.contributor.author	Clokie, MRJ	en_US
dc.date.issued	2011-08-01	en_US
dc.identifier.citation	Molecular Biology and Evolution, 2011, 28 (8), pp. 2269 - 2274	en_US
dc.identifier.issn	0737-4038	en_US
dc.identifier.uri	http://hdl.handle.net/10453/117562
dc.description.abstract	DNA polymerase γ is a family A DNA polymerase responsible for the replication of mitochondrial DNA in eukaryotes. The origins of DNA polymerase γ have remained elusive because it is not present in any known bacterium, though it has been hypothesized that mitochondria may have inherited the enzyme by phage-mediated nonorthologous displacement. Here, we present an analysis of two full-length homologues of this gene, which were found in the genomes of two bacteriophages, which infect the chlorophyll-d containing cyanobacterium Acaryochloris marina. Phylogenetic analyses of these phage DNA polymerase γ proteins show that they branch deeply within the DNA polymerase γ clade and therefore share a common origin with their eukaryotic homologues. We also found homologues of these phage polymerases in the environmental Community Cyberinfrastructure for Advanced Microbial Ecology Research and Analysis (CAMERA) database, which fell in the same clade. An analysis of the CAMERA assemblies containing the environmental homologues together with the filter fraction metadata indicated some of these assemblies may be of bacterial origin. We also show that the phage-encoded DNA polymerase γ is highly transcribed as the phage genomes are replicated. These findings provide data that may assist in reconstructing the evolution of mitochondria. © 2011 The Author Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved.	en_US
dc.relation.ispartof	Molecular Biology and Evolution	en_US
dc.relation.isbasedon	10.1093/molbev/msr041	en_US
dc.subject.classification	Evolutionary Biology	en_US
dc.subject.mesh	Cyanobacteria	en_US
dc.subject.mesh	Bacteriophages	en_US
dc.subject.mesh	DNA-Directed DNA Polymerase	en_US
dc.subject.mesh	DNA, Mitochondrial	en_US
dc.subject.mesh	Evolution, Molecular	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Gene Expression Regulation, Viral	en_US
dc.subject.mesh	Genome, Viral	en_US
dc.title	Discovery of cyanophage genomes which contain mitochondrial DNA polymerase	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
utslib.citation.volume	28	en_US
utslib.for	0604 Genetics	en_US
utslib.for	0603 Evolutionary Biology	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.issue	8	en_US
pubs.publication-status	Published	en_US
pubs.volume	28	en_US

Abstract:

DNA polymerase γ is a family A DNA polymerase responsible for the replication of mitochondrial DNA in eukaryotes. The origins of DNA polymerase γ have remained elusive because it is not present in any known bacterium, though it has been hypothesized that mitochondria may have inherited the enzyme by phage-mediated nonorthologous displacement. Here, we present an analysis of two full-length homologues of this gene, which were found in the genomes of two bacteriophages, which infect the chlorophyll-d containing cyanobacterium Acaryochloris marina. Phylogenetic analyses of these phage DNA polymerase γ proteins show that they branch deeply within the DNA polymerase γ clade and therefore share a common origin with their eukaryotic homologues. We also found homologues of these phage polymerases in the environmental Community Cyberinfrastructure for Advanced Microbial Ecology Research and Analysis (CAMERA) database, which fell in the same clade. An analysis of the CAMERA assemblies containing the environmental homologues together with the filter fraction metadata indicated some of these assemblies may be of bacterial origin. We also show that the phage-encoded DNA polymerase γ is highly transcribed as the phage genomes are replicated. These findings provide data that may assist in reconstructing the evolution of mitochondria. © 2011 The Author Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/117562