Graphene surface-anchored fluorescence sensor for sensitive detection of MicroRNA coupled with enzyme-free signal amplification of hybridization chain reaction

Publication Type:
Journal Article
ACS Applied Materials and Interfaces, 2012, 4 (12), pp. 6450 - 6453
Issue Date:
Filename Description Size
am302268t.pdfPublished Version308.58 kB
Adobe PDF
Full metadata record
A new enzyme-free signal amplification-based assay for microRNA (miRNA) detection is developed by using hybridization chain reaction (HCR) coupled with a graphene oxide (GO) surface-anchored fluorescence signal readout pathway. MiRNAs can efficiently initiate HCR between two species of fluorescent hairpin probes. After HCR, both of the excess hairpin probes and the HCR products will be anchored on the GO surface. The fluorescence of the hairpin probes can be completely quenched by GO, whereas the HCR products maintain strong fluorescence. Therefore, integrating HCR strategy for signal amplification with selective fluorescence quenching effects of GO provides a versatile miRNA assay. © 2012 American Chemical Society.
Please use this identifier to cite or link to this item: