Graphene surface-anchored fluorescence sensor for sensitive detection of MicroRNA coupled with enzyme-free signal amplification of hybridization chain reaction

Yang, L; Liu, C; Ren, W; Li, Z

Graphene surface-anchored fluorescence sensor for sensitive detection of MicroRNA coupled with enzyme-free signal amplification of hybridization chain reaction

Yang, L Liu, C Ren, W

Li, Z

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Publication Type:: Journal Article
Citation:: ACS Applied Materials and Interfaces, 2012, 4 (12), pp. 6450 - 6453
Issue Date:: 2012-12-26

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Field	Value	Language
dc.contributor.author	Yang, L	en_US
dc.contributor.author	Liu, C	en_US
dc.contributor.author	Ren, W https://orcid.org/0000-0002-6537-6039	en_US
dc.contributor.author	Li, Z	en_US
dc.date.issued	2012-12-26	en_US
dc.identifier.citation	ACS Applied Materials and Interfaces, 2012, 4 (12), pp. 6450 - 6453	en_US
dc.identifier.issn	1944-8244	en_US
dc.identifier.uri	http://hdl.handle.net/10453/117589
dc.description.abstract	A new enzyme-free signal amplification-based assay for microRNA (miRNA) detection is developed by using hybridization chain reaction (HCR) coupled with a graphene oxide (GO) surface-anchored fluorescence signal readout pathway. MiRNAs can efficiently initiate HCR between two species of fluorescent hairpin probes. After HCR, both of the excess hairpin probes and the HCR products will be anchored on the GO surface. The fluorescence of the hairpin probes can be completely quenched by GO, whereas the HCR products maintain strong fluorescence. Therefore, integrating HCR strategy for signal amplification with selective fluorescence quenching effects of GO provides a versatile miRNA assay. © 2012 American Chemical Society.	en_US
dc.relation.ispartof	ACS Applied Materials and Interfaces	en_US
dc.relation.isbasedon	10.1021/am302268t	en_US
dc.subject.classification	Nanoscience & Nanotechnology	en_US
dc.subject.mesh	Graphite	en_US
dc.subject.mesh	MicroRNAs	en_US
dc.subject.mesh	Nucleic Acid Hybridization	en_US
dc.subject.mesh	Surface Properties	en_US
dc.subject.mesh	Fluorescence	en_US
dc.subject.mesh	Limit of Detection	en_US
dc.title	Graphene surface-anchored fluorescence sensor for sensitive detection of MicroRNA coupled with enzyme-free signal amplification of hybridization chain reaction	en_US
dc.type	Journal Article
utslib.citation.volume	12	en_US
utslib.citation.volume	4	en_US
utslib.for	0303 Macromolecular and Materials Chemistry	en_US
utslib.for	0306 Physical Chemistry (incl. Structural)	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	09 Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access
pubs.issue	12	en_US
pubs.publication-status	Published	en_US
pubs.volume	4	en_US

Abstract:

A new enzyme-free signal amplification-based assay for microRNA (miRNA) detection is developed by using hybridization chain reaction (HCR) coupled with a graphene oxide (GO) surface-anchored fluorescence signal readout pathway. MiRNAs can efficiently initiate HCR between two species of fluorescent hairpin probes. After HCR, both of the excess hairpin probes and the HCR products will be anchored on the GO surface. The fluorescence of the hairpin probes can be completely quenched by GO, whereas the HCR products maintain strong fluorescence. Therefore, integrating HCR strategy for signal amplification with selective fluorescence quenching effects of GO provides a versatile miRNA assay. © 2012 American Chemical Society.

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http://hdl.handle.net/10453/117589