Complete Genome Sequence of the Cystic Fibrosis Pathogen Achromobacter xylosoxidans NH44784-1996 Complies with Important Pathogenic Phenotypes

Jakobsen, TH; Hansen, MA; Jensen, PØ; Hansen, L; Riber, L; Cockburn, A; Kolpen, M; Rønne Hansen, C; Ridderberg, W; Eickhardt, S; Hansen, M; Kerpedjiev, P; Alhede, M; Qvortrup, K; Burmølle, M; Moser, C; Kühl, M; Ciofu, O; Givskov, M; Sørensen, SJ; Høiby, N; Bjarnsholt, T

Complete Genome Sequence of the Cystic Fibrosis Pathogen Achromobacter xylosoxidans NH44784-1996 Complies with Important Pathogenic Phenotypes

Jakobsen, TH Hansen, MA Jensen, PØ Hansen, L Riber, L Cockburn, A Kolpen, M Rønne Hansen, C Ridderberg, W Eickhardt, S Hansen, M Kerpedjiev, P Alhede, M Qvortrup, K Burmølle, M Moser, C Kühl, M

Ciofu, O Givskov, M Sørensen, SJ Høiby, N Bjarnsholt, T

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Publication Type:: Journal Article
Citation:: PLoS ONE, 2013, 8 (7)
Issue Date:: 2013-07-22

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Field	Value	Language
dc.contributor.author	Jakobsen, TH	en_US
dc.contributor.author	Hansen, MA	en_US
dc.contributor.author	Jensen, PØ	en_US
dc.contributor.author	Hansen, L	en_US
dc.contributor.author	Riber, L	en_US
dc.contributor.author	Cockburn, A	en_US
dc.contributor.author	Kolpen, M	en_US
dc.contributor.author	Rønne Hansen, C	en_US
dc.contributor.author	Ridderberg, W	en_US
dc.contributor.author	Eickhardt, S	en_US
dc.contributor.author	Hansen, M	en_US
dc.contributor.author	Kerpedjiev, P	en_US
dc.contributor.author	Alhede, M	en_US
dc.contributor.author	Qvortrup, K	en_US
dc.contributor.author	Burmølle, M	en_US
dc.contributor.author	Moser, C	en_US
dc.contributor.author	Kühl, M https://orcid.org/0000-0002-1792-4790	en_US
dc.contributor.author	Ciofu, O	en_US
dc.contributor.author	Givskov, M	en_US
dc.contributor.author	Sørensen, SJ	en_US
dc.contributor.author	Høiby, N	en_US
dc.contributor.author	Bjarnsholt, T	en_US
dc.date.available	2013-05-29	en_US
dc.date.issued	2013-07-22	en_US
dc.identifier.citation	PLoS ONE, 2013, 8 (7)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/117604
dc.description.abstract	Achromobacter xylosoxidans is an environmental opportunistic pathogen, which infects an increasing number of immunocompromised patients. In this study we combined genomic analysis of a clinical isolated A. xylosoxidans. strain with phenotypic investigations of its important pathogenic features. We present a complete assembly of the genome of A. xylosoxidans NH44784-1996, an isolate from a cystic fibrosis patient obtained in 1996. The genome of in 1996. The genom NH44784-1996 contains approximately 7 million base pairs with 6390 potential protein-coding sequences. We identified several features that render it an opportunistic human pathogen, We found genes involved in anaerobic growth and the pgaABCD operon encoding the biofilm adhesin poly-β-1,6-N-acetyl-D-glucosamin. Furthermore, the genome contains a range of antibiotic resistance genes coding efflux pump systems and antibiotic modifying enzymes. In vitro studies of in 1996. The genom NH44784-1996 confirmed the genomic evidence for its ability to form biofilms, anaerobic growth via denitrification, and resistance to a broad range of antibiotics. Our investigation enables further studies of the functionality of important identified genes contributing to the pathogenicity of in 1996. The genom and thereby improves our understanding and ability to treat this emerging pathogen. © 2013 Jakobsen et al.	en_US
dc.relation.ispartof	PLoS ONE	en_US
dc.relation.isbasedon	10.1371/journal.pone.0068484	en_US
dc.subject.classification	General Science & Technology	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Biofilms	en_US
dc.subject.mesh	Cystic Fibrosis	en_US
dc.subject.mesh	Oxygen	en_US
dc.subject.mesh	beta-Lactamases	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Anti-Bacterial Agents	en_US
dc.subject.mesh	Genomics	en_US
dc.subject.mesh	Bacterial Adhesion	en_US
dc.subject.mesh	Drug Resistance, Bacterial	en_US
dc.subject.mesh	Phenotype	en_US
dc.subject.mesh	Genome, Bacterial	en_US
dc.subject.mesh	Achromobacter denitrificans	en_US
dc.subject.mesh	Denitrification	en_US
dc.subject.mesh	Molecular Sequence Annotation	en_US
dc.title	Complete Genome Sequence of the Cystic Fibrosis Pathogen Achromobacter xylosoxidans NH44784-1996 Complies with Important Pathogenic Phenotypes	en_US
dc.type	Journal Article
utslib.citation.volume	7	en_US
utslib.citation.volume	8	en_US
utslib.for	0604 Genetics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	open_access
pubs.issue	7	en_US
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

Achromobacter xylosoxidans is an environmental opportunistic pathogen, which infects an increasing number of immunocompromised patients. In this study we combined genomic analysis of a clinical isolated A. xylosoxidans. strain with phenotypic investigations of its important pathogenic features. We present a complete assembly of the genome of A. xylosoxidans NH44784-1996, an isolate from a cystic fibrosis patient obtained in 1996. The genome of in 1996. The genom NH44784-1996 contains approximately 7 million base pairs with 6390 potential protein-coding sequences. We identified several features that render it an opportunistic human pathogen, We found genes involved in anaerobic growth and the pgaABCD operon encoding the biofilm adhesin poly-β-1,6-N-acetyl-D-glucosamin. Furthermore, the genome contains a range of antibiotic resistance genes coding efflux pump systems and antibiotic modifying enzymes. In vitro studies of in 1996. The genom NH44784-1996 confirmed the genomic evidence for its ability to form biofilms, anaerobic growth via denitrification, and resistance to a broad range of antibiotics. Our investigation enables further studies of the functionality of important identified genes contributing to the pathogenicity of in 1996. The genom and thereby improves our understanding and ability to treat this emerging pathogen. © 2013 Jakobsen et al.

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http://hdl.handle.net/10453/117604