The role of planktonic Flavobacteria in processing algal organic matter in coastal East Antarctica revealed using metagenomics and metaproteomics

Williams, TJ; Wilkins, D; Long, E; Evans, F; Demaere, MZ; Raftery, MJ; Cavicchioli, R

The role of planktonic Flavobacteria in processing algal organic matter in coastal East Antarctica revealed using metagenomics and metaproteomics

Williams, TJ Wilkins, D Long, E Evans, F Demaere, MZ

Raftery, MJ Cavicchioli, R

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Publication Type:: Journal Article
Citation:: Environmental Microbiology, 2013, 15 (5), pp. 1302 - 1317
Issue Date:: 2013-05-01

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Field	Value	Language
dc.contributor.author	Williams, TJ	en_US
dc.contributor.author	Wilkins, D	en_US
dc.contributor.author	Long, E	en_US
dc.contributor.author	Evans, F	en_US
dc.contributor.author	Demaere, MZ https://orcid.org/0000-0002-7601-5108	en_US
dc.contributor.author	Raftery, MJ	en_US
dc.contributor.author	Cavicchioli, R	en_US
dc.date.available	2012-09-26	en_US
dc.date.issued	2013-05-01	en_US
dc.identifier.citation	Environmental Microbiology, 2013, 15 (5), pp. 1302 - 1317	en_US
dc.identifier.issn	1462-2912	en_US
dc.identifier.uri	http://hdl.handle.net/10453/117634
dc.description.abstract	Heterotrophic marine bacteria play key roles in remineralizing organic matter generated from primary production. However, far more is known about which groups are dominant than about the cellular processes they perform in order to become dominant. In the Southern Ocean, eukaryotic phytoplankton are the dominant primary producers. In this study we used metagenomics and metaproteomics to determine how the dominant bacterial and archaeal plankton processed bloom material. We examined the microbial community composition in 14 metagenomes and found that the relative abundance of Flavobacteria (dominated by Polaribacter) was positively correlated with chlorophyll a fluorescence, and the relative abundance of SAR11 was inversely correlated with both fluorescence and Flavobacteria abundance. By performing metaproteomics on the sample with the highest relative abundance of Flavobacteria (Newcomb Bay, East Antarctica) we defined how Flavobacteria attach to and degrade diverse complex organic material, how they make labile compounds available to Alphaproteobacteria (especially SAR11) and Gammaproteobacteria, and how these heterotrophic Proteobacteria target and utilize these nutrients. The presence of methylotrophic proteins for archaea and bacteria also indicated the importance of metabolic specialists. Overall, the study provides functional data for the microbial mechanisms of nutrient cycling at the surface of the coastal Southern Ocean. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.	en_US
dc.relation.ispartof	Environmental Microbiology	en_US
dc.relation.isbasedon	10.1111/1462-2920.12017	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Plankton	en_US
dc.subject.mesh	Phytoplankton	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	Flavobacteriaceae	en_US
dc.subject.mesh	Proteobacteria	en_US
dc.subject.mesh	Archaea	en_US
dc.subject.mesh	Chlorophyll	en_US
dc.subject.mesh	Proteomics	en_US
dc.subject.mesh	Biodiversity	en_US
dc.subject.mesh	Seawater	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Antarctic Regions	en_US
dc.subject.mesh	Heterotrophic Processes	en_US
dc.subject.mesh	Metagenomics	en_US
dc.subject.mesh	Eukaryota	en_US
dc.subject.mesh	Chlorophyll A	en_US
dc.title	The role of planktonic Flavobacteria in processing algal organic matter in coastal East Antarctica revealed using metagenomics and metaproteomics	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	15	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0603 Evolutionary Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	15	en_US

Abstract:

Heterotrophic marine bacteria play key roles in remineralizing organic matter generated from primary production. However, far more is known about which groups are dominant than about the cellular processes they perform in order to become dominant. In the Southern Ocean, eukaryotic phytoplankton are the dominant primary producers. In this study we used metagenomics and metaproteomics to determine how the dominant bacterial and archaeal plankton processed bloom material. We examined the microbial community composition in 14 metagenomes and found that the relative abundance of Flavobacteria (dominated by Polaribacter) was positively correlated with chlorophyll a fluorescence, and the relative abundance of SAR11 was inversely correlated with both fluorescence and Flavobacteria abundance. By performing metaproteomics on the sample with the highest relative abundance of Flavobacteria (Newcomb Bay, East Antarctica) we defined how Flavobacteria attach to and degrade diverse complex organic material, how they make labile compounds available to Alphaproteobacteria (especially SAR11) and Gammaproteobacteria, and how these heterotrophic Proteobacteria target and utilize these nutrients. The presence of methylotrophic proteins for archaea and bacteria also indicated the importance of metabolic specialists. Overall, the study provides functional data for the microbial mechanisms of nutrient cycling at the surface of the coastal Southern Ocean. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.

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http://hdl.handle.net/10453/117634