How to build a time-gated luminescence microscope

John Wiley & Sons
Publication Type:
Current Protocols in Cytometry, 2014
Issue Date:
Full metadata record
Files in This Item:
Filename Description Size
Rajendran CurrProtCyto 2013 (1).pdfPublished Version2.66 MB
Adobe PDF
The sensitivity of filter-based fluorescence microscopy techniques is limited by autofluorescence background. Time-gated detection is a practical way to suppress autofluorescence, enabling higher contrast and improved sensitivity. In the past few years, three groups of authors have demonstrated independent approaches to build robust versions of time-gated luminescence microscopes. Three detailed, step-by-step protocols are provided here for modifying standard fluorescent microscopes to permit imaging time-gated luminescence
Please use this identifier to cite or link to this item: