How to build a time-gated luminescence microscope.

Publisher:
Wiley-Blackwell
Publication Type:
Journal Article
Citation:
Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.], 2014, 67 pp. Unit - 2.22.
Issue Date:
2014-01-02
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The sensitivity of filter-based fluorescence microscopy techniques is limited by autofluorescence background. Time-gated detection is a practical way to suppress autofluorescence, enabling higher contrast and improved sensitivity. In the past few years, three groups of authors have demonstrated independent approaches to build robust versions of time-gated luminescence microscopes. Three detailed, step-by-step protocols are provided here for modifying standard fluorescent microscopes to permit imaging time-gated luminescence.
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