How to build a time-gated luminescence microscope

Publication Type:
Issue Date:
Filename Description Size
Rajendran CurrProtCyto 2013 (1).pdfPublished Version2.66 MB
Adobe PDF
Full metadata record
The sensitivity of filter-based fluorescence microscopy techniques is limited by autofluorescence background. Time-gated detection is a practical way to suppress autofluorescence, enabling higher contrast and improved sensitivity. In the past few years, three groups of authors have demonstrated independent approaches to build robust versions of time-gated luminescence microscopes. Three detailed, step-by-step protocols are provided here for modifying standard fluorescent microscopes to permit imaging time-gated luminescence. © 2014 by John Wiley & Sons, Inc.
Please use this identifier to cite or link to this item: