How to build a time-gated luminescence microscope

Publication Type:
Chapter
Citation:
2014
Issue Date:
2014-01-01
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The sensitivity of filter-based fluorescence microscopy techniques is limited by autofluorescence background. Time-gated detection is a practical way to suppress autofluorescence, enabling higher contrast and improved sensitivity. In the past few years, three groups of authors have demonstrated independent approaches to build robust versions of time-gated luminescence microscopes. Three detailed, step-by-step protocols are provided here for modifying standard fluorescent microscopes to permit imaging time-gated luminescence. © 2014 by John Wiley & Sons, Inc.
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