Microbial ecology of an Antarctic hypersaline lake: Genomic assessment of ecophysiology among dominant haloarchaea

Williams, TJ; Allen, MA; Demaere, MZ; Kyrpides, NC; Tringe, SG; Woyke, T; Cavicchioli, R

Microbial ecology of an Antarctic hypersaline lake: Genomic assessment of ecophysiology among dominant haloarchaea

Williams, TJ Allen, MA Demaere, MZ

Kyrpides, NC Tringe, SG Woyke, T Cavicchioli, R

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Publication Type:: Journal Article
Citation:: ISME Journal, 2014, 8 (8), pp. 1645 - 1658
Issue Date:: 2014-01-01

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Field	Value	Language
dc.contributor.author	Williams, TJ	en_US
dc.contributor.author	Allen, MA	en_US
dc.contributor.author	Demaere, MZ https://orcid.org/0000-0002-7601-5108	en_US
dc.contributor.author	Kyrpides, NC	en_US
dc.contributor.author	Tringe, SG	en_US
dc.contributor.author	Woyke, T	en_US
dc.contributor.author	Cavicchioli, R	en_US
dc.date.available	2014-01-17	en_US
dc.date.issued	2014-01-01	en_US
dc.identifier.citation	ISME Journal, 2014, 8 (8), pp. 1645 - 1658	en_US
dc.identifier.issn	1751-7362	en_US
dc.identifier.uri	http://hdl.handle.net/10453/117785
dc.description.abstract	Deep Lake in Antarctica is a cold, hypersaline system where four types of haloarchaea representing distinct genera comprise >70% of the lake community: strain tADL ∼44%, strain DL31 ∼18%, Halorubrum lacusprofundi ∼10% and strain DL1 ∼0.3%. By performing comparative genomics, growth substrate assays, and analyses of distribution by lake depth, size partitioning and lake nutrient composition, we were able to infer important metabolic traits and ecophysiological characteristics of the four Antarctic haloarchaea that contribute to their hierarchical persistence and coexistence in Deep Lake. tADL is characterized by a capacity for motility via flagella (archaella) and gas vesicles, a highly saccharolytic metabolism, a preference for glycerol, and photoheterotrophic growth. In contrast, DL31 has a metabolism specialized in processing proteins and peptides, and appears to prefer an association with particulate organic matter, while lacking the genomic potential for motility. H. lacusprofundi is the least specialized, displaying a genomic potential for the utilization of diverse organic substrates. The least abundant species, DL1, is characterized by a preference for catabolism of amino acids, and is the only one species that lacks genes needed for glycerol degradation. Despite the four haloarchaea being distributed throughout the water column, our analyses describe a range of distinctive features, including preferences for substrates that are indicative of ecological niche partitioning. The individual characteristics could be responsible for shaping the composition of the haloarchaeal community throughout the lake by enabling selection of ecotypes and maintaining sympatric speciation. © 2014 International Society for Microbial Ecology All rights reserved.	en_US
dc.relation.ispartof	ISME Journal	en_US
dc.relation.isbasedon	10.1038/ismej.2014.18	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Halobacteriaceae	en_US
dc.subject.mesh	Nitrogen	en_US
dc.subject.mesh	ATP-Binding Cassette Transporters	en_US
dc.subject.mesh	Genomics	en_US
dc.subject.mesh	Genome, Archaeal	en_US
dc.subject.mesh	Antarctic Regions	en_US
dc.subject.mesh	Carbohydrate Metabolism	en_US
dc.subject.mesh	Salinity	en_US
dc.subject.mesh	Lakes	en_US
dc.subject.mesh	Ecotype	en_US
dc.title	Microbial ecology of an Antarctic hypersaline lake: Genomic assessment of ecophysiology among dominant haloarchaea	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
utslib.for	060114 Systems Biology	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	10 Technology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	8	en_US
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

Deep Lake in Antarctica is a cold, hypersaline system where four types of haloarchaea representing distinct genera comprise >70% of the lake community: strain tADL ∼44%, strain DL31 ∼18%, Halorubrum lacusprofundi ∼10% and strain DL1 ∼0.3%. By performing comparative genomics, growth substrate assays, and analyses of distribution by lake depth, size partitioning and lake nutrient composition, we were able to infer important metabolic traits and ecophysiological characteristics of the four Antarctic haloarchaea that contribute to their hierarchical persistence and coexistence in Deep Lake. tADL is characterized by a capacity for motility via flagella (archaella) and gas vesicles, a highly saccharolytic metabolism, a preference for glycerol, and photoheterotrophic growth. In contrast, DL31 has a metabolism specialized in processing proteins and peptides, and appears to prefer an association with particulate organic matter, while lacking the genomic potential for motility. H. lacusprofundi is the least specialized, displaying a genomic potential for the utilization of diverse organic substrates. The least abundant species, DL1, is characterized by a preference for catabolism of amino acids, and is the only one species that lacks genes needed for glycerol degradation. Despite the four haloarchaea being distributed throughout the water column, our analyses describe a range of distinctive features, including preferences for substrates that are indicative of ecological niche partitioning. The individual characteristics could be responsible for shaping the composition of the haloarchaeal community throughout the lake by enabling selection of ecotypes and maintaining sympatric speciation. © 2014 International Society for Microbial Ecology All rights reserved.

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http://hdl.handle.net/10453/117785