A novel amperometric hydrogen peroxide biosensor based on electrospun Hb-collagen composite

Guo, F; Xu, XX; Sun, ZZ; Zhang, JX; Meng, ZX; Zheng, W; Zhou, HM; Wang, BL; Zheng, YF

A novel amperometric hydrogen peroxide biosensor based on electrospun Hb-collagen composite

Guo, F Xu, XX

Sun, ZZ Zhang, JX Meng, ZX Zheng, W Zhou, HM Wang, BL Zheng, YF

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Publication Type:: Journal Article
Citation:: Colloids and Surfaces B: Biointerfaces, 2011, 86 (1), pp. 140 - 145
Issue Date:: 2011-08-01

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Field	Value	Language
dc.contributor.author	Guo, F	en_US
dc.contributor.author	Xu, XX https://orcid.org/0000-0002-2598-3766	en_US
dc.contributor.author	Sun, ZZ	en_US
dc.contributor.author	Zhang, JX	en_US
dc.contributor.author	Meng, ZX	en_US
dc.contributor.author	Zheng, W	en_US
dc.contributor.author	Zhou, HM	en_US
dc.contributor.author	Wang, BL	en_US
dc.contributor.author	Zheng, YF	en_US
dc.date.available	2011-03-22	en_US
dc.date.issued	2011-08-01	en_US
dc.identifier.citation	Colloids and Surfaces B: Biointerfaces, 2011, 86 (1), pp. 140 - 145	en_US
dc.identifier.issn	0927-7765	en_US
dc.identifier.uri	http://hdl.handle.net/10453/117928
dc.description.abstract	In this paper, the hemoglobin (Hb)-collagen microbelt modified electrode with three-dimensional configuration was fabricated via the electrospinning method. Direct electron transfer of the Hb immobilized into the electrospun collagen microbelts was greatly facilitated. The apparent heterogeneous electron transfer rate constant (ks) was calculated to be 270.6s-1. The electrospun Hb-collagen microbelt modified electrode showed an excellent bioelectrocatalytic activity toward the reduction of H2O2. The amperometric response of the biosensor varied linearly with the H2O2 concentration ranging from 5×10-6molL-1 to 30×10-6molL-1, with a detection limit of 0.37×10-6molL-1 (signal-to-noise ratio of 3). The apparent Michaelis-Menten constant (Kmapp) was 77.7μmolL-1. The established biosensor exhibited fast amperometric response, high sensitivity, good reproducibility and stability. © 2011 Elsevier B.V.	en_US
dc.relation.ispartof	Colloids and Surfaces B: Biointerfaces	en_US
dc.relation.isbasedon	10.1016/j.colsurfb.2011.03.032	en_US
dc.subject.classification	Chemical Physics	en_US
dc.subject.mesh	Hydrogen Peroxide	en_US
dc.subject.mesh	Collagen	en_US
dc.subject.mesh	Hemoglobins	en_US
dc.subject.mesh	Biosensing Techniques	en_US
dc.subject.mesh	Electrochemistry	en_US
dc.title	A novel amperometric hydrogen peroxide biosensor based on electrospun Hb-collagen composite	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	86	en_US
utslib.for	0306 Physical Chemistry (incl. Structural)	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	0904 Chemical Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	86	en_US

Abstract:

In this paper, the hemoglobin (Hb)-collagen microbelt modified electrode with three-dimensional configuration was fabricated via the electrospinning method. Direct electron transfer of the Hb immobilized into the electrospun collagen microbelts was greatly facilitated. The apparent heterogeneous electron transfer rate constant (ks) was calculated to be 270.6s-1. The electrospun Hb-collagen microbelt modified electrode showed an excellent bioelectrocatalytic activity toward the reduction of H2O2. The amperometric response of the biosensor varied linearly with the H2O2 concentration ranging from 5×10-6molL-1 to 30×10-6molL-1, with a detection limit of 0.37×10-6molL-1 (signal-to-noise ratio of 3). The apparent Michaelis-Menten constant (Kmapp) was 77.7μmolL-1. The established biosensor exhibited fast amperometric response, high sensitivity, good reproducibility and stability. © 2011 Elsevier B.V.

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http://hdl.handle.net/10453/117928