A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite

Xu, XX; Zhang, JX; Guo, F; Zheng, W; Zhou, HM; Wang, BL; Zheng, YF; Wang, YB; Cheng, Y; Lou, X; Jang, BZ

A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite

Xu, XX

Zhang, JX Guo, F Zheng, W Zhou, HM Wang, BL Zheng, YF Wang, YB Cheng, Y Lou, X Jang, BZ

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Publication Type:: Journal Article
Citation:: Colloids and Surfaces B: Biointerfaces, 2011, 84 (2), pp. 427 - 432
Issue Date:: 2011-06-01

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Field	Value	Language
dc.contributor.author	Xu, XX https://orcid.org/0000-0002-2598-3766	en_US
dc.contributor.author	Zhang, JX	en_US
dc.contributor.author	Guo, F	en_US
dc.contributor.author	Zheng, W	en_US
dc.contributor.author	Zhou, HM	en_US
dc.contributor.author	Wang, BL	en_US
dc.contributor.author	Zheng, YF	en_US
dc.contributor.author	Wang, YB	en_US
dc.contributor.author	Cheng, Y	en_US
dc.contributor.author	Lou, X	en_US
dc.contributor.author	Jang, BZ	en_US
dc.date.available	2011-01-23	en_US
dc.date.issued	2011-06-01	en_US
dc.identifier.citation	Colloids and Surfaces B: Biointerfaces, 2011, 84 (2), pp. 427 - 432	en_US
dc.identifier.issn	0927-7765	en_US
dc.identifier.uri	http://hdl.handle.net/10453/117930
dc.description.abstract	In this paper, an amperometric biosensor of hydrogen peroxide (H2O2) was fabricated by immobilization of Hemoglobin (Hb) on a Pluronic P123-nanographene platelet (NGP) composite. Direct electron transfer in the Hb-immobilized P123-NGP composite film was greatly facilitated. The surface concentration (Γ) and apparent heterogeneous electron transfer rate constant (ks) were calculated to be (1.60±0.17)×10-10molcm-2 and 48.51s-1, respectively. In addition, the Hb/Pluronic P123-NGP composite showed excellent bioelectrocatalytic activity toward the reduction of H2O2. The biosensor of H2O2 exhibited a linear response to H2O2 in the range of 10-150μM and a detection limit of 8.24μM (S/N=3) was obtained. The apparent Michaelis-Menten constant (Kmapp) was 45.35μM. The resulting biosensor showed fast amperometric response, with very high sensitivity, reliability and effectiveness. © 2011 Elsevier B.V.	en_US
dc.relation.ispartof	Colloids and Surfaces B: Biointerfaces	en_US
dc.relation.isbasedon	10.1016/j.colsurfb.2011.01.037	en_US
dc.subject.classification	Chemical Physics	en_US
dc.subject.mesh	Hydrogen Peroxide	en_US
dc.subject.mesh	Graphite	en_US
dc.subject.mesh	Poloxalene	en_US
dc.subject.mesh	Hemoglobins	en_US
dc.subject.mesh	Microscopy, Electron, Transmission	en_US
dc.subject.mesh	Biosensing Techniques	en_US
dc.subject.mesh	Surface Properties	en_US
dc.subject.mesh	Nanostructures	en_US
dc.subject.mesh	Immobilized Proteins	en_US
dc.title	A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	84	en_US
utslib.for	0306 Physical Chemistry (incl. Structural)	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	0904 Chemical Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	84	en_US

Abstract:

In this paper, an amperometric biosensor of hydrogen peroxide (H2O2) was fabricated by immobilization of Hemoglobin (Hb) on a Pluronic P123-nanographene platelet (NGP) composite. Direct electron transfer in the Hb-immobilized P123-NGP composite film was greatly facilitated. The surface concentration (Γ) and apparent heterogeneous electron transfer rate constant (ks) were calculated to be (1.60±0.17)×10-10molcm-2 and 48.51s-1, respectively. In addition, the Hb/Pluronic P123-NGP composite showed excellent bioelectrocatalytic activity toward the reduction of H2O2. The biosensor of H2O2 exhibited a linear response to H2O2 in the range of 10-150μM and a detection limit of 8.24μM (S/N=3) was obtained. The apparent Michaelis-Menten constant (Kmapp) was 45.35μM. The resulting biosensor showed fast amperometric response, with very high sensitivity, reliability and effectiveness. © 2011 Elsevier B.V.

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http://hdl.handle.net/10453/117930