Spirometry filters can be used to detect exhaled respiratory viruses

Mitchell, AB; Mourad, B; Tovey, E; Buddle, L; Peters, M; Morgan, L; Oliver, BG

Spirometry filters can be used to detect exhaled respiratory viruses

Mitchell, AB

Mourad, B Tovey, E Buddle, L Peters, M Morgan, L Oliver, BG

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Publication Type:: Journal Article
Citation:: Journal of Breath Research, 2016, 10 (4)
Issue Date:: 2016-09-26

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Field	Value	Language
dc.contributor.author	Mitchell, AB https://orcid.org/0000-0002-9086-1589	en_US
dc.contributor.author	Mourad, B	en_US
dc.contributor.author	Tovey, E	en_US
dc.contributor.author	Buddle, L	en_US
dc.contributor.author	Peters, M	en_US
dc.contributor.author	Morgan, L	en_US
dc.contributor.author	Oliver, BG https://orcid.org/0000-0002-7122-9262	en_US
dc.date.issued	2016-09-26	en_US
dc.identifier.citation	Journal of Breath Research, 2016, 10 (4)	en_US
dc.identifier.issn	1752-7155	en_US
dc.identifier.uri	http://hdl.handle.net/10453/117947
dc.description.abstract	© 2016 IOP Publishing Ltd. Respiratory viruses are very common in the community and contribute to the burden of illness for patients with chronic respiratory diseases, including acute exacerbations. Traditional sampling methods are invasive and problematic to repeat. Accordingly, we explored whether respiratory viruses could be isolated from disposable spirometry filters and whether detection of viruses in this context represented presence in the upper or lower respiratory tract. Discovery (n = 53) and validation (n = 49) cohorts were recruited from a hospital outpatient department during two different time periods. Spirometry mouthpiece filters were collected from all participants. Respiratory secretions were sampled from the upper and lower respiratory tract by nasal washing (NW), sputum, and bronchoalveolar lavage (BAL). All samples were examined using RT-PCR to identify a panel of respiratory viruses (rhinovirus, respiratory syncytial virus, influenza A, influenza B, parainfluenza virus 1, 2 & 3, and human metapneumovirus). Rhinovirus was quantified using qPCR. Paired filter-NW samples (n = 29), filter-sputum samples (n = 24), filter-BAL samples (n = 39) and filter-NW-BAL samples (n = 10) provided a range of comparisons. At least one virus was detected in any sample in 85% of participants in the discovery cohort versus 45% in the validation cohort. Overall, 72% of viruses identified in the paired comparator method matched those detected in spirometry filters. There was a high correlation between viruses identified in spirometry filters compared with viruses identified in both the upper and lower respiratory tract using traditional sampling methods. Our results suggest that examination of spirometry filters may be a novel and inexpensive sampling method for the presence of respiratory viruses in exhaled breath.	en_US
dc.relation	http://purl.org/au-research/grants/nhmrc/APP1026880
dc.relation	http://purl.org/au-research/grants/nhmrc/APP1110368
dc.relation.ispartof	Journal of Breath Research	en_US
dc.relation.isbasedon	10.1088/1752-7155/10/4/046002	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Viruses	en_US
dc.subject.mesh	Respiratory Tract Infections	en_US
dc.subject.mesh	Common Cold	en_US
dc.subject.mesh	Breath Tests	en_US
dc.subject.mesh	Spirometry	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Cohort Studies	en_US
dc.subject.mesh	Reproducibility of Results	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Exhalation	en_US
dc.subject.mesh	Adult	en_US
dc.subject.mesh	Aged	en_US
dc.subject.mesh	Aged, 80 and over	en_US
dc.subject.mesh	Middle Aged	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Surveys and Questionnaires	en_US
dc.title	Spirometry filters can be used to detect exhaled respiratory viruses	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	10	en_US
utslib.for	1108 Medical Microbiology	en_US
utslib.for	0903 Biomedical Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	open_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	10	en_US

Abstract:

© 2016 IOP Publishing Ltd. Respiratory viruses are very common in the community and contribute to the burden of illness for patients with chronic respiratory diseases, including acute exacerbations. Traditional sampling methods are invasive and problematic to repeat. Accordingly, we explored whether respiratory viruses could be isolated from disposable spirometry filters and whether detection of viruses in this context represented presence in the upper or lower respiratory tract. Discovery (n = 53) and validation (n = 49) cohorts were recruited from a hospital outpatient department during two different time periods. Spirometry mouthpiece filters were collected from all participants. Respiratory secretions were sampled from the upper and lower respiratory tract by nasal washing (NW), sputum, and bronchoalveolar lavage (BAL). All samples were examined using RT-PCR to identify a panel of respiratory viruses (rhinovirus, respiratory syncytial virus, influenza A, influenza B, parainfluenza virus 1, 2 & 3, and human metapneumovirus). Rhinovirus was quantified using qPCR. Paired filter-NW samples (n = 29), filter-sputum samples (n = 24), filter-BAL samples (n = 39) and filter-NW-BAL samples (n = 10) provided a range of comparisons. At least one virus was detected in any sample in 85% of participants in the discovery cohort versus 45% in the validation cohort. Overall, 72% of viruses identified in the paired comparator method matched those detected in spirometry filters. There was a high correlation between viruses identified in spirometry filters compared with viruses identified in both the upper and lower respiratory tract using traditional sampling methods. Our results suggest that examination of spirometry filters may be a novel and inexpensive sampling method for the presence of respiratory viruses in exhaled breath.

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http://hdl.handle.net/10453/117947