Detection of Dientamoeba fragilis in animal faeces using species specific real time PCR assay

Chan, D; Barratt, J; Roberts, T; Phillips, O; Šlapeta, J; Ryan, U; Marriott, D; Harkness, J; Ellis, J; Stark, D

Detection of Dientamoeba fragilis in animal faeces using species specific real time PCR assay

Chan, D Barratt, J

Roberts, T Phillips, O Šlapeta, J Ryan, U Marriott, D Harkness, J Ellis, J

Stark, D

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Publication Type:: Journal Article
Citation:: Veterinary Parasitology, 2016, 227 pp. 42 - 47
Issue Date:: 2016-08-30

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Field	Value	Language
dc.contributor.author	Chan, D	en_US
dc.contributor.author	Barratt, J https://orcid.org/0000-0001-8711-2408	en_US
dc.contributor.author	Roberts, T	en_US
dc.contributor.author	Phillips, O	en_US
dc.contributor.author	Šlapeta, J	en_US
dc.contributor.author	Ryan, U	en_US
dc.contributor.author	Marriott, D	en_US
dc.contributor.author	Harkness, J	en_US
dc.contributor.author	Ellis, J https://orcid.org/0000-0001-7328-4831	en_US
dc.contributor.author	Stark, D	en_US
dc.date.available	2016-07-19	en_US
dc.date.issued	2016-08-30	en_US
dc.identifier.citation	Veterinary Parasitology, 2016, 227 pp. 42 - 47	en_US
dc.identifier.issn	0304-4017	en_US
dc.identifier.uri	http://hdl.handle.net/10453/117962
dc.description.abstract	© 2016 Elsevier B.V. Dientamoeba fragilis is a potentially pathogenic, enteric, protozoan parasite with a worldwide distribution. While clinical case reports and prevalence studies appear regularly in the scientific literature, little attention has been paid to this parasite's biology, life cycle, host range, and possible transmission routes. Overall, these aspects of Dientamoeba biology remain poorly understood at best. In this study, a total of 420 animal samples, collected from Australia, were surveyed for the presence of Dientamoeba fragilis using PCR. Several PCR assays were evaluated for sensitivity and specificity. Two previously published PCR methods demonstrated cross reactivity with other trichomonads commonly found in animal samples. Only one assay exhibited excellent specificity. Using this assay D. fragilis was detected from one dog and one cat sample. This is the first report of D. fragilis from these animals and highlights the role companion animals may play in D. fragilis transmission. This study demonstrated that some published D. fragilis molecular assays cross react with other closely related trichomonads and consequently are not suitable for animal prevalence studies.	en_US
dc.relation.ispartof	Veterinary Parasitology	en_US
dc.relation.isbasedon	10.1016/j.vetpar.2016.07.025	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Feces	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Dogs	en_US
dc.subject.mesh	Cats	en_US
dc.subject.mesh	Dientamoeba	en_US
dc.subject.mesh	Cat Diseases	en_US
dc.subject.mesh	Dog Diseases	en_US
dc.subject.mesh	DNA, Protozoan	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Species Specificity	en_US
dc.subject.mesh	Real-Time Polymerase Chain Reaction	en_US
dc.title	Detection of Dientamoeba fragilis in animal faeces using species specific real time PCR assay	en_US
dc.type	Journal Article
utslib.citation.volume	227	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0704 Fisheries Sciences	en_US
utslib.for	0707 Veterinary Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	227	en_US

Abstract:

© 2016 Elsevier B.V. Dientamoeba fragilis is a potentially pathogenic, enteric, protozoan parasite with a worldwide distribution. While clinical case reports and prevalence studies appear regularly in the scientific literature, little attention has been paid to this parasite's biology, life cycle, host range, and possible transmission routes. Overall, these aspects of Dientamoeba biology remain poorly understood at best. In this study, a total of 420 animal samples, collected from Australia, were surveyed for the presence of Dientamoeba fragilis using PCR. Several PCR assays were evaluated for sensitivity and specificity. Two previously published PCR methods demonstrated cross reactivity with other trichomonads commonly found in animal samples. Only one assay exhibited excellent specificity. Using this assay D. fragilis was detected from one dog and one cat sample. This is the first report of D. fragilis from these animals and highlights the role companion animals may play in D. fragilis transmission. This study demonstrated that some published D. fragilis molecular assays cross react with other closely related trichomonads and consequently are not suitable for animal prevalence studies.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/117962