Molecular and phylogenetic characterization of Ostreopsis (Dinophyceae) and the description of a new species, Ostreopsis rhodesae sp. nov., from a subtropical Australian lagoon

Verma, A; Hoppenrath, M; Dorantes-Aranda, JJ; Harwood, DT; Murray, SA

Molecular and phylogenetic characterization of Ostreopsis (Dinophyceae) and the description of a new species, Ostreopsis rhodesae sp. nov., from a subtropical Australian lagoon

Verma, A

Hoppenrath, M Dorantes-Aranda, JJ Harwood, DT Murray, SA

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Publication Type:: Journal Article
Citation:: Harmful Algae, 2016, 60 pp. 116 - 130
Issue Date:: 2016-12-01

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Field	Value	Language
dc.contributor.author	Verma, A https://orcid.org/0000-0003-0119-9915	en_US
dc.contributor.author	Hoppenrath, M	en_US
dc.contributor.author	Dorantes-Aranda, JJ	en_US
dc.contributor.author	Harwood, DT	en_US
dc.contributor.author	Murray, SA https://orcid.org/0000-0001-7096-1307	en_US
dc.date.available	2016-11-10	en_US
dc.date.issued	2016-12-01	en_US
dc.identifier.citation	Harmful Algae, 2016, 60 pp. 116 - 130	en_US
dc.identifier.issn	1568-9883	en_US
dc.identifier.uri	http://hdl.handle.net/10453/117971
dc.description.abstract	© 2016 Elsevier B.V. Cryptic and pseudo-cryptic species are common amongst marine phytoplankton, and may cause misleading inferences of ecological and physiological data of plankton community studies. Deciphering the diversity and distribution of species of the benthic dinoflagellate Ostreopsis is one example, as there are many morphologically indistinct clades that differ greatly genetically and toxicologically from one another. In this study, a new species, Ostreopsis rhodesae from the southern Great Barrier Reef was described. While it initially appeared to be highly similar to several other Ostreopsis species, we found O. rhodesae can be distinguished based on the relative size of the second apical plate (2′), which is twice as long as the APC plate, and separates the third apical (3′) from the third precingular (3′′) plate. Phylogenetic trees based on the SSU, ITS/5.8S and D1-D2 and D8-D10 regions of the LSU rRNA were well supported, and showed a clear difference to other Ostreopsis clades. Compensatory base changes (CBCs) were identified in helices of the ITS2 between O. rhodesae and O. cf. ovata and O. cf. siamensis, which were also present in the same habitat. Fish gill cell lines were toxic to O. rhodesae, cell extracts but no palytoxin-like analogues were found in them. The findings highlight a case of pseudo-cryptic speciation, found in sympatry with closely related and morphologically similar species, but biologically and functionally distinct.	en_US
dc.relation.ispartof	Harmful Algae	en_US
dc.relation.isbasedon	10.1016/j.hal.2016.11.004	en_US
dc.subject.classification	Marine Biology & Hydrobiology	en_US
dc.subject.mesh	Cell Line	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Fishes	en_US
dc.subject.mesh	Dinoflagellida	en_US
dc.subject.mesh	DNA, Ribosomal	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Species Specificity	en_US
dc.subject.mesh	Australia	en_US
dc.title	Molecular and phylogenetic characterization of Ostreopsis (Dinophyceae) and the description of a new species, Ostreopsis rhodesae sp. nov., from a subtropical Australian lagoon	en_US
dc.type	Journal Article
utslib.citation.volume	60	en_US
utslib.for	060405 Gene Expression (incl. Microarray and other genome-wide approaches)	en_US
utslib.for	060409 Molecular Evolution	en_US
utslib.for	060701 Phycology (incl. Marine Grasses)	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	60	en_US

Abstract:

© 2016 Elsevier B.V. Cryptic and pseudo-cryptic species are common amongst marine phytoplankton, and may cause misleading inferences of ecological and physiological data of plankton community studies. Deciphering the diversity and distribution of species of the benthic dinoflagellate Ostreopsis is one example, as there are many morphologically indistinct clades that differ greatly genetically and toxicologically from one another. In this study, a new species, Ostreopsis rhodesae from the southern Great Barrier Reef was described. While it initially appeared to be highly similar to several other Ostreopsis species, we found O. rhodesae can be distinguished based on the relative size of the second apical plate (2′), which is twice as long as the APC plate, and separates the third apical (3′) from the third precingular (3′′) plate. Phylogenetic trees based on the SSU, ITS/5.8S and D1-D2 and D8-D10 regions of the LSU rRNA were well supported, and showed a clear difference to other Ostreopsis clades. Compensatory base changes (CBCs) were identified in helices of the ITS2 between O. rhodesae and O. cf. ovata and O. cf. siamensis, which were also present in the same habitat. Fish gill cell lines were toxic to O. rhodesae, cell extracts but no palytoxin-like analogues were found in them. The findings highlight a case of pseudo-cryptic speciation, found in sympatry with closely related and morphologically similar species, but biologically and functionally distinct.

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http://hdl.handle.net/10453/117971