Evaluation of multi-target immunogenic reagents for the detection of latent and body fluid-contaminated fingermarks

Lam, R; Hofstetter, O; Lennard, C; Roux, C; Spindler, X

Evaluation of multi-target immunogenic reagents for the detection of latent and body fluid-contaminated fingermarks

Lam, R Hofstetter, O Lennard, C Roux, C

Spindler, X

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Publication Type:: Journal Article
Citation:: Forensic Science International, 2016, 264 pp. 168 - 175
Issue Date:: 2016-01-01

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Field	Value	Language
dc.contributor.author	Lam, R	en_US
dc.contributor.author	Hofstetter, O	en_US
dc.contributor.author	Lennard, C	en_US
dc.contributor.author	Roux, C https://orcid.org/0000-0003-3610-420X	en_US
dc.contributor.author	Spindler, X https://orcid.org/0000-0001-6379-351X	en_US
dc.date.available	2016-04-10	en_US
dc.date.issued	2016-01-01	en_US
dc.identifier.citation	Forensic Science International, 2016, 264 pp. 168 - 175	en_US
dc.identifier.issn	0379-0738	en_US
dc.identifier.uri	http://hdl.handle.net/10453/117980
dc.description.abstract	© 2016 Elsevier Ireland Ltd Fingermark enhancement reagents capable of molecular recognition offer a highly selective and sensitive method of detection. Antibodies and aptamers provide a high degree of adaptability for visualisation, allowing for the selection of the most appropriate visualisation wavelength for a particular substrate without the need for specialist equipment or image processing. However, the major hurdle to overcome is the balance between sensitivity and selectivity. Single-target molecular recognition is highly specific, purported to have better detection limits than chemical reactions or stains, and can provide information about the donor or activity, but often results in incomplete ridge pattern development. Consequently, the development and evaluation of multi-target biomolecular reagents for fingermark enhancement was investigated, with the focus on endogenous eccrine secretions. To assess the suitability of the immunogenic reagents for potential operational use, a variety of parameters (i.e., processing time, fixing and working solution conditions) were optimised on a wide range of non-porous and semi-porous substrates. The relative performance of immunogenic reagents was compared to that of routine techniques applied to latent marks and marks in blood, semen and saliva. The incorporation of these novel reagents into routine technique sequences was also investigated. The experimental results indicated that the multi-target immunogenic reagents were not a suitable alternative to routine detection methods or sequences, but may have promise as a “last resort” method for difficult substrates or cases.	en_US
dc.relation	http://purl.org/au-research/grants/arc/LP130101019
dc.relation.ispartof	Forensic Science International	en_US
dc.relation.isbasedon	10.1016/j.forsciint.2016.04.014	en_US
dc.subject.classification	Legal & Forensic Medicine	en_US
dc.subject.mesh	Saliva	en_US
dc.subject.mesh	Semen	en_US
dc.subject.mesh	Blood	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Antibodies	en_US
dc.subject.mesh	Fluorescent Dyes	en_US
dc.subject.mesh	Dermatoglyphics	en_US
dc.subject.mesh	Surface Properties	en_US
dc.subject.mesh	Porosity	en_US
dc.subject.mesh	Aptamers, Nucleotide	en_US
dc.title	Evaluation of multi-target immunogenic reagents for the detection of latent and body fluid-contaminated fingermarks	en_US
dc.type	Journal Article
utslib.citation.volume	264	en_US
utslib.for	039902 Forensic Chemistry	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	264	en_US

Abstract:

© 2016 Elsevier Ireland Ltd Fingermark enhancement reagents capable of molecular recognition offer a highly selective and sensitive method of detection. Antibodies and aptamers provide a high degree of adaptability for visualisation, allowing for the selection of the most appropriate visualisation wavelength for a particular substrate without the need for specialist equipment or image processing. However, the major hurdle to overcome is the balance between sensitivity and selectivity. Single-target molecular recognition is highly specific, purported to have better detection limits than chemical reactions or stains, and can provide information about the donor or activity, but often results in incomplete ridge pattern development. Consequently, the development and evaluation of multi-target biomolecular reagents for fingermark enhancement was investigated, with the focus on endogenous eccrine secretions. To assess the suitability of the immunogenic reagents for potential operational use, a variety of parameters (i.e., processing time, fixing and working solution conditions) were optimised on a wide range of non-porous and semi-porous substrates. The relative performance of immunogenic reagents was compared to that of routine techniques applied to latent marks and marks in blood, semen and saliva. The incorporation of these novel reagents into routine technique sequences was also investigated. The experimental results indicated that the multi-target immunogenic reagents were not a suitable alternative to routine detection methods or sequences, but may have promise as a “last resort” method for difficult substrates or cases.

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http://hdl.handle.net/10453/117980