Comparative performance of four immunological test kits for the detection of Paralytic Shellfish Toxins in Tasmanian shellfish

Dorantes-Aranda, JJ; Campbell, K; Bradbury, A; Elliott, CT; Harwood, DT; Murray, SA; Ugalde, SC; Wilson, K; Burgoyne, M; Hallegraeff, GM

Comparative performance of four immunological test kits for the detection of Paralytic Shellfish Toxins in Tasmanian shellfish

Dorantes-Aranda, JJ Campbell, K Bradbury, A Elliott, CT Harwood, DT Murray, SA

Ugalde, SC Wilson, K Burgoyne, M Hallegraeff, GM

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Publication Type:: Journal Article
Citation:: Toxicon, 2017, 125 pp. 110 - 119
Issue Date:: 2017-01-01

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Field	Value	Language
dc.contributor.author	Dorantes-Aranda, JJ	en_US
dc.contributor.author	Campbell, K	en_US
dc.contributor.author	Bradbury, A	en_US
dc.contributor.author	Elliott, CT	en_US
dc.contributor.author	Harwood, DT	en_US
dc.contributor.author	Murray, SA https://orcid.org/0000-0001-7096-1307	en_US
dc.contributor.author	Ugalde, SC	en_US
dc.contributor.author	Wilson, K	en_US
dc.contributor.author	Burgoyne, M	en_US
dc.contributor.author	Hallegraeff, GM	en_US
dc.date.available	2016-11-29	en_US
dc.date.issued	2017-01-01	en_US
dc.identifier.citation	Toxicon, 2017, 125 pp. 110 - 119	en_US
dc.identifier.issn	0041-0101	en_US
dc.identifier.uri	http://hdl.handle.net/10453/118048
dc.description.abstract	© 2016 Elsevier Ltd Blooms of the toxic dinoflagellate Alexandrium tamarense (Group 1) seriously impacted the Tasmanian shellfish industry during 2012 and 2015, necessitating product recalls and intensive paralytic shellfish toxin (PST) product testing. The performance of four commercial PST test kits, Abraxis™, Europroxima™, Scotia™ and Neogen™, was compared with the official AOAC LC-FLD method for contaminated mussels and oysters. Abraxis and Europroxima kits underestimated PST in 35–100% of samples when using standard protocols but quantification improved when concentrated extracts were further diluted (underestimation ≤18%). The Scotia kit (cut off 0.2–0.7 mg STX-diHCl eq/kg) delivered 0% false negatives, but 27% false positives. Neogen produced 5% false negatives and 13% false positives when the cut off was altered to 0.5–0.6 mg STX-diHCl eq/kg, the introduction of a conversion step eliminated false negatives. Based on their sensitivity, ease of use and performance, the Neogen kit proved the most suitable kit for use with Tasmanian mussels and oysters. Once formally validated for regulatory purposes, the Neogen kit could provide shellfish growers with a rapid tool for harvesting decisions at the farm gate. Effective rapid screening preventing compliant samples undergoing testing using the more expensive and time consuming LC-FLD method will result in significant savings in analytical costs.	en_US
dc.relation.ispartof	Toxicon	en_US
dc.relation.isbasedon	10.1016/j.toxicon.2016.11.262	en_US
dc.subject.classification	Toxicology	en_US
dc.subject.mesh	Dinoflagellida	en_US
dc.subject.mesh	Marine Toxins	en_US
dc.subject.mesh	Enzyme-Linked Immunosorbent Assay	en_US
dc.subject.mesh	Environmental Monitoring	en_US
dc.subject.mesh	Shellfish	en_US
dc.subject.mesh	Tasmania	en_US
dc.subject.mesh	Shellfish Poisoning	en_US
dc.subject.mesh	Hazard Analysis and Critical Control Points	en_US
dc.title	Comparative performance of four immunological test kits for the detection of Paralytic Shellfish Toxins in Tasmanian shellfish	en_US
dc.type	Journal Article
utslib.citation.volume	125	en_US
utslib.for	1115 Pharmacology and Pharmaceutical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	125	en_US

Abstract:

© 2016 Elsevier Ltd Blooms of the toxic dinoflagellate Alexandrium tamarense (Group 1) seriously impacted the Tasmanian shellfish industry during 2012 and 2015, necessitating product recalls and intensive paralytic shellfish toxin (PST) product testing. The performance of four commercial PST test kits, Abraxis™, Europroxima™, Scotia™ and Neogen™, was compared with the official AOAC LC-FLD method for contaminated mussels and oysters. Abraxis and Europroxima kits underestimated PST in 35–100% of samples when using standard protocols but quantification improved when concentrated extracts were further diluted (underestimation ≤18%). The Scotia kit (cut off 0.2–0.7 mg STX-diHCl eq/kg) delivered 0% false negatives, but 27% false positives. Neogen produced 5% false negatives and 13% false positives when the cut off was altered to 0.5–0.6 mg STX-diHCl eq/kg, the introduction of a conversion step eliminated false negatives. Based on their sensitivity, ease of use and performance, the Neogen kit proved the most suitable kit for use with Tasmanian mussels and oysters. Once formally validated for regulatory purposes, the Neogen kit could provide shellfish growers with a rapid tool for harvesting decisions at the farm gate. Effective rapid screening preventing compliant samples undergoing testing using the more expensive and time consuming LC-FLD method will result in significant savings in analytical costs.

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http://hdl.handle.net/10453/118048