Three-dimensional analysis of the vestibular end organs in relation to the stapes footplate and piston placement

Mukherjee, P; Uzun-Coruhlu, H; Curthoys, IS; Jones, AS; Bradshaw, AP; Pohl, DV

Three-dimensional analysis of the vestibular end organs in relation to the stapes footplate and piston placement

Mukherjee, P Uzun-Coruhlu, H Curthoys, IS Jones, AS

Bradshaw, AP Pohl, DV

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Publication Type:: Journal Article
Citation:: Otology and Neurotology, 2011, 32 (3), pp. 367 - 372
Issue Date:: 2011-04-01

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Field	Value	Language
dc.contributor.author	Mukherjee, P	en_US
dc.contributor.author	Uzun-Coruhlu, H	en_US
dc.contributor.author	Curthoys, IS	en_US
dc.contributor.author	Jones, AS https://orcid.org/0000-0002-3522-6371	en_US
dc.contributor.author	Bradshaw, AP	en_US
dc.contributor.author	Pohl, DV	en_US
dc.date.issued	2011-04-01	en_US
dc.identifier.citation	Otology and Neurotology, 2011, 32 (3), pp. 367 - 372	en_US
dc.identifier.issn	1531-7129	en_US
dc.identifier.uri	http://hdl.handle.net/10453/118107
dc.description.abstract	Objective: Measurements of the proximity of the membranous labyrinth to the stapes footplate show considerable variation. Largely, such measurements have been from histologic sections of fixed temporal bones, which may be affected by shrinkage artifact and perspective distortion in the 2-dimensional plane. To overcome these problems, the present study undertook an analysis of the 3-dimensional (3D) architecture of the relationship of the stapes to the membranous labyrinth using high-resolution X-ray micro-computed tomography. METHODS: Eleven temporal bones were fixed with Karnovsky's fixative (known to minimize shrinkage), soaked in 2% osmium tetroxide, and scanned in a micro-computed tomography scanner. The otic capsule was intact to exclude sectioning artifact, and no alcohol was used to avoid tissue shrinkage. Measurements were taken in a vertical plane to provide distances from the utricle and saccule to the footplate, and 3D reconstruction of the spatial relationship of these structures was carried out. The relationship of these structures to a stapes piston also was studied. RESULTS: The safest area of piston placement was the central and inferior part of the footplate. This was safe up to 0.5 mm depth at all areas except posterosuperiorly where the utricular macula is, on average, only 0.61 mm away from the footplate. The angle of insertion of the piston also influences the end result. Conclusion: Two-dimensional information about vestibular end organ location should serve as a guideline only because the operative field is 3D, and the relationship of the piston to the vestibular labyrinth changes with the angle of placement. Copyright © 2011 Otology & Neurotology, Inc. Unauthorized reproduction of this article is prohibited.	en_US
dc.relation.ispartof	Otology and Neurotology	en_US
dc.relation.isbasedon	10.1097/MAO.0b013e3182096ddd	en_US
dc.subject.classification	Otorhinolaryngology	en_US
dc.subject.mesh	Temporal Bone	en_US
dc.subject.mesh	Stapes	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Imaging, Three-Dimensional	en_US
dc.subject.mesh	Vestibule, Labyrinth	en_US
dc.subject.mesh	X-Ray Microtomography	en_US
dc.title	Three-dimensional analysis of the vestibular end organs in relation to the stapes footplate and piston placement	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	32	en_US
utslib.for	0608 Zoology	en_US
utslib.for	1103 Clinical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	32	en_US

Abstract:

Objective: Measurements of the proximity of the membranous labyrinth to the stapes footplate show considerable variation. Largely, such measurements have been from histologic sections of fixed temporal bones, which may be affected by shrinkage artifact and perspective distortion in the 2-dimensional plane. To overcome these problems, the present study undertook an analysis of the 3-dimensional (3D) architecture of the relationship of the stapes to the membranous labyrinth using high-resolution X-ray micro-computed tomography. METHODS: Eleven temporal bones were fixed with Karnovsky's fixative (known to minimize shrinkage), soaked in 2% osmium tetroxide, and scanned in a micro-computed tomography scanner. The otic capsule was intact to exclude sectioning artifact, and no alcohol was used to avoid tissue shrinkage. Measurements were taken in a vertical plane to provide distances from the utricle and saccule to the footplate, and 3D reconstruction of the spatial relationship of these structures was carried out. The relationship of these structures to a stapes piston also was studied. RESULTS: The safest area of piston placement was the central and inferior part of the footplate. This was safe up to 0.5 mm depth at all areas except posterosuperiorly where the utricular macula is, on average, only 0.61 mm away from the footplate. The angle of insertion of the piston also influences the end result. Conclusion: Two-dimensional information about vestibular end organ location should serve as a guideline only because the operative field is 3D, and the relationship of the piston to the vestibular labyrinth changes with the angle of placement. Copyright © 2011 Otology & Neurotology, Inc. Unauthorized reproduction of this article is prohibited.

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http://hdl.handle.net/10453/118107