Effects of chrysolaminarin synthase knockdown in the diatom Thalassiosira pseudonana: Implications of reduced carbohydrate storage relative to green algae

Hildebrand, M; Manandhar-Shrestha, K; Abbriano, R

Effects of chrysolaminarin synthase knockdown in the diatom Thalassiosira pseudonana: Implications of reduced carbohydrate storage relative to green algae

Hildebrand, M Manandhar-Shrestha, K Abbriano, R

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Publication Type:: Journal Article
Citation:: Algal Research, 2017, 23 pp. 66 - 77
Issue Date:: 2017-04-01

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Field	Value	Language
dc.contributor.author	Hildebrand, M	en_US
dc.contributor.author	Manandhar-Shrestha, K	en_US
dc.contributor.author	Abbriano, R https://orcid.org/0000-0002-5754-5461	en_US
dc.date.issued	2017-04-01	en_US
dc.identifier.citation	Algal Research, 2017, 23 pp. 66 - 77	en_US
dc.identifier.issn	2211-9264	en_US
dc.identifier.uri	http://hdl.handle.net/10453/118116
dc.description.abstract	© 2017 In all organisms, the flux of carbon through the fundamental pathways of glycolysis, gluconeogenesis and the pyruvate hub is a core process related to growth and productivity. In unicellular microalgae, the complexity and intracellular location of specific steps of these pathways can vary substantially. In addition, the location and chemical nature of storage carbohydrate can be substantially different. The role of starch storage in green algae has been investigated, but thus far, only a minimal understanding of the role of carbohydrate storage in diatoms as the β-1,3-glucan chrysolaminarin has been achieved. In this report, we aimed to determine the effect of specifically reducing the ability of Thalassiosira pseudonana cells to accumulate chrysolaminarin by knocking down transcript levels of the chrysolaminarin synthase gene. We monitored changes in chrysolaminarin and triacylglycerol (TAG) levels during growth and silicon starvation. Transcript-level changes in genes encoding steps in chrysolaminarin metabolism, and cytoplasmic and chloroplast glycolysis/gluconeogenesis, were monitored during silicon limitation, highlighting the carbon flux processes involved. We demonstrate that knockdown lines accumulate less chrysolaminarin and have a transiently increased TAG level, with minimal detriment to growth. The results provide insight into the role of chrysolaminarin storage in diatoms, and further discussion highlights differences between diatoms and green algae in carbohydrate storage processes and the effect of reducing carbohydrate stores on growth and productivity.	en_US
dc.relation.ispartof	Algal Research	en_US
dc.relation.isbasedon	10.1016/j.algal.2017.01.010	en_US
dc.title	Effects of chrysolaminarin synthase knockdown in the diatom Thalassiosira pseudonana: Implications of reduced carbohydrate storage relative to green algae	en_US
dc.type	Journal Article
utslib.citation.volume	23	en_US
utslib.for	0305 Organic Chemistry	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0904 Chemical Engineering	en_US
utslib.for	1003 Industrial Biotechnology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	23	en_US

Abstract:

© 2017 In all organisms, the flux of carbon through the fundamental pathways of glycolysis, gluconeogenesis and the pyruvate hub is a core process related to growth and productivity. In unicellular microalgae, the complexity and intracellular location of specific steps of these pathways can vary substantially. In addition, the location and chemical nature of storage carbohydrate can be substantially different. The role of starch storage in green algae has been investigated, but thus far, only a minimal understanding of the role of carbohydrate storage in diatoms as the β-1,3-glucan chrysolaminarin has been achieved. In this report, we aimed to determine the effect of specifically reducing the ability of Thalassiosira pseudonana cells to accumulate chrysolaminarin by knocking down transcript levels of the chrysolaminarin synthase gene. We monitored changes in chrysolaminarin and triacylglycerol (TAG) levels during growth and silicon starvation. Transcript-level changes in genes encoding steps in chrysolaminarin metabolism, and cytoplasmic and chloroplast glycolysis/gluconeogenesis, were monitored during silicon limitation, highlighting the carbon flux processes involved. We demonstrate that knockdown lines accumulate less chrysolaminarin and have a transiently increased TAG level, with minimal detriment to growth. The results provide insight into the role of chrysolaminarin storage in diatoms, and further discussion highlights differences between diatoms and green algae in carbohydrate storage processes and the effect of reducing carbohydrate stores on growth and productivity.

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http://hdl.handle.net/10453/118116