Differential responses of orexigenic neuropeptides to fasting in offspring of obese mothers

Chen, H; Morris, MJ

Differential responses of orexigenic neuropeptides to fasting in offspring of obese mothers

Chen, H

Morris, MJ

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Publication Type:: Journal Article
Citation:: Obesity, 2009, 17 (7), pp. 1356 - 1362
Issue Date:: 2009-07-01

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Field	Value	Language
dc.contributor.author	Chen, H https://orcid.org/0000-0001-6883-3752	en_US
dc.contributor.author	Morris, MJ	en_US
dc.date.issued	2009-07-01	en_US
dc.identifier.citation	Obesity, 2009, 17 (7), pp. 1356 - 1362	en_US
dc.identifier.issn	1930-7381	en_US
dc.identifier.uri	http://hdl.handle.net/10453/11813
dc.description.abstract	Maternal obesity due to long-term high-fat diet (HFD) consumption leads to faster growth in offspring during suckling, and increased adiposity at 20 days of age. Decreased expression of the orexigenic neuropeptide Y (NPY) and increased anorexigenic proopiomelanocortin (POMC) mRNA expression were observed in the fed state. However, hunger is the major drive to eat and hypothalamic appetite regulators change in response to meals. Therefore, it is important to compare both satiated and fasting states. Female Sprague-Dawley rats (8 weeks old) were fed a cafeteria-style HFD (15.33 kJ/g) or chow for 5 weeks before mating, with the same diet continuing throughout gestation and lactation. At postnatal day 20, male pups were killed either after overnight fasting or in the fed state. Pups from obese dams were hyperphagic during both pre- and postweaning periods. Pups from obese dams had higher hypothalamic mRNA expression of POMC and NPY Y1 receptor, but lower hypothalamic melanocortin-4 receptor (MC4R) and its downstream target single-minded gene 1 (Sim1), in the fed state. Overnight fasting reduced circulating glucose, insulin, and leptin and increased hypothalamic NPY Y1 receptor mRNA in pups from both lean and obese dams. Hypothalamic NPY and agouti-related protein (AgRP) were only increased by fasting in pups from obese dams; reductions in MC4R and Sim1 were only seen in pups from lean dams. At weaning, the suppressed orexigenic signals in offspring from obese dams were normalized after overnight fasting, although anorexigenic signaling appeared impaired in these animals. This may contribute to their hyperphagia and faster growth. © 2009 The Obesity Society.	en_US
dc.relation.ispartof	Obesity	en_US
dc.relation.isbasedon	10.1038/oby.2009.56	en_US
dc.subject.classification	Endocrinology & Metabolism	en_US
dc.subject.mesh	Hypothalamus	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Animals, Newborn	en_US
dc.subject.mesh	Rats	en_US
dc.subject.mesh	Rats, Sprague-Dawley	en_US
dc.subject.mesh	Prenatal Exposure Delayed Effects	en_US
dc.subject.mesh	Obesity	en_US
dc.subject.mesh	Disease Models, Animal	en_US
dc.subject.mesh	Hyperphagia	en_US
dc.subject.mesh	Insulin	en_US
dc.subject.mesh	Leptin	en_US
dc.subject.mesh	Pro-Opiomelanocortin	en_US
dc.subject.mesh	Blood Glucose	en_US
dc.subject.mesh	Neuropeptide Y	en_US
dc.subject.mesh	Receptor, Melanocortin, Type 4	en_US
dc.subject.mesh	Repressor Proteins	en_US
dc.subject.mesh	RNA, Messenger	en_US
dc.subject.mesh	Fasting	en_US
dc.subject.mesh	Pregnancy	en_US
dc.subject.mesh	Appetite Regulation	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Basic Helix-Loop-Helix Transcription Factors	en_US
dc.subject.mesh	Agouti-Related Protein	en_US
dc.subject.mesh	Maternal Nutritional Physiological Phenomena	en_US
dc.title	Differential responses of orexigenic neuropeptides to fasting in offspring of obese mothers	en_US
dc.type	Journal Article
utslib.citation.volume	7	en_US
utslib.citation.volume	17	en_US
utslib.for	1111 Nutrition and Dietetics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.issue	7	en_US
pubs.publication-status	Published	en_US
pubs.volume	17	en_US

Abstract:

Maternal obesity due to long-term high-fat diet (HFD) consumption leads to faster growth in offspring during suckling, and increased adiposity at 20 days of age. Decreased expression of the orexigenic neuropeptide Y (NPY) and increased anorexigenic proopiomelanocortin (POMC) mRNA expression were observed in the fed state. However, hunger is the major drive to eat and hypothalamic appetite regulators change in response to meals. Therefore, it is important to compare both satiated and fasting states. Female Sprague-Dawley rats (8 weeks old) were fed a cafeteria-style HFD (15.33 kJ/g) or chow for 5 weeks before mating, with the same diet continuing throughout gestation and lactation. At postnatal day 20, male pups were killed either after overnight fasting or in the fed state. Pups from obese dams were hyperphagic during both pre- and postweaning periods. Pups from obese dams had higher hypothalamic mRNA expression of POMC and NPY Y1 receptor, but lower hypothalamic melanocortin-4 receptor (MC4R) and its downstream target single-minded gene 1 (Sim1), in the fed state. Overnight fasting reduced circulating glucose, insulin, and leptin and increased hypothalamic NPY Y1 receptor mRNA in pups from both lean and obese dams. Hypothalamic NPY and agouti-related protein (AgRP) were only increased by fasting in pups from obese dams; reductions in MC4R and Sim1 were only seen in pups from lean dams. At weaning, the suppressed orexigenic signals in offspring from obese dams were normalized after overnight fasting, although anorexigenic signaling appeared impaired in these animals. This may contribute to their hyperphagia and faster growth. © 2009 The Obesity Society.

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http://hdl.handle.net/10453/11813