Applications of imaging flow cytometry for microalgae

Hildebrand, M; Davis, A; Abbriano, R; Pugsley, HR; Traller, JC; Smith, SR; Shrestha, RP; Cook, O; Sánchez-Alvarez, EL; Manandhar-Shrestha, K; Alderete, B

Applications of imaging flow cytometry for microalgae

Hildebrand, M Davis, A Abbriano, R

Pugsley, HR Traller, JC Smith, SR Shrestha, RP Cook, O Sánchez-Alvarez, EL Manandhar-Shrestha, K Alderete, B

Permalink

Publication Type:: Chapter
Citation:: 2016, 1389 pp. 47 - 67
Issue Date:: 2016-01-01

Closed Access

	Filename	Description	Size
	Front matter - Imaging Flow Cytometry.pdf	Published version	311.95 kB	Adobe PDF	View/Open
	2016 Imaging flow cytometry chapter.pdf	Published version	1.07 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Hildebrand, M	en_US
dc.contributor.author	Davis, A	en_US
dc.contributor.author	Abbriano, R https://orcid.org/0000-0002-5754-5461	en_US
dc.contributor.author	Pugsley, HR	en_US
dc.contributor.author	Traller, JC	en_US
dc.contributor.author	Smith, SR	en_US
dc.contributor.author	Shrestha, RP	en_US
dc.contributor.author	Cook, O	en_US
dc.contributor.author	Sánchez-Alvarez, EL	en_US
dc.contributor.author	Manandhar-Shrestha, K	en_US
dc.contributor.author	Alderete, B	en_US
dc.date.issued	2016-01-01	en_US
dc.identifier.citation	2016, 1389 pp. 47 - 67	en_US
dc.identifier.uri	http://hdl.handle.net/10453/118172
dc.description.abstract	© Springer Science+Business Media New York 2016. The ability to image large numbers of cells at high resolution enhances flow cytometric analysis of cells and cell populations. In particular, the ability to image intracellular features adds a unique aspect to analyses, and can enable correlation between molecular phenomena resulting in alterations in cellular phenotype. Unicellular microalgae are amenable to high-throughput analysis to capture the diversity of cell types in natural samples, or diverse cellular responses in clonal populations, especially using imaging cytometry. Using examples from our laboratory, we review applications of imaging cytometry, specifically using an Amnis® ImageStream® X instrument, to characterize photosynthetic microalgae. Some of these examples highlight advantages of imaging flow cytometry for certain research objectives, but we also include examples that would not necessarily require imaging and could be performed on a conventional cytometer to demonstrate other concepts in cytometric evaluation of microalgae. We demonstrate the value of these approaches for (1) analysis of populations, (2) documentation of cellular features, and (3) analysis of gene expression.	en_US
dc.relation.isbasedon	10.1007/978-1-4939-3302-0_4	en_US
dc.subject.classification	Developmental Biology	en_US
dc.subject.mesh	Chlorella	en_US
dc.subject.mesh	Flow Cytometry	en_US
dc.subject.mesh	Image Cytometry	en_US
dc.subject.mesh	Microalgae	en_US
dc.title	Applications of imaging flow cytometry for microalgae	en_US
dc.type	Chapter
utslib.citation.volume	1389	en_US
utslib.for	0399 Other Chemical Sciences	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	1389	en_US

Abstract:

© Springer Science+Business Media New York 2016. The ability to image large numbers of cells at high resolution enhances flow cytometric analysis of cells and cell populations. In particular, the ability to image intracellular features adds a unique aspect to analyses, and can enable correlation between molecular phenomena resulting in alterations in cellular phenotype. Unicellular microalgae are amenable to high-throughput analysis to capture the diversity of cell types in natural samples, or diverse cellular responses in clonal populations, especially using imaging cytometry. Using examples from our laboratory, we review applications of imaging cytometry, specifically using an Amnis® ImageStream® X instrument, to characterize photosynthetic microalgae. Some of these examples highlight advantages of imaging flow cytometry for certain research objectives, but we also include examples that would not necessarily require imaging and could be performed on a conventional cytometer to demonstrate other concepts in cytometric evaluation of microalgae. We demonstrate the value of these approaches for (1) analysis of populations, (2) documentation of cellular features, and (3) analysis of gene expression.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/118172