The effects and mechanism of saponins of panax notoginseng on glucose metabolism in 3T3-L1 cells

Kim, JJY; Xiao, H; Tan, Y; Wang, ZZ; Paul Seale, J; Qu, X

The effects and mechanism of saponins of panax notoginseng on glucose metabolism in 3T3-L1 cells

Kim, JJY Xiao, H Tan, Y

Wang, ZZ Paul Seale, J Qu, X

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Publication Type:: Journal Article
Citation:: American Journal of Chinese Medicine, 2009, 37 (6), pp. 1179 - 1189
Issue Date:: 2009-12-01

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Field	Value	Language
dc.contributor.author	Kim, JJY	en_US
dc.contributor.author	Xiao, H	en_US
dc.contributor.author	Tan, Y https://orcid.org/0000-0001-9221-330X	en_US
dc.contributor.author	Wang, ZZ	en_US
dc.contributor.author	Paul Seale, J	en_US
dc.contributor.author	Qu, X	en_US
dc.date.issued	2009-12-01	en_US
dc.identifier.citation	American Journal of Chinese Medicine, 2009, 37 (6), pp. 1179 - 1189	en_US
dc.identifier.issn	0192-415X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/11818
dc.description.abstract	This study was carried out to determine the effect of saponins of Panax notoginseng (SPN), a naturally occurring cardiovascular agent, on: (1) glucose uptake, (2) GLUT4 translocation and (3) glycogen synthesis in 3T3-L1 adipocytes. Electrospray ionization-Mass spectrometry (ESI-MS) was used to determine the structural characterization of the major active components of SPN. 3T3-L1 adipocytes were cultured and treated with 100 nM insulin alone or with 10, 50 and 100 μg/ml of SPN. [3H]2-deoxyglucose glucose uptake, GLUT4 immunofluorescence imaging and glycogen synthesis assay were carried out to determine the effects of SPN on glucose metabolism. Under insulin stimulation, SPN significantly increased glucose uptake in a dose-dependent manner; 50 μg/ml of SPN increased glucose uptake by 64% (p < 0.001). Immunofluorescence imaging and analysis have revealed that 50 and 100 μg/ml of SPN increased GLUT4 in the plasma membrane by 3-fold and 6-fold respectively (p < 0.001). Furthermore, the incorporation of D-[U-14C] glucose into glycogen was enhanced by 53% in 3T3-L1 cells treated with 100 μg/ml of SPN (p < 0.01 vs. insulin stimulation alone). SPN, a naturally occurring agent used to treat ischemic cardio-cerebral vascular disease in China, enhanced insulin-stimulated glucose uptake and glycogen synthesis in adipocytes. The results of this study indicate that SPN may have a therapeutic potential for hyperglycaemia in type 2 diabetes. © 2009 World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine.	en_US
dc.relation.ispartof	American Journal of Chinese Medicine	en_US
dc.relation.isbasedon	10.1142/S0192415X09007582	en_US
dc.subject.classification	Complementary & Alternative Medicine	en_US
dc.subject.mesh	3T3-L1 Cells	en_US
dc.subject.mesh	Cell Membrane	en_US
dc.subject.mesh	Adipocytes	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Glycogen	en_US
dc.subject.mesh	Insulin	en_US
dc.subject.mesh	Saponins	en_US
dc.subject.mesh	Glucose	en_US
dc.subject.mesh	Plant Extracts	en_US
dc.subject.mesh	Hypoglycemic Agents	en_US
dc.subject.mesh	Biological Transport	en_US
dc.subject.mesh	Dose-Response Relationship, Drug	en_US
dc.subject.mesh	Drug Synergism	en_US
dc.subject.mesh	Glucose Transporter Type 4	en_US
dc.subject.mesh	Panax notoginseng	en_US
dc.title	The effects and mechanism of saponins of panax notoginseng on glucose metabolism in 3T3-L1 cells	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	37	en_US
utslib.for	110404 Traditional Chinese Medicine and Treatments	en_US
utslib.for	11 Medical and Health Sciences	en_US
dc.location.activity	ISI:000272093600014	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Mechanical and Mechatronic Engineering
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	37	en_US

Abstract:

This study was carried out to determine the effect of saponins of Panax notoginseng (SPN), a naturally occurring cardiovascular agent, on: (1) glucose uptake, (2) GLUT4 translocation and (3) glycogen synthesis in 3T3-L1 adipocytes. Electrospray ionization-Mass spectrometry (ESI-MS) was used to determine the structural characterization of the major active components of SPN. 3T3-L1 adipocytes were cultured and treated with 100 nM insulin alone or with 10, 50 and 100 μg/ml of SPN. [3H]2-deoxyglucose glucose uptake, GLUT4 immunofluorescence imaging and glycogen synthesis assay were carried out to determine the effects of SPN on glucose metabolism. Under insulin stimulation, SPN significantly increased glucose uptake in a dose-dependent manner; 50 μg/ml of SPN increased glucose uptake by 64% (p < 0.001). Immunofluorescence imaging and analysis have revealed that 50 and 100 μg/ml of SPN increased GLUT4 in the plasma membrane by 3-fold and 6-fold respectively (p < 0.001). Furthermore, the incorporation of D-[U-14C] glucose into glycogen was enhanced by 53% in 3T3-L1 cells treated with 100 μg/ml of SPN (p < 0.01 vs. insulin stimulation alone). SPN, a naturally occurring agent used to treat ischemic cardio-cerebral vascular disease in China, enhanced insulin-stimulated glucose uptake and glycogen synthesis in adipocytes. The results of this study indicate that SPN may have a therapeutic potential for hyperglycaemia in type 2 diabetes. © 2009 World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine.

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http://hdl.handle.net/10453/11818