Maternal E-cigarette exposure in mice alters DNA methylation and lung cytokine expression in offspring

Chen, H; Li, G; Chan, YL; Chapman, DG; Sukjamnong, S; Nguyen, T; Annissa, T; McGrath, KC; Sharma, P; Oliver, BG

Maternal E-cigarette exposure in mice alters DNA methylation and lung cytokine expression in offspring

Chen, H

Li, G Chan, YL

Chapman, DG

Sukjamnong, S Nguyen, T

Annissa, T McGrath, KC

Sharma, P

Oliver, BG

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Publication Type:: Journal Article
Citation:: American Journal of Respiratory Cell and Molecular Biology, 2018, 58 (3), pp. 366 - 377
Issue Date:: 2018-03-01

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Field	Value	Language
dc.contributor.author	Chen, H https://orcid.org/0000-0001-6883-3752	en_US
dc.contributor.author	Li, G	en_US
dc.contributor.author	Chan, YL https://orcid.org/0000-0002-9605-4200	en_US
dc.contributor.author	Chapman, DG https://orcid.org/0000-0002-8211-1817	en_US
dc.contributor.author	Sukjamnong, S	en_US
dc.contributor.author	Nguyen, T https://orcid.org/0000-0001-6371-4105	en_US
dc.contributor.author	Annissa, T	en_US
dc.contributor.author	McGrath, KC https://orcid.org/0000-0002-6244-3929	en_US
dc.contributor.author	Sharma, P https://orcid.org/0000-0002-2904-2306	en_US
dc.contributor.author	Oliver, BG https://orcid.org/0000-0002-7122-9262	en_US
dc.date.issued	2018-03-01	en_US
dc.identifier.citation	American Journal of Respiratory Cell and Molecular Biology, 2018, 58 (3), pp. 366 - 377	en_US
dc.identifier.issn	1044-1549	en_US
dc.identifier.uri	http://hdl.handle.net/10453/118272
dc.description.abstract	Copyright © 2018 by the American Thoracic Society E-cigarette usage is increasing, especially among the young, with both the general population and physicians perceiving them as a safe alternative to tobacco smoking. Worryingly, e-cigarettes are commonly used by pregnant women. As nicotine is known to adversely affect children in utero, we hypothesized that nicotine delivered via e-cigarettes would negatively affect lung development. To test this, we developed a mouse model of maternal e-vapor (nicotine and nicotine-free) exposure and investigated the impact on the growth and lung inflammation in both offspring and mothers. Female Balb/c mice were exposed to e-fluid vapor containing nicotine (18 mg/ml nicotine E-cigarette [E-cig18], equivalent to two cigarettes per treatment, twice daily,) or nicotine free (E-cig0 mg/ml) from 6 weeks before mating until pups weaned. Male offspring were studied at Postnatal Day (P) 1, P20, and at 13 weeks. The mothers were studied when the pups weaned. In the mothers' lungs, e-cigarette exposure with and without nicotine increased the proinflammatory cytokines IL-1b, IL-6, and TNF-a. In adult offspring, TNF-a protein levels were increased in both E-cig18 and E-cig0 groups, whereas IL-1b was suppressed. This was accompanied by global changes in DNA methylation. In this study, we found that e-cigarette exposure during pregnancy adversely affected maternal and offspring lung health. As this occurred with both nicotine-free and nicotine-containing e-vapor, the effects are likely due to by-products of vaporization rather than nicotine.	en_US
dc.relation	http://purl.org/au-research/grants/nhmrc/APP1026880
dc.relation	http://purl.org/au-research/grants/nhmrc/APP1110368
dc.relation.ispartof	American Journal of Respiratory Cell and Molecular Biology	en_US
dc.relation.isbasedon	10.1165/rcmb.2017-0206RC	en_US
dc.subject.classification	Respiratory System	en_US
dc.subject.mesh	Lung	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice, Inbred BALB C	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Prenatal Exposure Delayed Effects	en_US
dc.subject.mesh	Nicotine	en_US
dc.subject.mesh	Tumor Necrosis Factor-alpha	en_US
dc.subject.mesh	Interleukin-6	en_US
dc.subject.mesh	Smoking	en_US
dc.subject.mesh	DNA Methylation	en_US
dc.subject.mesh	Pregnancy	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Interleukin-1beta	en_US
dc.subject.mesh	Electronic Nicotine Delivery Systems	en_US
dc.title	Maternal E-cigarette exposure in mice alters DNA methylation and lung cytokine expression in offspring	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	58	en_US
utslib.for	1102 Cardiorespiratory Medicine and Haematology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	58	en_US

Abstract:

Copyright © 2018 by the American Thoracic Society E-cigarette usage is increasing, especially among the young, with both the general population and physicians perceiving them as a safe alternative to tobacco smoking. Worryingly, e-cigarettes are commonly used by pregnant women. As nicotine is known to adversely affect children in utero, we hypothesized that nicotine delivered via e-cigarettes would negatively affect lung development. To test this, we developed a mouse model of maternal e-vapor (nicotine and nicotine-free) exposure and investigated the impact on the growth and lung inflammation in both offspring and mothers. Female Balb/c mice were exposed to e-fluid vapor containing nicotine (18 mg/ml nicotine E-cigarette [E-cig18], equivalent to two cigarettes per treatment, twice daily,) or nicotine free (E-cig0 mg/ml) from 6 weeks before mating until pups weaned. Male offspring were studied at Postnatal Day (P) 1, P20, and at 13 weeks. The mothers were studied when the pups weaned. In the mothers' lungs, e-cigarette exposure with and without nicotine increased the proinflammatory cytokines IL-1b, IL-6, and TNF-a. In adult offspring, TNF-a protein levels were increased in both E-cig18 and E-cig0 groups, whereas IL-1b was suppressed. This was accompanied by global changes in DNA methylation. In this study, we found that e-cigarette exposure during pregnancy adversely affected maternal and offspring lung health. As this occurred with both nicotine-free and nicotine-containing e-vapor, the effects are likely due to by-products of vaporization rather than nicotine.

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http://hdl.handle.net/10453/118272