A novel hydrogen peroxide biosensor based on hemoglobin-collagen-CNTs composite nanofibers

Li, J; Mei, H; Zheng, W; Pan, P; Sun, XJ; Li, F; Guo, F; Zhou, HM; Ma, JY; Xu, XX; Zheng, YF

A novel hydrogen peroxide biosensor based on hemoglobin-collagen-CNTs composite nanofibers

Li, J Mei, H Zheng, W Pan, P Sun, XJ Li, F Guo, F Zhou, HM Ma, JY Xu, XX

Zheng, YF

Permalink

Publication Type:: Journal Article
Citation:: Colloids and Surfaces B: Biointerfaces, 2014, 118 pp. 77 - 82
Issue Date:: 2014-06-01

Closed Access

	Filename	Description	Size
	1-s2.0-S0927776514001672-main.pdf	Published Version	1.51 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Li, J	en_US
dc.contributor.author	Mei, H	en_US
dc.contributor.author	Zheng, W	en_US
dc.contributor.author	Pan, P	en_US
dc.contributor.author	Sun, XJ	en_US
dc.contributor.author	Li, F	en_US
dc.contributor.author	Guo, F	en_US
dc.contributor.author	Zhou, HM	en_US
dc.contributor.author	Ma, JY	en_US
dc.contributor.author	Xu, XX https://orcid.org/0000-0002-2598-3766	en_US
dc.contributor.author	Zheng, YF	en_US
dc.date.available	2014-03-20	en_US
dc.date.issued	2014-06-01	en_US
dc.identifier.citation	Colloids and Surfaces B: Biointerfaces, 2014, 118 pp. 77 - 82	en_US
dc.identifier.issn	0927-7765	en_US
dc.identifier.uri	http://hdl.handle.net/10453/118877
dc.description.abstract	In this paper, carbon nanotubes (CNTs) were successfully incorporated in the composite composed of hemoglobin (Hb) and collagen using co-electrospinning technology. The formed Hb-collagen-CNTs composite nanofibers possessed distinct advantage of three-dimensional porous structure, biocompatibility and excellent stability. The Hb immobilized in the electrospun nanofibers retained its natural structure and the heterogeneous electron transfer rate constant (k<inf>s</inf>) of the direct electron transfer between Hb and electrodes was 5.3s<sup>-1</sup>. In addition, the electrospun Hb-collagen-CNTs nanofibers modified electrodes showed good electrocatalytic properties toward H<inf>2</inf>O<inf>2</inf> with a detection limit of 0.91μM (signal-to-noise ratio of 3) and the apparent Michaelis-Menten constant (Kmapp) of 32.6μM. © 2014 Elsevier B.V.	en_US
dc.relation.ispartof	Colloids and Surfaces B: Biointerfaces	en_US
dc.relation.isbasedon	10.1016/j.colsurfb.2014.03.035	en_US
dc.subject.classification	Chemical Physics	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Cattle	en_US
dc.subject.mesh	Hydrogen Peroxide	en_US
dc.subject.mesh	Nanotubes, Carbon	en_US
dc.subject.mesh	Collagen	en_US
dc.subject.mesh	Hemoglobins	en_US
dc.subject.mesh	Biosensing Techniques	en_US
dc.subject.mesh	Electrodes	en_US
dc.subject.mesh	Electrons	en_US
dc.subject.mesh	Electrochemical Techniques	en_US
dc.subject.mesh	Immobilized Proteins	en_US
dc.subject.mesh	Nanofibers	en_US
dc.title	A novel hydrogen peroxide biosensor based on hemoglobin-collagen-CNTs composite nanofibers	en_US
dc.type	Journal Article
utslib.citation.volume	118	en_US
utslib.for	0306 Physical Chemistry (incl. Structural)	en_US
utslib.for	0904 Chemical Engineering	en_US
utslib.for	0903 Biomedical Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	118	en_US

Abstract:

In this paper, carbon nanotubes (CNTs) were successfully incorporated in the composite composed of hemoglobin (Hb) and collagen using co-electrospinning technology. The formed Hb-collagen-CNTs composite nanofibers possessed distinct advantage of three-dimensional porous structure, biocompatibility and excellent stability. The Hb immobilized in the electrospun nanofibers retained its natural structure and the heterogeneous electron transfer rate constant (ks) of the direct electron transfer between Hb and electrodes was 5.3s^-1. In addition, the electrospun Hb-collagen-CNTs nanofibers modified electrodes showed good electrocatalytic properties toward H2O2 with a detection limit of 0.91μM (signal-to-noise ratio of 3) and the apparent Michaelis-Menten constant (Kmapp) of 32.6μM. © 2014 Elsevier B.V.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/118877